Wireless communication technology integration is necessary for Internet of Things (IoT)-based applications to make their data easily accessible. This study proposes a new, portable L-shaped microstrip patch antenna with enhanced gain for IoT 2.4 GHz Industrial, Scientific, and Medical (ISM) applications. The overall dimensions of the antenna are 28 mm × 21 mm × 1.6 mm (0.22λo × 0.17λo × 0.013λo, with respect to the lowest frequency). The antenna design is simply comprised of an L-shape strip line, with a full ground applied in the back side and integrated with a tiny rectangular slot. According to investigations, the developed antenna is more efficient and has a greater gain than conventional antennas. The flexibility of the antenna’s matching impedance and performance are investigated through several parametric simulations. Results indicate that the gain and efficiency can be enhanced through modifying the rectangular back slot in conjunction with fine-tuning the front L-shaped patch. The finalized antenna operates at 2.4 GHz with a 98% radiation efficiency and peak gains of 2.09 dBi (measured) and 1.95 dBi (simulated). The performance of the simulation and measurement are found to be in good agreement. Based on the performance that was achieved, the developed L-shaped antenna can be used in a variety of 2.4 GHz ISM bands and IoT application environments, especially for indoor localization estimation scenarios, such as smart offices and houses, and fourth-generation (4G) wireless communications applications due to its small size and high fractional bandwidth.