Lower Levels of Expression of FATA2 Gene Promote Longer Siliques with Modified Seed Oil Content in Arabidopsis thaliana

Wang, Qian; Huang, Wenda; Jiang, Qi; Lian, Jinpan; Sun, Jun; Xu, Hong; Zhao, Huixian; Liu, Zhenlan

doi:10.1007/s11105-013-0612-1

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…In Arabidopsis, the FatA and FatB acyl-ACP thioesterases are plastid-targeted soluble enzymes (Joyard et al, 2010;Wang et al, 2013). To determine the subcellular localization of FatM during AMS, we fused GFP to the C-terminal end of FatM and expressed the gene fusion from the native FatM promoter.…”

Section: Researchmentioning

confidence: 99%

Arbuscular mycorrhiza‐specific enzymes FatM and RAM2 fine‐tune lipid biosynthesis to promote development of arbuscular mycorrhiza

et al. 2017

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Summary During arbuscular mycorrhizal symbiosis (AMS), considerable amounts of lipids are generated, modified and moved within the cell to accommodate the fungus in the root, and it has also been suggested that lipids are delivered to the fungus. To determine the mechanisms by which root cells redirect lipid biosynthesis during AMS we analyzed the roles of two lipid biosynthetic enzymes (FatM and RAM2) and an ABC transporter (STR) that are required for symbiosis and conserved uniquely in plants that engage in AMS. Complementation analyses indicated that the biochemical function of FatM overlaps with that of other Fat thioesterases, in particular FatB. The essential role of FatM in AMS was a consequence of timing and magnitude of its expression. Lipid profiles of fatm and ram2 suggested that FatM increases the outflow of 16:0 fatty acids from the plastid, for subsequent use by RAM2 to produce 16:0 β‐monoacylglycerol. Thus, during AMS, high‐level, specific expression of key lipid biosynthetic enzymes located in the plastid and the endoplasmic reticulum enables the root cell to fine‐tune lipid biosynthesis to increase the production of β‐monoacylglycerols. We propose a model in which β‐monoacylglycerols, or a derivative thereof, are exported out of the root cell across the periarbuscular membrane for ultimate use by the fungus.

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Section: Researchmentioning

confidence: 99%

Arbuscular mycorrhiza‐specific enzymes FatM and RAM2 fine‐tune lipid biosynthesis to promote development of arbuscular mycorrhiza

et al. 2017

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“…The T‐DNA insertion cals7 mutants showed growth and reproduction defects, including short root, stem and silique as well as reduced male fertility and seed number per silique (Xie et al , ) The Arabidopsis FATA2 gene encodes Acyl‐ACP thioesterase involved in fatty acid biosynthetic process, which was expressed d in roots, seedlings, leaves, stems, flowers, with especially high abundance in siliques. The fata2 T‐DNA insertion mutants produced longer siliques with more but smaller seeds as well as increased oil content (Wang et al , ). Lysophosphatidyl acyltransferase (LPAT) is a key enzyme for adjusting the metabolic conversion of lysophosphatidic acid into exclusive phosphatidic acids in numerous tissues (Kim et al , ).…”

Section: Other Proteinsmentioning

confidence: 99%

Genetic and signalling pathways of dry fruit size: targets for genome editing‐based crop improvement

Hussain

Shi

Scheben

et al. 2020

Plant Biotechnology Journal

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Summary Fruit is seed‐bearing structures specific to angiosperm that form from the gynoecium after flowering. Fruit size is an important fitness character for plant evolution and an agronomical trait for crop domestication/improvement. Despite the functional and economic importance of fruit size, the underlying genes and mechanisms are poorly understood, especially for dry fruit types. Improving our understanding of the genomic basis for fruit size opens the potential to apply gene‐editing technology such as CRISPR/Cas to modulate fruit size in a range of species. This review examines the genes involved in the regulation of fruit size and identifies their genetic/signalling pathways, including the phytohormones, transcription and elongation factors, ubiquitin‐proteasome and microRNA pathways, G‐protein and receptor kinases signalling, arabinogalactan and RNA‐binding proteins. Interestingly, different plant taxa have conserved functions for various fruit size regulators, suggesting that common genome edits across species may have similar outcomes. Many fruit size regulators identified to date are pleiotropic and affect other organs such as seeds, flowers and leaves, indicating a coordinated regulation. The relationships between fruit size and fruit number/seed number per fruit/seed size, as well as future research questions, are also discussed.

