Low-protein/high-carbohydrate diet induces AMPK-dependent canonical and non-canonical thermogenesis in subcutaneous adipose tissue

Aquilano, Katia; Sciarretta, Francesca; Turchi, Riccardo; Li, Bo Han; Rosina, Marco; Ceci, Veronica; Guidobaldi, Giulio; Arena, Simona; D’Ambrosio, Chiara; Audano, Matteo; Salvatori, Illari; Colella, Barbara; Faraonio, Raffaella; Panebianco, Concetta; Pazienza, Valerio; Caruso, Donatella; Mitro, Nico; Bartolomeo, Sabrina Di; Scaloni, Andrea; Li, Jing Ya; Lettieri-Barbato, Daniele

doi:10.1016/j.redox.2020.101633

Cited by 21 publications

(13 citation statements)

References 60 publications

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“…The pathway of the AMPK-PGC1α axis is believed to function as a metabolic sensor involved in the regulation of brown or beige adipogenesis . AMPK, as the “fuel switch” and “energy receptor”, is the protein kinase most sensitive to energy metabolism . Herein, our results showed that the expressions of AMPK and PGC1α proteins of iBAT in HFD-fed mice were significantly elevated after the treatment with 800 mg/kg TB ( p < 0.05), where the AMPK-PGC1α signaling pathway was also activated by TB in the iWAT of the tested mice, consistent with the results of iBAT.…”

Section: Discussionmentioning

confidence: 59%

Theabrownin from Fu Brick Tea Exhibits the Thermogenic Function of Adipocytes in High-Fat-Diet-Induced Obesity

Wang

Zhao

et al. 2021

J. Agric. Food Chem.

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This study explored whether the antiobesity effect of theabrownin (TB) extracted from Fu brick tea (FBT) was associated with the activation of brown adipose tissue (BAT) or browning of the white adipose tissue (WAT) in mice fed a high-fat diet (HFD). Mice were divided into five groups, which received a normal diet, HFD, or HFD plus TB (200, 400, and 800 mg/kg), respectively. A 12-week administration of TB in a dose-dependent manner reduced the body weight and WAT weight and improved lipid and glucose disorders in the HFD-fed mice (p < 0.05). TB also promoted the expression of thermogenic and mitochondrial genes, whereas inflammation genes were reduced in interscapular BAT (iBAT), inguinal WAT (iWAT), and epididymis white adipose tissue (eWAT), accompanied by improvement in the intestinal homeostasis by improving SCFAs, especially butyric acid levels (p < 0.05), which was related to thermogenic and inflammatory factors of iBAT and iWAT. Mechanistically, TB was shown to efficiently promote thermogenesis by stimulating the AMPK-PGC1α pathway with an increase in uncoupling protein 1 (UCP1). Conclusively, these findings suggest that long-term consumption of TB can enhance BAT activity and WAT browning by activating the AMPK-PGC1α pathway and modulating SCFAs; meanwhile, SCFAs regulating TB improved inflammatory disorder in HFD-fed mice.

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Section: Discussionmentioning

confidence: 59%

Theabrownin from Fu Brick Tea Exhibits the Thermogenic Function of Adipocytes in High-Fat-Diet-Induced Obesity

Wang

Zhao

et al. 2021

J. Agric. Food Chem.

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“…Estrogen can also modulate BAT development and thermogenesis via the hypothalamic adenosine monophosphate-activated protein kinase (AMPK) signaling pathway 49 . AMPK activation can stimulate Ca 2+ cycling thermogenesis 50 . We observed significantly increased lactotransferrin (TRFL), which decreases the activity of the AMPK complex 51 .…”

Section: Discussionmentioning

confidence: 99%

Ovariectomy Interferes with Proteomes of Brown Adipose Tissue in Rats

Chou¹,

Lu²,

Liao³

et al. 2022

Int. J. Med. Sci.

