Low-Temperature Induced Transmembrane Potential Changes in the Liverwort Conocephalum conicum

Król, Elżbieta; Dziubińska, Halina; Trębacz, Kazimierz

doi:10.1093/pcp/pcg070

Cited by 46 publications

(58 citation statements)

References 35 publications

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“…4, C and I). Such dose-dependent voltage transients may be accompanied by a transient rise in Ca 2+ permeability (Krol and Trebacz, 1999;Krol et al, 2003Krol et al, , 2004 but may not recruit sufficient Ca 2+ from internal stores to trigger a forisome response Hafke and van Bel, 2013).…”

Section: Discussion Ses Contain a Peripheral Mictoplasmic Actin Meshworkmentioning

confidence: 99%

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Involvement of the Sieve Element Cytoskeleton in Electrical Responses to Cold Shocks

Hafke¹,

Ehlers

Föller

et al. 2013

Plant Physiology

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This study dealt with the visualization of the sieve element (SE) cytoskeleton and its involvement in electrical responses to local cold shocks, exemplifying the role of the cytoskeleton in Ca 2+ -triggered signal cascades in SEs. High-affinity fluorescent phalloidin as well as immunocytochemistry using anti-actin antibodies demonstrated a fully developed parietal actin meshwork in SEs. The involvement of the cytoskeleton in electrical responses and forisome conformation changes as indicators of Ca 2+ influx was investigated by the application of cold shocks in the presence of diverse actin disruptors (latrunculin A and cytochalasin D). Under control conditions, cold shocks elicited a graded initial voltage transient, DV 1 , reduced by external La 3+ in keeping with the involvement of Ca 2+ channels, and a second voltage transient, DV 2 . Cytochalasin D had no effect on DV 1 , while DV 1 was significantly reduced with 500 nM latrunculin A. Forisome dispersion was triggered by cold shocks of 4°C or greater, which was indicative of an all-or-none behavior. Forisome dispersion was suppressed by incubation with latrunculin A. In conclusion, the cytoskeleton controls cold shock-induced Ca 2+ influx into SEs, leading to forisome dispersion and sieve plate occlusion in fava bean (Vicia faba).

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Section: Discussion Ses Contain a Peripheral Mictoplasmic Actin Meshworkmentioning

confidence: 99%

“…therein). Repolarization depends on K + -outward channels (Trebacz et al, 1994;Krol et al, 2003;Supplemental Fig. S2) identified at the plasma membrane of isolated SE protoplasts .…”

Section: Discussion Ses Contain a Peripheral Mictoplasmic Actin Meshworkmentioning

confidence: 99%

Involvement of the Sieve Element Cytoskeleton in Electrical Responses to Cold Shocks

Hafke¹,

Ehlers

Föller

et al. 2013

Plant Physiology

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“…Early studies identified cold-induced potential changes (CIPCs) in Cucumis, Beta, and Glycine roots, Avena and Hordeum coleoptiles, Lonicera and Hedera leaves, Limnobium root hairs, and Allium epidermal cells (Minorsky, 1989). Later, a large and transient depolarization was recorded in the moss Conocephalum conicum (Krol et al, 2003) and in mesophyll cells of Arabidopsis (Arabidopsis thaliana), Helianthus annuus, and Vicia faba (Krol et al, 2004). Recently, in Arabidopsis plants expressing the calcium reporter apoaequorin in the cytoplasm (Knight et al, 1996;Plieth et al, 1999;Knight, 2002), it was found that a temperature drop of several degrees caused an immediate and transient rise in cytosolic calcium.…”

