Low Temperature Storage and in vitro Pollen Germination of Selected Spring Wheat Accessions

Baninasab, Bahram; Tabori, Mirko; Yu, Junjie; Zhang, Yuxue; Wang, Xuelian; Deschiffart, Ian; Khanizadeh, Shahrokh

doi:10.5539/jas.v9n9p1

Cited by 7 publications

(11 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, our study found that spring wheat genotypes maintained 6-14% germination at 4 °C after six days of storage. The data analysis emphasized the significant impact of genotypes on pollen viability and germination rates, as well as their interactions with storage temperatures and durations, corroborating earlier findings (Baninasab et al, 2017).…”

Section: Discussionsupporting

confidence: 85%

“…Our study highlighted that pollen viability and in vitro pollen germination were notably higher at 4 °C than at −20 °C and 22 °C across all selected spring wheat genotypes. In a prior study, spring wheat genotypes stored at 5 °C exhibited approximately 1.64% pollen germination after 24 h of storage but experienced a complete loss of viability, with 0.00% germination after 48 and 72 h (Baninasab et al, 2017). In contrast, our study found that spring wheat genotypes maintained 6-14% germination at 4 °C after six days of storage.…”

Section: Discussioncontrasting

confidence: 66%

“…Pollen viability plays a pivotal role in fertilization (Fernando et ). However, studies focusing on the short-term storage of spring wheat pollen at various temperatures are relatively scarce (Baninasab et al, 2017). Proper storage temperatures are crucial for preserving pollen viability (Alburquerque et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…However, achieving the right moisture level and thawing method is complex, making identification of the optimal preservation temperature essential (Sauve et al, 2000;Jia et al, 2022;Liu et al, 2023;Yuan et al, 2018). Limited data exist on wheat pollen storage at low temperatures, with one study reporting preservation at 5 ºC for one day (Baninasab et al, 2017). Evaluating pollen viability for numerous wheat lines is challenging due to the short lifespan post-shedding and variations in flowering periods.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Optimizing Wheat Pollen Preservation for Enhanced Viability and In Vitro Germination

Khan,

Naeem,

Shahzad

et al. 2023

Preprint

View full text Add to dashboard Cite

Wheat pollen, characterized by its short lifespan, exhibits rapid germination after anthesis. The preservation of wheat pollen is contingent upon environmental factors, including temperature, relative humidity, light, and wind. The objective is to develop a method for preserving wheat pollen effectively, specifically for the purpose of breeding. The short longevity of wheat pollen grains renders it impractical to conduct tests for pollen viability and in vitro germination on a large scale. Herein, the impact of storage temperatures and duration was assessed on pollen viability and in vitro germination to optimize storage conditions for preserving pollen viability. Pollen grains from 50 diverse spring wheat genotypes, each with three replicates, were harvested and stored at temperatures of 22°C, -20°C, and 4°C. Subsequently, pollen viability and in vitro germination rates were determined after storage of 1, 3, and 6 days. The results revealed that storage temperatures, durations, genotypes, and their interactions had a statistically significant impact on both pollen viability and in vitro germination. Notably, when pollen was kept at 22°C, almost all genotypes exhibited a loss of pollen viability and in vitro germination after 1, 3, and 6 days of storage. Likewise, storage at -20°C failed to extend pollen germination. However, at a storage temperature of 4°C, the pollen of 36 wheat genotypes exhibited a range of 6-14% in vitro pollen germination and remained viable even for 6 days. Simple linear regression analysis further revealed a significant negative correlation between pollen viability, in vitro germination, and storage time and temperature. In conclusion, our findings underscore 4°C as the optimal temperature for preserving pollen viability and in vitro pollen germination in spring wheat for up to 6 days. The results of the present study suggests that pollen viability of wheat is genotype de-pendent, storage temperature and storage duration. Thus, the 36 wheat genotypes identified during the present work could be efficiently maintained at 4◦C for short-term storage (6 days) and could be further used for genetics and breeding purposes.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussioncontrasting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Optimizing Wheat Pollen Preservation for Enhanced Viability and In Vitro Germination

Khan,

Naeem,

Shahzad

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Pollen preservation is crucial for breeding and genetic research [8]. Limited data exist on wheat pollen storage at low temperatures, with one study reporting preservation at 5 • C for one day [25]. Pollen life spans differ significantly between plant species, genotypes, and cultivars [26].…”

Section: Introductionmentioning

confidence: 99%

Optimizing Wheat Pollen Preservation for Enhanced Viability and In Vitro Germination

Khan,

Naeem,

Shahzad

et al. 2024

Agronomy

View full text Add to dashboard Cite

Wheat pollen, which is characterized by its short lifespan, exhibits rapid germination after anthesis. The preservation of wheat pollen is contingent upon environmental factors including temperature, relative humidity, light, and wind. The aim is to explicate the process for efficiently storing wheat pollen, particularly with regard to breeding. The short longevity of wheat pollen grains renders it impractical to conduct tests for pollen viability and in vitro germination on a large scale. Herein, the impact of storage temperatures and duration was assessed on pollen viability and in vitro germination in order to optimize storage conditions for preserving pollen viability. Pollen grains from 50 diverse spring wheat genotypes, each with three replicates, were harvested and stored at temperatures of 22 °C, −20 °C, and 4 °C. Subsequently, pollen viability and in vitro germination rates were determined after storage for 1, 3, and 6 days. The results revealed that storage temperatures, durations, genotypes, and their interactions had a statistically significant impact on both pollen viability and in vitro germination. Notably, when pollen was kept at 22 °C, almost all genotypes exhibited a loss of pollen viability and in vitro germination after 1, 3, and 6 days of storage. Likewise, storage at −20 °C failed to extend pollen germination. However, at a storage temperature of 4 °C, the pollen of 36 wheat genotypes exhibited a range of 6–14% for in vitro pollen germination and even remained viable for 6 days. The ANOVA revealed a substantial variation in grain number per spike between wheat genotypes, thereby highlighting the significant influence of genetic variations on grain yield. Moreover, a slight positive association between the viability of wheat pollen and the number of grains was found in the current study, suggesting that a variety of factors affect the number of grains produced. Simple linear regression analysis further revealed a significant negative correlation between pollen viability, in vitro germination, and storage time and temperature. In conclusion, our findings underscore that 4 °C is the optimal temperature for preserving pollen viability and in vitro pollen germination in spring wheat for up to 6 days. The results of the present study suggests that the pollen viability of wheat is dependent on genotype, storage temperature, and storage duration. Thus, the 36 wheat genotypes identified during the present work could be efficiently maintained at 4 °C for short-term storage (6 days) and could be further used for genetic and breeding purposes.

show abstract

Pollen viability, longevity, and function in angiosperms: key drivers and prospects for improvement

Althiab-Almasaud,

Teyssier,

Chervin

et al. 2023

Plant Reprod

View full text Add to dashboard Cite

Low Temperature Storage and in vitro Pollen Germination of Selected Spring Wheat Accessions

Cited by 7 publications

References 14 publications

Optimizing Wheat Pollen Preservation for Enhanced Viability and In Vitro Germination

Optimizing Wheat Pollen Preservation for Enhanced Viability and In Vitro Germination

Optimizing Wheat Pollen Preservation for Enhanced Viability and In Vitro Germination

Pollen viability, longevity, and function in angiosperms: key drivers and prospects for improvement

Contact Info

Product

Resources

About