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“…The highest thioesterase activity was found in crude extracts of oil palm pulp [33]. Wang [34] cloned FATB from the seed kernel of LpFATB by the homologous cloning method, and found that the contents of eicosenoic acid (20:1) and oleic acid (18:1) in the seeds of LPFATB transformed strains with Col-0 as the backgrounds were decreased to a certain extent, while the contents of palmitic acid (16:0) were significantly increased. In this study, the FATB enzyme gene was highly expressed in the flesh of Seedless and Tenera oil palm species and was positively correlated with the contents of free stearic acid and palmitic acid, while negatively correlated with the contents of free myristate acid.…”

Section: Discussionmentioning

confidence: 99%

Metabonomics and Transcriptomic Analysis of Free Fatty Acid Synthesis in Seedless and Tenera Oil Palm

Wei,

Yang,

John Martin

et al. 2024

IJMS

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Oil palm, a tropical woody oil crop, is widely used in food, cosmetics, and pharmaceuticals due to its high production efficiency and economic value. Palm oil is rich in free fatty acids, polyphenols, vitamin E, and other nutrients, which are beneficial for human health when consumed appropriately. Therefore, investigating the dynamic changes in free fatty acid content at different stages of development and hypothesizing the influence of regulatory genes on free fatty acid metabolism is crucial for improving palm oil quality and accelerating industry growth. LC-MS/MS is used to analyze the composition and content of free fatty acids in the flesh after 95 days (MS1 and MT1), 125 days (MS2 and MT2), and 185 days (MS3 and MT3) of Seedless (MS) and Tenera (MT) oil palm species fruit pollination. RNA-Seq was used to analyze the expression of genes regulating free fatty acid synthesis and accumulation, with differences in genes and metabolites mapped to the KEGG pathway map using the KEGG (Kyoto encyclopedia of genes and genomes) enrichment analysis method. A metabolomics study identified 17 types of saturated and 13 types of unsaturated free fatty acids during the development of MS and MT. Transcriptomic research revealed that 10,804 significantly different expression genes were acquired in the set differential gene threshold between MS and MT. The results showed that FabB was positively correlated with the contents of three main free fatty acids (stearic acid, myristate acid, and palmitic acid) and negatively correlated with the contents of free palmitic acid in the flesh of MS and MT. ACSL and FATB were positively correlated with the contents of three main free fatty acids and negatively correlated with free myristate acid. The study reveals that the expression of key enzyme genes, FabB and FabF, may improve the synthesis of free myristate in oil palm flesh, while FabF, ACSL, and FATB genes may facilitate the production of free palmitoleic acid. These genes may also promote the synthesis of free stearic acid and palmitoleic acid in oil palm flesh. However, the FabB gene may inhibit stearic acid synthesis, while ACSL and FATB genes may hinder myristate acid production. This study provides a theoretical basis for improving palm oil quality.

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Lower Levels of Expression of FATA2 Gene Promote Longer Siliques with Modified Seed Oil Content in Arabidopsis thaliana

Cited by 7 publications

References 34 publications

Arbuscular mycorrhiza‐specific enzymes FatM and RAM2 fine‐tune lipid biosynthesis to promote development of arbuscular mycorrhiza

Arbuscular mycorrhiza‐specific enzymes FatM and RAM2 fine‐tune lipid biosynthesis to promote development of arbuscular mycorrhiza

Genetic and signalling pathways of dry fruit size: targets for genome editing‐based crop improvement

Metabonomics and Transcriptomic Analysis of Free Fatty Acid Synthesis in Seedless and Tenera Oil Palm

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