Low protein diets posttranscriptionally repress apolipoprotein B expression in rat liver

Sato, Masao; Nagao, Koji; Sakono, Masafumi; Ogawa, Hiroshi; Yamamoto, Kyosuke; Imaizumi, Kazunori

doi:10.1016/s0955-2863(96)00059-9

Cited by 10 publications

(6 citation statements)

References 28 publications

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“…First, we chemically as well as histologically demonstrated the formation of fatty liver in rats fed the lowprotein diet (28)(29)(30). Impaired liver functions (31) in rats fed a protein-restricted diet were also confirmed in the present study, as shown by the decreased level of serum albumin and increased level of serum AST activity.…”

Section: Discussionsupporting

confidence: 80%

“…Because total protein contents in diets were equalized with casein, differences between the LP-EY and LP-EYO groups were derived from differences in dietary protein compositions, mainly between egg yolk protein and casein. In fact, our previous experiment showed that rats fed a lowprotein diet composed of milk whey protein alleviated partly the development of fatty liver compared to those fed casein (30). Differences between EY and EYO were protein and water-soluble contents in egg yolk.…”

Section: Discussionmentioning

confidence: 96%

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Dietary Egg Yolk Supplementation Improves Low-Protein-Diet-Induced Fatty Liver in Rats

Erami

Tanaka

Kawamura

et al. 2016

J Nutr Sci Vitaminol

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Summary Egg yolk is an important source of nutrients and contains different bioactive substances. In the present study, we studied the benefits of egg yolk in preventing low-protein-diet-induced fatty liver in rats. Rats were fed the following diets, which were based on the AIN-76 formula, for 2 wk: an adequate-protein diet containing 20% casein (C), a lowprotein diet containing 5% casein (LP-C), a low-protein diet supplemented with 12.5% egg yolk (LP-EY), and a low-protein diet supplemented with 4.1% egg yolk oil (LP-EYO). The lowprotein diets were adjusted to contain 4.13% protein and 4.7% lipids. The LP-C diet resulted in a greater increase in the liver trigriceride (TG) and the vacuolation and a greater decrease in the serum TG and free fatty acid (FFA) than did the C diet. These deviations in the serum and liver TG, serum FFA levels and the liver histopathology were corrected in rats fed the LP-EY diet but not in those fed the LP-EYO diet. Compared to rats fed the LP-C diet, although the activities of lipogenesis-related enzymes (fatty acid synthase, glucose-6-phosphate dehydrogenase, and malic enzyme) decreased in rats fed both of the LP-EY and LP-EYO diets, the level of the microsomal TG transfer protein (MTP) increased only in rats fed the LP-EY diet. Collectively, these results suggest that dietary egg yolk supplementation decreases the LP diet-induced accumulation of TG in the liver by increasing transport of TG in the liver, and egg yolk oil alone is not sufficient enough to bring about these benefits.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 96%

Dietary Egg Yolk Supplementation Improves Low-Protein-Diet-Induced Fatty Liver in Rats

Erami

Tanaka

Kawamura

et al. 2016

J Nutr Sci Vitaminol

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“…Thus, casein increases the hepatic lipid content in rats (Ascencio et al, 2004). The levels of protein in diets, also, affect the hepatic lipid metabolism in chicks and rats (Sato et al, 1996;Adams and Davis, 2001). Their results demonstrated that feeding LP diet increases the concentration of hepatic lipids, in spite of differences of responses depending on type of protein, for example, casein, whey, and soy protein.…”

Section: Introductionmentioning

confidence: 97%

“…The effects of types and levels of protein in diets on the accumulation of hepatic lipids in chicks and rats have been well established (Sato et al, 1996;Adams and Davis, 2001;Ascencio et al, 2004). Casein, a typical animal protein, stimulates the increase in serum insulin.…”

Section: Introductionmentioning

confidence: 99%

Daidzein Modulations of Apolipoprotein B and Fatty Acid Synthase mRNA Expression in Chick Liver Vary Depending on Dietary Protein Levels

Choi¹,

Song²,

Choi³

et al. 2005

Asian Australas. J. Anim. Sci

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This study was designed to determine the effects of daidzein (DE) on hepatic lipid metabolism in chicks fed with low protein (LP) diet based on casein. In experiment 1, the male chicks were fed with one of the three levels of dietary protein containing 10.95%, 21.9% and 43.8% protein content for 2 days. In experiment 2, the chicks were fed one of the three levels of protein with or without DE at 1,000 mg/kg diet for 2 days. Experiment 3 was conducted to compare DE (LP+DE) with estradiol (LP+E2) in chicks fed with LP diet for 7 days. Plasma lipid profiles, hepatic lipid profiles, activities of hepatic malic enzyme and isocitrate dehydrogenase (ICDH) were measured. Transcriptions of hepatic fatty acid synthase, apolipoprotein-B (APO-B), and fructose bisphosphatase mRNA were measured by RT-PCR. Increasing dietary protein levels markedly decreased the concentrations of plasma triglycerides, hepatic total lipids, hepatic TG, and the mRNA transcriptions while the increased dietary protein levels increased hepatic ICDH activities in experiment 1. In experiment 2, the effects of dietary protein levels on blood and hepatic lipid content were more prominent than those of the additional DE. Interestingly, plasma TG levels were affected by DE supplementation (p<0.05). In experiment 3, DE inhibited APO-B mRNA expressions and stimulated the accumulation of lipid in the liver through mechanisms different from E2. In this study, we demonstrate that DE has beneficial effects on blood lipid profiles, but that it inhibits APO-B mRNA transcription and aggravates the fatty liver induced by LP diet in chicks.

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“…Northern blotting for total cellular RNA, SDS W PAGE for reduced microsomal protein samples, and blotting the protein onto an Immun-Blot PVDF membrane (Biorad Laboratories, CA, USA) were described elsewhere. 4) The membrane was tested with the puriˆed antibody to D6-desaturase at 1:1000 dilution. Rabbit antibodies were stained with alkalinephosphatase-conjugated goat anti-rabbit IgG (H＋L) (double staining grade, Zymed Laboratories, CA, USA).…”

mentioning

confidence: 99%

Preparation of Antiserum against RatΔ6-Desaturase and Its Use to Evaluate the Desaturase Protein Levels in Rats Treated with Gemfibrozil, a Ligand for Peroxisome Proliferator-…

Shoji

Sanekata

Sato

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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Low protein diets posttranscriptionally repress apolipoprotein B expression in rat liver

Cited by 10 publications

References 28 publications

Dietary Egg Yolk Supplementation Improves Low-Protein-Diet-Induced Fatty Liver in Rats

Dietary Egg Yolk Supplementation Improves Low-Protein-Diet-Induced Fatty Liver in Rats

Daidzein Modulations of Apolipoprotein B and Fatty Acid Synthase mRNA Expression in Chick Liver Vary Depending on Dietary Protein Levels

Preparation of Antiserum against RatΔ6-Desaturase and Its Use to Evaluate the Desaturase Protein Levels in Rats Treated with Gemfibrozil, a Ligand for Peroxisome Proliferator-…

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