Low-power laser alters mRNA levels from DNA repair genes in acute lung injury induced by sepsis in Wistar rats

Sergio, Luiz Philippe da Silva; Thomé, Andrezza Maria Côrtes; Trajano, Larissa Alexsandra da Silva Neto; Vicentini, Solange Campos; Teixeira, Adilson Fonseca; Mencalha, André Luiz; Paoli, Flávia de; Fonseca, Adenilson de Souza da

doi:10.1007/s10103-018-2656-9

Cited by 9 publications

(6 citation statements)

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“…Sergio and coworkers [ 84 ] reported that in an experimental model for ALI, by LPS administration, there are reduced relative levels of mRNA from OGG1 gene, suggesting that this could be part of cellular response to sepsis. Lee and coauthors [ 59 ] show the association between mitochondrial DNA damages and ROS cell death is inversely related to the efficiency of a mitochondrial DNA repair, mediated by OGG1.…”

Section: Maintenance Of Genome After Dna Damagementioning

confidence: 99%

“…The reduction of relative levels of ERCC2 and ERCC1 gene messenger RNA in ALI model by LPS administration suggests that these proteins are part of cellular response to sepsis, demonstrating the importance of DNA repair mechanisms in ALI [ 84 ].…”

Section: Maintenance Of Genome After Dna Damagementioning

confidence: 99%

“…OGG1, ERCC1 and ERCC2 were also one of targets of studies by Sergio and coworkers [ 84 ], being suggested that DNA repair is part of cellular response to sepsis observed in an experimental model of ALI by LPS due to exposure to low power infrared laser modulates mRNA levels from BER and NER. These studies indicate that new strategies for treatment of ALI/ARDS are feasible and assessments of DNA repair mechanisms could contribute to improving clinical status in the development of ALI/ARDS ( Fig.…”

Section: Therapeutic Targetsmentioning

confidence: 99%

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DNA repair and genomic stability in lungs affected by acute injury

Sergio

Mencalha

Fonseca

et al. 2019

Biomedicine & Pharmacotherapy

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Section: Maintenance Of Genome After Dna Damagementioning

confidence: 99%

Section: Maintenance Of Genome After Dna Damagementioning

confidence: 99%

Section: Therapeutic Targetsmentioning

confidence: 99%

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DNA repair and genomic stability in lungs affected by acute injury

Sergio

Mencalha

Fonseca

et al. 2019

Biomedicine & Pharmacotherapy

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“…mRNA transcripts from genomic DNA are crucial in many diseases. 23,24 Sergio et al 24 reported that changes in mRNA levels caused by DNA repair and genomic stability might be part of the response of ALI to emphysema and sepsis. However, circRNA and mRNA expression profiles and their combination in the protective effects of P2X 7 R antagonist in sepsis-induced ALI remain undetermined.…”

Section: Introductionmentioning

confidence: 99%

“…reported many circRNAs of dysregulated expression in the pulmonary macrophages of mice with sepsis‐induced ALI. mRNA transcripts from genomic DNA are crucial in many diseases 23,24 . Sergio et al .…”

Section: Introductionmentioning

confidence: 99%

Protective effects of P2X7R antagonist in sepsis‐induced acute lung injury in mice via regulation of circ_0001679 and circ_0001212 and downstream Pln, Cdh2, and Nprl3 expression

Zou

Wang

Zhou

et al. 2020

The Journal of Gene Medicine

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Background: Sepsis induces pulmonary P2X 7 receptor (P2X 7 R) expression and P2X 7 R-knockout reduced lung inflammation in mice. The present study investigated the expression of circular RNA (circRNA) and mRNA in sepsis-induced acute lung injury (ALI) treated with a P2X 7 R antagonist. Methods: Sepsis was induced by tracheal administration of lipopolysaccharide (LPS), and the mice were then divided into two groups: without [sepsis + dimethyl sulfoxide (DMSO)] or with P2X 7 R antagonist treatment (sepsis + P2X 7 A). Sham mice were administrated sterile normal saline. Serum levels of interleukin (IL)-1β and tumor necrosis factor (TNF)-α, pathological changes, cell apoptosis and P2X 7 R expression in lung were assessed, followed by RNA sequencing (RNA-seq) and bioinformatics analyses. A quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to validate circRNAs and mRNAs. Results: Compared to the sham group, LPS-induced sepsis produced obvious pathological changes in lung tissue, as well as increased apoptotic lung cells, serum TNF-α and IL-1β levels, and P2X 7 R expression; P2X 7 R antagonism significantly ameliorated these changes. RNA-seq identified many dysregulated circRNAs and mRNAs during sepsis, whereas this changed with P2X 7 R antagonism. RT-qPCR confirmed that Mus musculus (mmu)_circ_0001679, mmu_circ_0001212, phospholamban (Pln), cadherin-2 (Cdh2) and nitrogen permease regulator 3-like (Nprl3) expression were significantly increased in the sepsis + DMSO group compared to that in the sham group but were decreased in the sepsis + P2X 7 A group compared to that in the sepsis + DMSO group. The circRNA-microRNA-mRNA coexpression network indicated that mmu_circ_0001679 may regulate Nprl3 and that mmu_circ_0001212 may similarly regulate Pln, Cdh2 and Nprl3 as a competing endogenous RNA. Conclusions: P2X 7 R antagonism attenuates sepsis-induced ALI by inhibiting dysregulated expression of circRNA (circ_0001679, circ_0001212) and mRNA (Pln, Cdh2 and Nprl3).

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