Low temperature pretreatment of sugarcane bagasse at atmospheric pressure using mixtures of ethylene carbonate and ethylene glycol

Zhang, Zhanying; O’Hara, Ian M.; Rackemann, Darryn W.; Doherty, William O.S.

doi:10.1039/c2gc36323b

Cited by 31 publications

(59 citation statements)

References 56 publications

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“…As previously reported the glucan digestibility and glucose yield of bagasse pretreated with the mixture of EC/EG was much higher than bagasse pretreated with EC alone and also significantly higher than bagasse pretreated with EG alone [10]. Repeated experiments with EC/EG solvents in this study confirmed our previous observation (Table 1).…”

Section: Resultssupporting

confidence: 91%

“…XRD analysis also showed that the pretreatments did not decrease cellulose crystallinity (Additional file 1: Figure S1) and the estimated CrIs of the pretreated biomass (0.73 - 0.75) were slightly higher than that of untreated bagasse (0.68). Slight increase in CrI was also observed in our previous studies where acidified solvents were used to pretreat sugarcane bagasse due to the removal of amorphous components [10,31]. Figure 2 also shows that the peak at 1050 cm -1 , which is associated with the C-O stretch in cellulose and hemicellulose [28], was prominent in pretreated bagasse, indicating the increase in glucan content.…”

Section: Resultssupporting

confidence: 72%

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Glycerol carbonate as green solvent for pretreatment of sugarcane bagasse

Zhang

Rackemann

Doherty

et al. 2013

Biotechnol Biofuels

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BackgroundPretreatment of lignocellulosic biomass is a prerequisite for effective saccharification to produce fermentable sugars. In this study, “green” solvent systems based on acidified mixtures of glycerol carbonate (GC) and glycerol were used to treat sugarcane bagasse and the roles of each solvent in deconstructing biomass were determined.ResultsPretreatment of sugarcane bagasse at 90°C for only 30 min with acidified GC produced a solid residue having a glucan digestibility of 90% and a glucose yield of 80%, which were significantly higher than a glucan digestibility of 16% and a glucose yield of 15% obtained for bagasse pretreated with acidified ethylene carbonate (EC). Biomass compositional analyses showed that GC pretreatment removed more lignin than EC pretreatment (84% vs 54%). Scanning electron microscopy (SEM) showed that fluffy and size-reduced fibres were produced from GC pretreatment whereas EC pretreatment produced compact particles of reduced size. The maximal glucan digestibility and glucose yield of GC/glycerol systems were about 7% lower than those of EC/ethylene glycol (EG) systems. Replacing up to 50 wt% of GC with glycerol did not negatively affect glucan digestibility and glucose yield. The results from pretreatment of microcrystalline cellulose (MCC) showed that (1) pretreatment with acidified alkylene glycol (AG) alone increased enzymatic digestibility compared to pretreatments with acidified alkylene carbonate (AC) alone and acidified mixtures of AC and AG, (2) pretreatment with acidified GC alone slightly increased, but with acidified EC alone significantly decreased, enzymatic digestibility compared to untreated MCC, and (3) there was a good positive linear correlation of enzymatic digestibility of treated and untreated MCC samples with congo red (CR) adsorption capacity.ConclusionsAcidified GC alone was a more effective solvent for pretreatment of sugarcane bagasse than acidified EC alone. The higher glucose yield obtained with GC-pretreated bagasse is possibly due to the presence of one hydroxyl group in the GC molecular structure, resulting in more significant biomass delignification and defibrillation, though both solvent pretreatments reduced bagasse particles to a similar extent. The maximum glucan digestibility of GC/glycerol systems was less than that of EC/EG systems, which is likely attributed to glycerol being less effective than EG in biomass delignification and defibrillation. Acidified AC/AG solvent systems were more effective for pretreatment of lignin-containing biomass than MCC.

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 72%

Glycerol carbonate as green solvent for pretreatment of sugarcane bagasse

Zhang

Rackemann

Doherty

et al. 2013

Biotechnol Biofuels

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“…The compositions of untreated bagasse and bagasse pretreated in the Mackay Renewable Biocommodities Pilot Plant with dilute H 2 SO 4 steam explosion, dilute NaOH steam explosion, and glycerol/HCl steam explosion under conditions described in our previous study [23] are presented in Table 1. The compositions of untreated (depithed) and bagasse pretreated at the laboratory scale with acidified EC/EG [38], BMIMCl [39], and ballmilling are presented in the same table. Percentages of glucan (cellulose), xylan (the majority of hemicellulose), lignin, and ash were calculated on a dry mass basis.…”

