Low‐temperature plasma‐activated medium inhibited invasion and metastasis of melanoma cells via suppressing the Wnt/β‐catenin pathway

Liu, Jinren; Gao, Lingyan; Wu, Yueming; Xu, Genhui; Ma, Yan; Hao, Yanpeng; Xue-yin, Shi; Zhang, Guanjun

doi:10.1002/ppap.201900060

Cited by 12 publications

(4 citation statements)

References 71 publications

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“…An important parameter in indirect CAP treatment is the interaction between the PAM and the target material (i.e., HOS cells), also called treatment time. According to the specialized literature, in previous studies different treatment times were used, starting from 10 min, up to 72 h [3,9,60,75,[77][78][79][80][81][82][83][84].…”

Section: Discussionmentioning

confidence: 99%

“…However, Mateu-Sanz et al and Liu et al were able to produce PAM without any alterations to the pH levels. The main difference in their approach was the addition of FBS to the solution that was exposed to CAP, either before or after the exposure [9,81]. According to Subramanian et al, the pH reduction observed following CAP exposure is a result of increased hydrogen ion concentration [3].…”

Section: Discussionmentioning

confidence: 99%

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Optimization of Indirect CAP Exposure as an Effective Osteosarcoma Cells Treatment with Cytotoxic Effects

et al. 2023

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Over the past decade, cold atmospheric plasma (CAP) has undergone extensive research as a promising therapeutic approach in oncology, with different treatment methods and exposure configurations being investigated and resulting in various biological effects, most of them after long exposure or treatment durations. This study aimed to evaluate the potential of a custom-made CAP generation source to produce plasma-activated medium (PAM) with cytotoxic effects and subsequently to establish the optimal exposure and treatment parameters. The exposure’s electrical parameters, as well as pH and NO2− content of PAM were analyzed. The cytotoxic potential and optimal parameters of the treatment were established by evaluating the viability of human osteosarcoma cells (HOS cell line) and human osteoblasts (HOB cell line) treated with PAM under different conditions. Our results showed that indirect treatment with CAP presents selective dose-dependent cytotoxic effects, while the cell viability decrease was not found to be correlated with the PAM acidification due to CAP exposure. The Griess assay revealed very high and long-term stable concentrations of NO2− in PAM. Overall, this study presents a simple and faster method of producing PAM treatment with cytotoxic effects on HOS cells, by using a custom-built CAP source.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Optimization of Indirect CAP Exposure as an Effective Osteosarcoma Cells Treatment with Cytotoxic Effects

et al. 2023

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“…Recently, PAM has been widely studied for antitumor research, proving that its production of a large number of ROS was the main factor in cancer cell apoptosis [22,25,26]. In addition, PAM has been found to inhibit the migration of melanoma cells [27], but whether it inhibits the migration of NSCLC and its mechanisms has not been explored.…”

Section: Introductionmentioning

confidence: 99%

Low-Temperature Plasma-Activated Medium Inhibits the Migration of Non-Small Cell Lung Cancer Cells via the Wnt/β-Catenin Pathway

Zhang

Yan

et al. 2023

Biomolecules

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This study explored the molecular mechanism of the plasma activation medium (PAM) inhibiting the migration ability of NSCLC (non-small cell lung cancer) cells. The effect of PAM incubation on the cell viability of NSCLC was detected through a cell viability experiment. Transwell cells and microfluidic chips were used to investigate the effects of PAM on the migration capacity of NSCLC cells, and the latter was used for the first time to observe the changes in the migration capacity of cancer cells treated with PAM. Moreover, the molecular mechanisms of PAM affecting the migration ability of NSCLC cells were investigated through intracellular and extracellular ROS detection, mitochondrial membrane potential, and Western blot experiments. The results showed that after long-term treatment with PAM, the high level of ROS produced by PAM reduced the level of the mitochondrial membrane potential of cells and blocked the cell division cycle in the G2/M phase. At the same time, the EMT process was reversed by inhibiting the Wnt/β-catenin signaling pathway. These results suggested that the high ROS levels generated by the PAM treatment reversed the EMT process by inhibiting the WNT/β-catenin pathway in NSCLC cells and thus inhibited the migration of NSCLC cells. Therefore, these results provide good theoretical support for the clinical treatment of NSCLC with PAM.

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“…PAM can increase the level of oxidative stress in cells and thus promote or inhibit the proliferation of cells. Related studies have shown that short-term PAM treatment can promote the proliferation of fibroblasts [3], induce osteoblast differentiation [4], inhibit over 20 kinds of cancer cells cultured in vitro [5], including melanoma cells [6], prostate cancer cells [7], glioma cells [8], lymphoma cells [9], and liver cancer cells [10]. The main mechanisms of action include inducing apoptosis and necrosis of tumor cells, inhibiting proliferation signaling pathway, damaging DNA structure, and affecting migration and adhesion of tumor cells [11].…”

Section: Introductionmentioning

confidence: 99%

Effects and Mechanism of Plasma-Activated Medium on Angiogenesis of Vascular Endothelial Cells

Hao

Wang

et al. 2021

Applied Sciences

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After cell culture medium is treated with low temperature plasma (LTP), the liquid is rich in reactive oxygen and nitrogen species (RONS), and becomes plasma-activated medium (PAM). PAM, as the supplier of RONS, can affect the angiogenesis of cells. The purpose of this study is to investigate the effects and related mechanism of PAM on human umbilical vein endothelial cells (HUVECs). Cell viability and cell cycle were evaluated after HUVECs were treated with PAM for 24 h. Changes in cell angiogenesis, migration and adhesion, secretion of cytokines such as VEGF and bFGF, expression of VEGFR-2 and phosphorylation of the key proteins in the MEK/ERK signaling pathway, concentrations of H2O2 and NO2− in PAM and in cells were also investigated. The results showed that PAM obtained by LTP treatment had dual effects on the angiogenesis of HUVECs: PAM obtained by short-term LTP treatment promoted the angiogenesis of HUVECs, while PAM obtained by long-term LTP treatment inhibited the angiogenesis of HUVECs. The mechanism may be that PAM treatment changes the content of RONS, affects the VEGF-VEGFR-2 signaling pathway, and ultimately affects the angiogenesis of HUVECs.

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Low‐temperature plasma‐activated medium inhibited invasion and metastasis of melanoma cells via suppressing the Wnt/β‐catenin pathway

Cited by 12 publications

References 71 publications

Optimization of Indirect CAP Exposure as an Effective Osteosarcoma Cells Treatment with Cytotoxic Effects

Optimization of Indirect CAP Exposure as an Effective Osteosarcoma Cells Treatment with Cytotoxic Effects

Low-Temperature Plasma-Activated Medium Inhibits the Migration of Non-Small Cell Lung Cancer Cells via the Wnt/β-Catenin Pathway

Effects and Mechanism of Plasma-Activated Medium on Angiogenesis of Vascular Endothelial Cells

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