Low oxygen level increases proliferation and metabolic changes in bovine granulosa cells

Shiratsuki, Shogo; Hara, Tomotaka; Munakata, Yasuhisa; Shirasuna, Koumei; Kuwayama, Takehito; Iwata, Hisataka

doi:10.1016/j.mce.2016.08.010

Cited by 51 publications

(36 citation statements)

References 55 publications

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“…Cellular hypoxia significantly decreased the expression of mitochondria genes . Mitochondria are multifunctional organelles that are important for energy production, apoptosis, and calcium homeostasis .…”

Section: Introductionmentioning

confidence: 99%

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Resveratrol protects mitochondrial quantity by activating SIRT1/PGC‐1α expression during ovarian hypoxia

Nishigaki

Kido

Kida

et al. 2020

Reprod Medicine & Biology

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Purpose Resveratrol is a well‐known potent activator of sirtuin‐1 (SIRT1). We investigated the direct effects of hypoxia and resveratrol on SIRT1/ peroxisome proliferator‐activated receptor‐gamma coactivator 1α (PGC‐1α) pathways, vascular endothelial growth factor (VEGF), hypoxia‐inducible factor (HIF)‐1α, and mitochondrial quantity in a steroidogenic human ovarian granulosa‐like tumor cell line (KGN) cells. Methods KGN cells were cultured with cobalt chloride (CoCl2; a hypoxia‐mimicking agent) and/or resveratrol. The mRNA and protein levels, protein secretion, and intracellular localization were assessed by real‐time PCR, Western blot analysis, ELISA, and immunofluorescence staining, respectively. Mitochondrial quantity was measured based on the mitochondrial DNA (mtDNA) copy number. Results CoCl2 simultaneously attenuated the levels of SIRT1 and mtDNA expression, and induced the levels of VEGF protein production. In contrast, resveratrol significantly increased the levels of SIRT1 and mtDNA copy number, but reduced VEGF production in normoxia. Resveratrol could recover CoCl2‐suppressed SIRT1 and mtDNA expression and antagonize CoCl2‐induced VEGF production. CoCl2 treatment resulted in a downregulation of PGC‐1α expression, and this effect was recovered by resveratrol. Resveratrol significantly suppressed the production of the CoCl2‐induced HIF‐1α and VEGF proteins. Conclusion These results suggest that resveratrol improves mitochondrial quantity by activating the SIRT1/PGC‐1α pathway and inhibits VEGF induction through HIF‐1α under hypoxic conditions.

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Section: Introductionmentioning

confidence: 99%

“…6 Cellular hypoxia significantly decreased the expression of mitochondria genes. 7,8 Mitochondria are multifunctional organelles that are important for energy production, apoptosis, and calcium homeostasis. 9,10 Mitochondrial number is closely related to oocyte maturation, fertilization, and subsequent development.…”

mentioning

confidence: 99%

Resveratrol protects mitochondrial quantity by activating SIRT1/PGC‐1α expression during ovarian hypoxia

Nishigaki

Kido

Kida

et al. 2020

Reprod Medicine & Biology

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“…Hypoxia‐induced increase in VEGFA and HIF1 occurs upstream of glycolysis activation in GCs, and we previously found a metabolic shift in the GCs to glycolysis and activation of HIF1a and VEGFA in the GCs along with follicle development . We therefore examined whether the addition of GCs induced hypoxic conditions in the OGCs, using pimonidazole staining, which reflects the hypoxic conditions of cell.…”

Section: Resultsmentioning

confidence: 99%

Addition of granulosa cells collected from differential follicle stages supports development of oocytes derived from porcine early antral follicles

Ishiguro

Munakata

Shirasuna

et al. 2018

Reprod Medicine & Biology

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PurposeImprovement of in vitro oocyte growth by addition of granulosa cells derived from differential developmental stages of follicles.MethodsGranulosa cells (GCs) collected from either early antral follicles (EAFs) or antral follicles (AFs) were added to oocyte‐granulosa cell complexes (OGCs) derived from EAFs, and the in vitro growth of the oocytes was evaluated.ResultsGranulosa cells were incorporated into OGCs to form new OGCs within 2 days of culture. After 14 days of culture, the number of GCs surrounding oocytes was similar among the three OGCs conditions (unmanipulated “natural OGCs,” “EAF‐GCs add OGCs,” and “AF‐GCs add OGCs”), whereas the survival rate of the GCs and diameter of oocytes grown in vitro were the greatest for “AF‐GCs added OGCs.” After parthenogenetic activation, developmental rate till the blastocyst stage tended to be higher for “AF‐GCs add OGCs” compared with other groups. Addition of AF‐GCs significantly increased a hypoxic marker (pimonidazole staining) and increased the lipid content in oocytes grown in vitro compared with unmanipulated OGCs.ConclusionAddition of GCs derived from more advanced stages of follicles to the OGCs changes the metabolism of oocytes and is beneficial for in vitro growth of oocytes derived from EAFs.

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“…Mitochondria, as a vital organelle in almost all eukaryotic cells, have two major functions: oxidative phosphorylation and ATP production [6, 7]. Accumulating evidences suggested the mitochondria played a key role in the cell proliferation [8], cell apoptosis [9], and aging [10]. More, the mtDNA seemed to be involved in a wide range of diseases such as diabetes [11], obesity [12], AIDS [13], and cancers.…”

Section: Introductionmentioning

confidence: 99%

Can Mitochondria DNA Provide a Novel Biomarker for Evaluating the Risk and Prognosis of Colorectal Cancer?

Han

Pan

2017

Disease Markers

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Colorectal cancer (CRC) was one of the most frequent cancers worldwide. Accurate risk and prognosis evaluation could obtain better quality of life and longer survival time for the patients. Current research hotspot was focus on the gene biomarker to evaluate the risk and prognosis. Mitochondrion contains its own DNA and regulates self-replicating so that it can be as a candidate biomarker for evaluating the risk and prognosis of colorectal cancer. But there were already huge controversies on this issue. The review was to summarize current viewpoints of the controversial issues and described our understanding from the four aspects including mtDNA copy number, mitochondrial displacement loop, mtDNA variation, and mtDNA microsatellite instability, wishing the summary of the mtDNA in colorectal cancer could provide a meaningful reference or a valuable direction in the future studies.

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Low oxygen level increases proliferation and metabolic changes in bovine granulosa cells

Cited by 51 publications

References 55 publications

Resveratrol protects mitochondrial quantity by activating SIRT1/PGC‐1α expression during ovarian hypoxia

Resveratrol protects mitochondrial quantity by activating SIRT1/PGC‐1α expression during ovarian hypoxia

Addition of granulosa cells collected from differential follicle stages supports development of oocytes derived from porcine early antral follicles

Can Mitochondria DNA Provide a Novel Biomarker for Evaluating the Risk and Prognosis of Colorectal Cancer?

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