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Postmenopausal women exhibit a higher prevalence of obesity due to decreased energy expenditure and increased food intake compared to their premenopausal counterparts. Brown adipose tissue (BAT) plays a key role in energy homeostasis, thus providing us with appealing therapeutic targets in obesity. However, how BAT proteomes are altered in response to low levels of estrogen remains unclear. To better understand the underlying mechanisms between the postmenopausal state and BAT proteomic changes, our study aimed to investigate the effect of ovariectomy on the BAT proteome. In this study, eight-week-old female Sprague Dawley rats were randomly allocated into bilateral ovariectomy (Ovx) and sham operation (Sham) groups. Mass spectrometry was used for proteomics assay and Ingenuity Pathway Analysis was applied to examine the differentially regulated proteins. Of the 1,412 identified proteins, 18 proteins were significantly upregulated, whereas 36 proteins were significantly downregulated in the Ovx group as compared to the Sham group. Our findings demonstrate that the proteins involved in BAT morphology, the browning of white adipose tissue, and metabolic substrates for thermogenesis were regulated by ovariectomy. The dysregulation of proteins by ovariectomy might be related to the disruption of BAT function in the postmenopausal status. Understanding how BAT proteomes are altered in response to ovariectomy may illuminate novel therapeutic strategies for the management of postmenopausal weight gain in the future.

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“…AMPK α is well known as an energy sensor in eucaryotic cells and plays critical roles in controlling systemic metabolic homeostasis [ 52 , 53 ]. However, emerging studies have revealed that AMPK α is also implicated in regulating oxidative stress and inflammation.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-1224-5p Aggravates Sepsis-Related Acute Lung Injury in Mice

Liu

Chen

Cheng

et al. 2022

Oxidative Medicine and Cellular Longevity

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Oxidative stress and inflammation are implicated in the development of sepsis-related acute lung injury (ALI). MicroRNA-1224-5p (miR-1224-5p) plays critical roles in regulating inflammatory response and reactive oxygen species (ROS) production. The present study is aimed at investigating the role and underlying mechanisms of miR-1224-5p in sepsis-related ALI. Mice were intratracheally injected with lipopolysaccharide (LPS, 5 mg/kg) for 12 h to induce sepsis-related ALI. To manipulate miR-1224-5p level, mice were intravenously injected with the agomir, antagomir, or matched controls for 3 consecutive days. Murine peritoneal macrophages were stimulated with LPS (100 ng/mL) for 6 h to further validate the role of miR-1224-5p in vitro. To inhibit adenosine 5 ′ -monophosphate-activated protein kinase alpha (AMPKα) or peroxisome proliferator activated receptor-gamma (PPAR-γ), compound C or GW9662 was used in vivo and in vitro. We found that miR-1224-5p levels in lungs were elevated by LPS injection, and that the miR-1224-5p antagomir significantly alleviated LPS-induced inflammation, oxidative stress, and ALI in mice. Conversely, the miR-1224-5p agomir aggravated inflammatory response, ROS generation, and pulmonary dysfunction in LPS-treated mice. In addition, the miR-1224-5p antagomir reduced, while the miR-1224-5p agomir aggravated LPS-induced inflammation and oxidative stress in murine peritoneal macrophages. Further findings revealed that miR-1224-5p is directly bound to the 3 ′ -untranslated regions of PPAR-γ and subsequently suppressed PPAR-γ/AMPKα axis, thereby aggravating LPS-induced ALI in vivo and in vitro. We demonstrate for the first time that endogenous miR-1224-5p is a critical pathogenic factor for inflammation and oxidative damage during LPS-induced ALI through inactivating PPAR-γ/AMPKα axis. Targeting miR-1224-5p may help to develop novel approaches to treat sepsis-related ALI.

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Low-protein/high-carbohydrate diet induces AMPK-dependent canonical and non-canonical thermogenesis in subcutaneous adipose tissue

Cited by 21 publications

References 60 publications

Theabrownin from Fu Brick Tea Exhibits the Thermogenic Function of Adipocytes in High-Fat-Diet-Induced Obesity

Theabrownin from Fu Brick Tea Exhibits the Thermogenic Function of Adipocytes in High-Fat-Diet-Induced Obesity

Ovariectomy Interferes with Proteomes of Brown Adipose Tissue in Rats

MicroRNA-1224-5p Aggravates Sepsis-Related Acute Lung Injury in Mice

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