Low-temperature sol–gel-derived nanosilver-embedded silane coating as biofilm inhibitor

Abstract

Silver nanocomposite coatings are prepared by the sol-gel method for the prevention of biofilm formation on the surface of medical implanted devices. High-temperature processing of such coatings can lead to diffusion of nanosilver and reduce the amount of available silver particles for long-term effects. Using a low-temperature sol-gel method, we have successfully prepared silane-based matrices, phenyltriethoxysilane (PhTEOS), containing different amounts of Ag nanoparticles. The incorporation of a silver salt into the sol-gel matrix resulted in a desired silver release rate, i.e. high initial release rate followed by a lower sustained release for more than 15 days, as determined by inductively coupled plasma mass spectrometry (ICP-MS). Scanning electron microscopy (SEM) has been employed to investigate the morphology of the film surfaces before and after immersion in a nutrient-rich bacterial suspension of approximately 10⁸ CFU ml⁻¹, which was incubated for up to 30 days at 37 °C. It was found that thin films containing 35 nm particles could prevent the formation of biofilm for over 30 days. The presence of surface silver before and after 3, 9 and 15 days immersion was confirmed by x-ray photoelectron spectroscopy (XPS).

Biological responses of silver-coated thermosets: An in vitro and in vivo study

“…A similar silver release is described in the literature for most silver-based nanocomposite polymeric coatings [41][42][43]. Dissolution of silver in aqueous solutions is dependent on electrolyte type and concentration, pH, temperature and the presence of dissolved oxygen [44][45][46][47][48]; in particular, the presence of electrolytes in aqueous solution diminishes the electrostatic energy barrier to nanoparticle aggregation [45].…”

Immobilized silver nanoparticles enhance contact killing and show highest efficacy: elucidation of the mechanism of bactericidal action of silver

“…38 The high resolution Ag 3d spectrum could be deconvoluted including three additional peaks at binding energies of 367.7, 368.8, and 374.2 eV, thereby indicating the presence of small proportions of AgO, and Ag 2 O along with metallic silver. 39,40 To verify the presence of an oxide layer on the immobilized AgNPs, a high-resolution O 1s spectrum was further analysed. The deconvoluted O 1s spectrum appeared to be more complex.…”

Anti-quorum sensing activity of AgCl-TiO2 nanoparticles with potential use as active food packaging material

“…; Babapour et al . ; Naik and Kowshik ), and the anti‐QS activity of this traditionally used bioactive agent with potential in food preservation has not yet been explored. This study evaluates the anti‐QS activity of silver entrapped in a titanium dioxide (TiO 2 ) matrix.…”

“…There is a lot of literature about the antimicrobial activity of various forms of silver and silver composites and their mechanism of action (Panacek et al 2006;Kim et al 2007;Lara et al 2011;Brandt et al 2012;Naik et al 2013). However, only few studies have reported antibiofilm activity of silver and silver-based compounds (Stobie et al 2008;Kalishwaralal et al 2010;Babapour et al 2011;Naik and Kowshik 2014), and the anti-QS activity of this traditionally used bioactive agent with potential in food preservation has not yet been explored. This study evaluates the anti-QS activity of silver entrapped in a titanium dioxide (TiO 2 ) matrix.…”