Section: Pretreatment and Characterisation Of Bagassementioning

confidence: 99%

“…To expand the available range of pretreated sugar cane bagasse, air-dried and depithed sugar cane bagasse was pretreated at the laboratory scale using a mixture of EC and EG, an ionic liquid (BMIMCl), or a purely physical pretreatment (ball-milling). The EC/EG pretreatment was undertaken at a solidto-liquid ratio of 4:1 (w/w) in the presence of 1.2% (w/w) H 2 SO 4 at 90°C for 30 min using the method described by Zhang et al (2013) [38]. The ionic liquid pretreatment was undertaken with BMIMCl containing 0.7% (w/w) water at a 10:1 (w/w) liquid-to-solid ratio at 150°C for 1 h using the method described by Karatzos et al (2012) [39].…”

Section: Pretreatment Of Sugar Cane Bagassementioning

confidence: 99%

The combination of plant-expressed cellobiohydrolase and low dosages of cellulases for the hydrolysis of sugar cane bagasse

Harrison

Zhang

Shand

et al. 2014

Biotechnol Biofuels

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Background: The expression of biomass-degrading enzymes (such as cellobiohydrolases) in transgenic plants has the potential to reduce the costs of biomass saccharification by providing a source of enzymes to supplement commercial cellulase mixtures. Cellobiohydrolases are the main enzymes in commercial cellulase mixtures. In the present study, a cellobiohydrolase was expressed in transgenic corn stover leaf and assessed as an additive for two commercial cellulase mixtures for the saccharification of pretreated sugar cane bagasse obtained by different processes. Results: Recombinant cellobiohydrolase in the senescent leaves of transgenic corn was extracted using a simple buffer with no concentration step. The extract significantly enhanced the performance of Celluclast 1.5 L (a commercial cellulase mixture) by up to fourfold on sugar cane bagasse pretreated at the pilot scale using a dilute sulfuric acid steam explosion process compared to the commercial cellulase mixture on its own. Also, the extracts were able to enhance the performance of Cellic CTec2 (a commercial cellulase mixture) up to fourfold on a range of residues from sugar cane bagasse pretreated at the laboratory (using acidified ethylene carbonate/ethylene glycol, 1-butyl-3-methylimidazolium chloride, and ball-milling) and pilot (dilute sodium hydroxide and glycerol/hydrochloric acid steam explosion) scales. We have demonstrated using tap water as a solvent (under conditions that mimic an industrial process) extraction of about 90% recombinant cellobiohydrolase from senescent, transgenic corn stover leaf that had minimal tissue disruption.

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“…The pretreatment processes for delignification more frequently used are acid hydrolysis, hydrothermal pretreatment, alkaline pretreatment microwave assisted, low-temperature pretreatment using mixtures of ethylene carbonate and ethylene glycol [17], assisted by cavitation [18] [19], mixtures of glycerol water [20], ethanol-water mixtures using carbon dioxide at high pressures [21], subsequent extraction with organic solvents at acidic pretreatment [11], hydrodynamic cavitation, microwave assisted extraction [22] [23] between others [14] [24].…”

Section: Introductionmentioning

confidence: 99%

Ethyl Levulinate Obtained from Lignocellulosic Waste Material with Previous Delignification by Ultrasonic-Assisted Technique

Solá-Pérez¹,

Saldarriaga-Noreña²,

Murillo-Tovar³

2017

JACEN

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Ultrasound-assisted pretreatment under mild operating conditions has been investigated for intensification of delignification to facilitate the obtaining of ethyl levulinate from biomass. The effect of pH (2 -12), temperature (30˚C -70˚C) and pretreatment time (0 -120 minutes) has been studied for different biomass samples. The most favorable conditions were basic pH, temperature of 70˚C and pretreatment time of 2 h, obtaining values of delignification near 80 percent. The ethyl levulinate is obtained in microwave directly via from samples before and after delignification and analyzed for GC-MS. The results evidenced better yields for the delignified samples.

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Low temperature pretreatment of sugarcane bagasse at atmospheric pressure using mixtures of ethylene carbonate and ethylene glycol

Cited by 31 publications

References 56 publications

Glycerol carbonate as green solvent for pretreatment of sugarcane bagasse

Glycerol carbonate as green solvent for pretreatment of sugarcane bagasse

The combination of plant-expressed cellobiohydrolase and low dosages of cellulases for the hydrolysis of sugar cane bagasse

Ethyl Levulinate Obtained from Lignocellulosic Waste Material with Previous Delignification by Ultrasonic-Assisted Technique

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