Low-Selenium Diet Induces Cell Cycle Arrest of Thymocytes and Alters Serum IL-2 Content in Chickens

Abstract

The purpose of this 42-day study was to investigate the effects of low selenium (Se) on cellular immune function by determining cell cycle of thymus, serum IL-2 content, and mitogenesis of peripheral blood T-lymphocytes. One hundred twenty 1-day-old Avian broilers were randomly assigned to two groups of 60 each and were fed on a low-Se diet (0.0342 mg/kg Se) or a control diet (0.2 mg/kg Se), respectively. Cell cycle analysis by flow cytometry showed that low-Se diet caused an increase in G(0)G(1) phase cells that corresponded to a decrease in S-phase cells in thymus. Ultrastructurally, mitochondria injury and increased apoptotic cells with condensed nuclei were observed. Low-Se diet decreased the serum IL-2 contents and mitogenesis of peripheral blood lymphocytes to concanavalin A in comparison with those of control group. These data indicate that low-Se diet inhibits the development of thymus by arresting the cell cycle and decreasing the IL-2 content.

In ovotrace element supplementation enhances expression of growth genes in embryo and immune genes in post-hatch broiler chickens

“…This is in line with earlier reports where there was increased IL-2 secretion and Th1 cytokines (IFN-and IL-12) production in internal or external selenium treated chronic hepatitis patients and selenium nanoparticles treated spleen cell culture of mice. 56,57 Peng et al 58 also reported decrease in the serum IL-2 contents of chicken fed low-Se diet. In vivo response to PHA-P in Se injected chicks were better than un-injected control chicks.…”

The WOMED model of benign thyroid disease: Acquired magnesium deficiency due to physical and psychological stressors relates to dysfunction of oxidative phosphorylation

“…This picture suggests a defect in selenium utilization or transport (unpublished data from 2007) as well as a potential defect in selenoprotein synthesis. Alterations in selenium economy can be associated with changes in the immune system [248] , [249] . In addition to this a modulation of gene expression profiles of Complex I, IV and V has been observed following administration of selenium [250] .…”

Effects of dietary selenium on host response to necrotic enteritis in young broilers

“…Previous researches showed that Se deficiency impaired the cellular and humoral immune function and Se supplementation induced favorable effects on T cell responses and antibody synthesis in chickens (Marsh et al, 1981;Peng et al, 2011aPeng et al, , 2011bPeng et al, , 2012. In the present study, broiler chickens fed with appropriate B-Traxim Se showed increased body weight gain, reduced intestinal lesions, improved serum antibody to Net-B toxin, and upregulated transcript levels of IL-1β, IL-6, IL-8, LITAF, TNFSF15, iNOS, AvBD6, AvBD8 and AvBD13 compared with the unsupplemented and infected chickens using an experimental model of NE, which was consistent with our previous study evaluating the effects of in ovo injection with Se on immune and antioxidant responses during experimental NE in commercial broilers (Lee et al, 2014).…”