Low-temperature crosslinking of proteins using non-toxic citric acid in neutral aqueous medium: Mechanism and kinetic study

Xu, Helan; Shen, Li; Xu, Lan; Yang, Yiqi

doi:10.1016/j.indcrop.2015.05.010

Cited by 82 publications

(46 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These are natural organic acids, present, in vegetables and fruits, synthesized during fermentation by microorganisms. They can be produced in large quantities, by biotechnological techniques, and can be used to modify polysaccharides . The two carboxylic acid groups can produce chemical bridges between hydroxyl groups of the polysaccharide molecules.…”

Section: Introductionmentioning

confidence: 75%

Development, modification and characterization of new biodegradable film from basil seed (Ocimum basilicum L.) mucilage

Thessrimuang

Prachayawarakorn

2019

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND: Biodegradable films from basil seed mucilage (BSM) were formed and modified with several di-carboxylic acid crosslinkers; i.e. tartaric acid (TA), malic acid (MA) and succinic acid (SA) with varying acidity and chemical structures, to enhance mechanical properties and water barrier ability. Basil seeds have a reasonable mucilage content and valuable properties; thus, it has the potential to develop valuable new biodegradable films. RESULT:We characterized BSM films with the three crosslinkers using Fourier-transform infrared (FTIR) spectra and observed a 1730 cm −1 C O stretching peak, which confirmed ester linkage between the mucilage and crosslinkers. The crosslinked films showed higher gel fraction than native films. The crosslinked films showed better swelling and water vapor permeability with SA than with TA and MA. Crosslinking led to significant improvement in strain at maximum load. Further, the stress maximum load was comparable to that of commercial low-density polyethylene (LDPE) film. Crosslinked films showed additional homogeneous morphology and an increase in thermal degradation temperatures. CONCLUSION: Crosslinking with dicarboxylic acids improved all the key properties of BSM films, including excellent stress and strain at maximum load, improved barrier capability and thermal properties. Thus, these films showed good potential as biodegradable films, especially for food packaging.

show abstract

Section: Introductionmentioning

confidence: 75%

Development, modification and characterization of new biodegradable film from basil seed (Ocimum basilicum L.) mucilage

Thessrimuang

Prachayawarakorn

2019

J Sci Food Agric

View full text Add to dashboard Cite

show abstract

“…CA is an aliphatic polyfunctional bio‐based raw material that contains 2 reactive primary carboxylic groups, one sterically hindered hydroxyl group and one less reactive tertiary carboxylic group . In this work, protein films were prepared at pH = 10 and thus, carboxylic groups in CA were most probably in the form of carboxylates, that reacts with the functional groups N‐terminal amine and forms an amide linkage, leading to the cross‐link with protein after the heating treatment . Indeed, nucleophilic substitution is the proposed mechanism for the reaction between protein and CA.…”

Section: Resultsmentioning

confidence: 99%

Rational design and characterization of bioplastics from Hermetia illucens prepupae proteins

et al. 2018

View full text Add to dashboard Cite

In this study proteins extracted from prepupae of Hermetia illucens, also known as black soldier fly, are investigated as promising base for a new type of bioplastics for agricultural purposes. Design of experiments techniques are employed to perform a rational study on the effects of different combination of glycerol as plasticizer, citric acid as cross‐linking agent and distilled water as solvent on the capability of proteins to form a free‐standing film through casting technique, keeping as fixed the quantity of proteins. Glycerol shows interesting properties as plasticizer contributing to the formation of homogenous and free‐standing film. Moreover, mechanical and thermal characterizations are performed to estimate the effect of increasing amounts of proteins on the final properties and thickness of the specimens. Proteins derived from H. illucens can be successfully employed as base for bioplastics to be employed for agricultural purposes.

show abstract

“…Such an esterification would improve the water resistance of films [35], as shown by the swelling curves in Figure 1. Furthermore, since the films were prepared at basic pH, the nucleophilic substitution between carboxylate groups in citric acid and amine groups in gelatin was favored [36].…”

Section: Physicochemical Propertiesmentioning

confidence: 99%

The Effect of Cross-Linking with Citric Acid on the Properties of Agar/Fish Gelatin Films

et al. 2020

View full text Add to dashboard Cite

The aim of this work was to assess the effect of fish gelatin–citric acid nucleophilic substitution and agar–citric acid esterification reactions on the properties of agar/fish gelatin films. Since temperature is an important cross-linking parameter, films were treated at 90 °C and 105 °C and film properties were compared to those of non-cured films. It was observed that temperature favored the aforementioned reactions, which induced physical and morphological changes. In this regard, darker films with a rougher surface were obtained for the films with a higher cross-linking degree. While mechanical properties were slightly modified, the barrier properties were enhanced due to the reactions that occurred. Therefore, these agar/fish gelatin films cross-linked through two different reactions can be considered to be promising materials as active films for different purposes, such as active packaging or pharmaceutical applications.

show abstract

Low-temperature crosslinking of proteins using non-toxic citric acid in neutral aqueous medium: Mechanism and kinetic study

Cited by 82 publications

References 34 publications

Development, modification and characterization of new biodegradable film from basil seed (Ocimum basilicum L.) mucilage

Development, modification and characterization of new biodegradable film from basil seed (Ocimum basilicum L.) mucilage

Rational design and characterization of bioplastics from Hermetia illucens prepupae proteins

The Effect of Cross-Linking with Citric Acid on the Properties of Agar/Fish Gelatin Films

Contact Info

Product

Resources

About