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confidence: 99%

Cold Transiently Activates Calcium-Permeable Channels in Arabidopsis Mesophyll Cells

et al. 2006

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Living organisms are capable of discriminating thermal stimuli from noxious cold to noxious heat. For more than 30 years, it has been known that plant cells respond to cold with a large and transient depolarization. Recently, using transgenic Arabidopsis (Arabidopsis thaliana) expressing the calcium-sensitive protein aequorin, an increase in cytosolic calcium following cold treatment was observed. Applying the patch-clamp technique to Arabidopsis mesophyll protoplasts, we could identify a transient plasma membrane conductance induced by rapid cooling. This cold-induced transient conductance was characterized as an outward rectifying 33 pS nonselective cation channel. The permeability ratio between calcium and cesium was 0.7, pointing to a permeation pore .3.34 Å (ø of cesium). Our experiments thus provide direct evidence for the predicted but not yet measured cold-activated calcium-permeable channel in plants.

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“…Simultaneously, there are reports about intracellular investigations conducted on another liverwort Conocephalum conicum (Dziubińska et al 1983;Favre et al 1999b;Król et al 2007;Zawadzki and Trębacz 1985) and on the moss Physcomitrella patens, a species that is phylogenetically related to liverworts and regarded as a model system in plant functional genomics (Decker et al 2006;Reski 1999) and electrophysiology (Ermolayeva et al 1996(Ermolayeva et al , 1997Koselski et al 2008Koselski et al , 2013Koselski et al , 2015. Conocephalum conicum displays excitability to various stimuli, such as light (Król and Trębacz 1999;Trębacz and Zawadzki 1985;Trębacz et al 1989a), cold (Król et al 2003;Kupisz and Trebacz 2011), wounding (Favre et al 1999a), and direct current (DC) (Dziubińska et al 1983;Król and Trębacz 1999;Paszewski et al 1982). Among the stimuli mentioned above, light signals are considered as environmental factors that play a critical role in growth, reproduction, development, and regeneration, distinguishing liverworts, mosses, and other bryophytes from other plant species (Cove and Ashton 1988).…”

Section: Introductionmentioning

confidence: 99%

Generation of action potential-type changes in response to darkening and illumination as indication of the plasma membrane proton pump status in Marchantia polymorpha

2017

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The aim of the present study was to characterise bioelectrical changes in the membrane potential of Marchantia polymorpha gametophyte cells after light/dark transitions and to determine the role of the proton pump and energy status of the M. polymorpha cells in generation of these changes. Darkening caused persistent depolarisation of the resting potential (RP) and generation of shortlasting potential changes that were not uniform among different thalli. In some plants (18%), the changes evoked by darkening were typical action potentials (APs dark ), whereas in 69% of the plants, the changes had a form of action potential-like responses (APs dark -like) consisting of a transient depolarisation followed by a plateau phase, whose magnitude and duration were inconstant. The illumination of the M. polymorpha always evoked action potentials (APs light ) if the thallus was illuminated with light intensity of at least 120 lmol photons m -2 s -1 after 30-min darkening. To analyse the involvement of H ? -ATPase in formation of the illumination/darkening-induced electrical responses in M. polymorpha, the proton pump regulators were used. The proton pump inhibitor (20 lM FCCP) significantly diminished the RP and inhibited darkinduced AP dark and/or AP dark -like responses and illumination-induced APs light . After application of DCMU (20 lM), the RP was strongly depolarised and no response to light/dark was observed. Fusicoccin (20 lM), i.e., an activator of the proton pump, strongly hyperpolarised the membrane potential and blocked dark-induced AP dark / AP dark -like responses and illumination-induced APs light .

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Low-Temperature Induced Transmembrane Potential Changes in the Liverwort Conocephalum conicum

Cited by 46 publications

References 35 publications

Involvement of the Sieve Element Cytoskeleton in Electrical Responses to Cold Shocks

Involvement of the Sieve Element Cytoskeleton in Electrical Responses to Cold Shocks

Cold Transiently Activates Calcium-Permeable Channels in Arabidopsis Mesophyll Cells

Generation of action potential-type changes in response to darkening and illumination as indication of the plasma membrane proton pump status in Marchantia polymorpha

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