Low-pressure, high-temperature thermal bonding of polymeric microfluidic devices and their applications for electrophoretic separation

Sun, Yi; Kwok, Yien Chian; Nguyen, Nam‐Trung

doi:10.1088/0960-1317/16/8/033

Cited by 144 publications

(112 citation statements)

References 23 publications

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“…However, if the speed is set at 100%, channels of 79 ± 1 µm×78 ± 1 µm can be obtained. This inversely proportional relation was somehow expected as the lateral speed controls the number of laser pulses applied per unit area [29]. As it can be seen in Figure 1B, the energy distribution of the laser beam on the surface [26,49] resulted in Gaussian-shaped channels.…”

Section: Microfabrication Of Channels Using Vector Modesupporting

confidence: 57%

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Fast and versatile fabrication of PMMA microchip electrophoretic devices by laser engraving

Gabriel,

Coltro,

Garcia

2014

Electrophoresis

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This paper describes the effects of different modes and engraving parameters on the dimensions of microfluidic structures produced in PMMA using laser engraving. The engraving modes included raster and vector while the explored engraving parameters included power, speed, frequency, resolution, line-width and number of passes. Under the optimum conditions, the technique was applied to produce channels suitable for CE separations. Taking advantage of the possibility to cut-through the substrates, the laser was also used to define solution reservoirs (buffer, sample, and waste) and a PDMS-based decoupler. The final device was used to perform the analysis of a model mixture of phenolic compounds within 200 s with baseline resolution.

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Section: Microfabrication Of Channels Using Vector Modesupporting

confidence: 57%

“…Although traditionally limited by the resolution of the engraver, the process has been explored to fabricate microfluidic components for different applications including injectors [28], channels [29,30], pumps [31], mixers [32], cytometers [33], valves [34], reactors [35], and devices for PCR amplification [36].…”

Section: Introductionmentioning

confidence: 99%

Fast and versatile fabrication of PMMA microchip electrophoretic devices by laser engraving

Gabriel,

Coltro,

Garcia

2014

Electrophoresis

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“…A variety of techniques have been developed for bonding homogeneous or heterogeneous polymer pairings by exposing them to heat 25,26 , microwaves 27 , or solvents 28,29 . Other approaches have used glue 30 , or in situ polymerisation 31 for bonding.…”

Section: Chip Qualitymentioning

confidence: 99%

Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ NMR spectroscopy

Yılmaz

Utz

2016

Lab Chip

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A compact microfludic device for perfusion culture of mammalian cells under in-situ metabolomic observation by NMR spectroscopy is presented. The chip is made from poly(methyl methacrylate) (PMMA), and uses a poly(dimethyl siloxane) (PDMS) membrane to allow gas exchange. It is integrated with a generic micro-NMR detector developed recently in our group [J. Magn. Reson. 262, 73-80 (2016)]. While PMMA is an excellent material in the context of NMR, PDMS is known to produce strong background signals. To mitigate this, the device keeps the PDMS away from the detection area. The oxygen permeation into the device is quantified using a flow chemistry approach. A solution of glucose is mixed on the chip with one of glucose oxidase, before flowing through the gas exchanger. The resulting concentration of gluconate is measured by 1 H NMR spectroscopy as a function of flow rate. An oxygen equilibration rate constant of 2.4 s −1 is found for the device, easily sufficient to maintain normoxic conditions in a cell culture at modest perfusion flow rates.

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“…The fabrication is based on the lamination thermal bonding technique. 21,22 In this method, three polymethylmethacrylate ͑PMMA͒ plates ͑50 mmϫ 50 mm͒ were bonded together to form a closed microfluidic channel with inlet and outlet holes. Thus, the middle PMMA defines the depth of the channel.…”

Section: Experiments On Thermal Mixingmentioning

confidence: 99%

Thermal mixing of two miscible fluids in a T-shaped microchannel

Wong

Nguyen

et al. 2010

Biomicrofluidics

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In this paper, thermal mixing characteristics of two miscible fluids in a T-shaped microchannel are investigated theoretically, experimentally, and numerically. Thermal mixing processes in a T-shaped microchannel are divided into two zones, consisting of a T-junction and a mixing channel. An analytical two-dimensional model was first built to describe the heat transfer processes in the mixing channel.In the experiments, de-ionized water was employed as the working fluid. Laser induced fluorescence method was used to measure the fluid temperature field in the microchannel. Different combinations of flow rate ratios were studied to investigate the thermal mixing characteristics in the microchannel. At the T-junction, thermal diffusion is found to be dominant in this area due to the striation in the temperature contours. In the mixing channel, heat transfer processes are found to be controlled by thermal diffusion and convection. Measured temperature profiles at the T-junction and mixing channel are compared with analytical model and numerical simulation, respectively.

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Low-pressure, high-temperature thermal bonding of polymeric microfluidic devices and their applications for electrophoretic separation

Abstract: Low-pressure, high-temperature thermal bonding of polymeric microfluidic devices and their applications for electrophoretic separation

Cited by 144 publications

References 23 publications

Fast and versatile fabrication of PMMA microchip electrophoretic devices by laser engraving

Fast and versatile fabrication of PMMA microchip electrophoretic devices by laser engraving

Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ NMR spectroscopy

Thermal mixing of two miscible fluids in a T-shaped microchannel

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