Low-Temperature Scanning Electron Microscopy

Beckett, A.; Read, Nick D.

doi:10.1007/978-1-4684-5119-1_2

Cited by 67 publications

(46 citation statements)

References 42 publications

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“…The use of LTSEM enables samples to be frozen, coated and observed rapidly without the use of liquid fixatives (Beckett & Read, 1986). Using this technique, it was apparent that algal and fungal cells in P. sulcata thalli stored at 100% RH were fully inflated [ Fig.…”

Section: Low Temperature Scanning Electron Microscopymentioning

confidence: 99%

THE EFFECT OF DESICCATION ON CELL SHAPE IN THE LICHEN PARMELIA SULCATA TAYLOR

et al. 1987

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SUMMARYComparisons have been made of the shape of algal cells in the lichen Parmelia sulcata which was subjected to controlled desiccation regimes inducing substantial water loss. The spherical appearance of algal cells obtained by conventional techniques for transmission electron microscopy (TEM) was shown by low temperature scanning electron microscopy (LTSEM) to be the consequence of rapid rehydration during fixation. Collapse of the walls of algal cells and fungal hyphae in the medulla and algal layer when desiccated were observed with LTSEM and shown to be reversible on rehydration. Desiccation-induced contraction of cortical fungal protoplasts was detected with LTSEM. Fixation with osmium vapour only before TEM demonstrated a similar contraction of algal protoplasts.

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Section: Low Temperature Scanning Electron Microscopymentioning

confidence: 99%

THE EFFECT OF DESICCATION ON CELL SHAPE IN THE LICHEN PARMELIA SULCATA TAYLOR

et al. 1987

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“…Second, cryofixation preserves the natural turgid structure of the mite so that important taxonomic features can be observed. Critical point drying, air drying, or drying from an organic solvent are all associated with distortion or collapse of biological samples (Beckett and Read 1986). No indications of these artifacts were observed in the present study.…”

Section: Discussionmentioning

confidence: 46%

“…However, before the mites are imaged in a conventional SEM, they are typically chemically fixed, dehydrated, and/or thoroughly dried. Unfortunately, these preparation methods are generally associated with artifacts in biological specimens (see review, Beckett and Read 1986), including mites (Alberti andNuzzaci 1996, Ebrahim et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Use of low‐temperature field emission scanning electron microscopy to examine mites

et al. 2000

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Summary:Partly because mites are microscopic in size and fragile in nature, acarologists estimate that less than five percent of extant species have been taxonomically described. Recently, data from conventional scanning electron microscopy (SEM) have been used to facilitate the descriptions and complement the information that has been historically obtained with the light microscope. However, the conventional preparation techniques associated with SEM frequently prevent or compromise the results. This study evaluated the use of low-temperature field emission SEM to image mites and their hosts. Results indicated that a modified cryofixation procedure, which was associated with this technique, retained the mites at their living/feeding sites in natural behavioral positions. Furthermore, the turgor of the specimens, even eggs and soft-bodied species, was also maintained. The structure and orientation of delicate structures such as setae, which would be subjected to mechanical damage during conventional chemical fixation, dehydration, and drying, were also preserved after cryofixation. Field emission SEM, which provided useful magnification beyond that attainable with a conventional SEM, also enabled resolution of ultrastructural features, such as tenent hairs on the empodium and pores on the dorsal surface that had not previously been observed. These advantages indicate that the low-temperature field emission SEM can provide important structural data that can be used to study the anatomy, morphology, and bioecology of mites.

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“…Other imaging modalities are cryo-FE-SEM and TEM immuno-techniques (Daniel et al, 2004). Especially low temperature SEM is a valuable tool (Refshauge et al, 2006) to examine samples with minimum disruption to structural integrity by rapidly freezing the specimen to temperatures below -130°C in which state the specimen is considered to be in a fully frozen hydrated condition (Beckett & Read 1986;Abu Ali et al, 1999). At last, X-ray analysis is a promising technique for mycological research.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional X-Ray Imaging and Analysis of Fungi on and in Wood

et al. 2009

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As wood is prone to fungal degradation, fundamental research is necessary to increase our knowledge aiming at product improvement. Several imaging modalities are capable of visualizing fungi, but the X-ray equipment presented in this paper can envisage fungal mycelium in wood non-destructively in three dimensions with sub-micron resolution. Four types of wood subjected to the action of the white rot fungus Coriolus versicolor (Linnaeus) Quélet (CTB 863 A) were scanned using an X-ray based approach. Comparison of wood volumes before and after fungal exposure, segmented manually or semi-automatically, showed the presence of the fungal mass on and in the wood samples and therefore demonstrated the usefulness of computed X-ray tomography for mycological and wood research. Further improvements to the experimental set-up are necessary in order to resolve individual hyphae and enhance segmentation.2

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Low-Temperature Scanning Electron Microscopy

Cited by 67 publications

References 42 publications

THE EFFECT OF DESICCATION ON CELL SHAPE IN THE LICHEN PARMELIA SULCATA TAYLOR

THE EFFECT OF DESICCATION ON CELL SHAPE IN THE LICHEN PARMELIA SULCATA TAYLOR

Use of low‐temperature field emission scanning electron microscopy to examine mites

Three-Dimensional X-Ray Imaging and Analysis of Fungi on and in Wood

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