Low-temperature solvent-based 3D printing of PLGA: a parametric printability study

Naseri, Emad; Butler, Haley; MacNevin, Wyatt; Ahmed, Marya; Ahmadi, Ali

doi:10.1080/03639045.2019.1711389

Cited by 31 publications

(18 citation statements)

References 38 publications

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“…Biomaterial inks were prepared by adding different concentrations of the mupirocin solution to PLGA (as shown in Table 1 ). PLGA concentration in the biomaterial ink was set at 80% ( w / v ) to maintain the optimum printable biomaterial ink concentration as described in our previous study [ 49 ]. The biomaterial inks were left for overnight magnetic stirring at 200 rpm at room temperature to yield homogeneous biomaterial inks.…”

Section: Methodsmentioning

confidence: 99%

“…Solvent removal: Proton nuclear magnetic resonance ( 1 H NMR) spectroscopy was used to confirm the complete removal of the solvent. Considering the vapor pressure of MEK at 20 °C (9.5 kPa), similar to our previous study [ 49 ], the printed scaffolds were dried in a vacuum flask connected to a vacuum line of a fume hood for one week. A scaffold was placed in 1 mL of deuterium oxide (D 2 O) 99.9% (Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA) to leach any remaining solvent into D 2 O overnight.…”

Section: Methodsmentioning

confidence: 99%

“…A scaffold was placed in 1 mL of deuterium oxide (D 2 O) 99.9% (Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA) to leach any remaining solvent into D 2 O overnight. The trace amount of MEK was detected by 1 H NMR spectroscopy (300 MHz, Bruker, MA, USA) with 16 scans setting [ 49 ].…”

Section: Methodsmentioning

confidence: 99%

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Development of 3D Printed Drug-Eluting Scaffolds for Preventing Piercing Infection

et al. 2020

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Herein, novel drug-eluting, bio-absorbable scaffold intended to cover piercing studs is introduced. This “biopierce” will stay in human tissue following piercing, and will slowly release an antimicrobial agent to prevent infection while the wound heals. Nearly 20% of all piercings lead to local infection. Therefore, it is imperative to develop alternative methods of piercing aftercare to prevent infection. Biopierces were made using mupirocin loaded poly-lactic-co-glycolic acid (PLGA) biomaterial ink, and a low-temperature 3D printing technique was used to fabricate the biopierces. Proton nuclear magnetic resonance (1H NMR) spectroscopy was used to confirm the complete removal of the solvent, and liquid chromatography high-resolution mass spectrometry (LC-HRMS) was used to confirm the structural integrity of mupirocin and to quantify the amount of the released drug over time. The efficacy of the biopierces against Staphylococcus aureus, one of the most common piercing-site pathogens, was confirmed over two weeks using in vitro antimicrobial susceptibility testing.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Development of 3D Printed Drug-Eluting Scaffolds for Preventing Piercing Infection

et al. 2020

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“…This polymer is approved by the FDA because its biocompatibility, and its physical strength make it widely used in various studies. [ 121–123 ] Naseri et al [ 124 ] used a 3D printing technique and methyl ethyl ketone (MEK) as a solvent to prepare PLGA constructs ( Figure 19 ).…”

Section: Hydrogel‐based Bioinksmentioning

confidence: 99%

Natural and Synthetic Bioinks for 3D Bioprinting

Khoeini

Nosrati

Akbarzadeh

et al. 2021

Advanced NanoBiomed Research

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Bioprinting offers tremendous potential in the fabrication of functional tissue constructs for replacement of damaged or diseased tissues. Among other fabrication methods used in tissue engineering, bioprinting provides accurate control over the construct's geometric and compositional attributes using an automated approach. Bioinks are composed of the hydrogel material and living cells that are critical process variables in the fabrication of functional, mechanically robust constructs. Appropriate cells can be encapsulated in bioinks to create functional tissue structures. Ideal bioinks are required to undergo a sol–gel transition consuming minimal processing time, and a plethora of chemical and physical crosslinking mechanisms are generally exploited to achieve high shape fidelity and construct stability. In contrast, crosslinking of hydrogel material at rapid rates can cause nozzle clogging, and hence, optimization of the bioink is often necessary. Bioinks can be formulated using natural or synthetic biomaterials, alone or in combination of these biomaterials. Herein, the various bioprinting methods are discussed; the natural, synthetic, or hybrid materials used as bioinks are analyzed; and the challenges, limitations, and future directions concerning the bioprinting technique are appraised.

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“…Other 3D printing applications have successfully addressed these issues by developing artifacts which stress a material using a variety of commonly used or difficult to create structures. [28][29][30][31][32][33][34] However, the materials available for 3D printing are not subject to the same constraints as those used in for bioprinting. For example, 3D printing materials do not require water (essential for cell functions) or low cytotoxicity (essential to avoid cell death).…”

Section: Introductionmentioning

confidence: 99%

Automated Image Analysis Methodologies to Compute Bioink Printability

et al. 2020

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The lack of suitable bioinks in bioprinting is a major limitation in tissue engineering and regenerative medicine. The reasons are multifaceted but can primarily be attributed to the contradictory requirements for bioinks to demonstrate desirable bioactivity while exhibiting high printability. Herein, methods are proposed and tools are provided to automatically quantitate the performance of bioinks using image analysis methods and differential geometry. Several artifact structures are used including a crosshatch to evaluate filament fusion, five-layer tube to evaluate stacked arc accuracy, overhang to test filament collapse, and a novel four-angled pattern to evaluate turn accuracy. Automatic measurements are 95.8% accurate in delineating pores of a crosshatch pattern, 96.5%, 86.0%, 80.3%, and 80.5% accuracy for each angle of a four-angled pattern, 98.9% and 97.9% accuracy for the external and internal radii of a fivelayer tube, and 90.6% and 99.0% accuracy for the height and width of a five-layer tube. This automation reduces the time and effort required to analyze a structure and also provides a standardized set of tools to compare different bioinks.

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Low-temperature solvent-based 3D printing of PLGA: a parametric printability study

Cited by 31 publications

References 38 publications

Development of 3D Printed Drug-Eluting Scaffolds for Preventing Piercing Infection

Development of 3D Printed Drug-Eluting Scaffolds for Preventing Piercing Infection

Natural and Synthetic Bioinks for 3D Bioprinting

Automated Image Analysis Methodologies to Compute Bioink Printability

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