Lower quality bovine embryos may be successfully used for sex determination

Lopatářová, M.; Čech, Svatopluk; Krontorád, Petr; Holý, L; Hlavicova, J.; Doležel, R.

doi:10.17221/1884-vetmed

Cited by 4 publications

(6 citation statements)

References 25 publications

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“…In IVP embryos biopsied by aspiration, the mortality rate was 10.3% and the pregnancy rate was 31.7% after ET of fresh embryos (Alonso et al, 2009). In this study, the gender was determined in 91.3% of embryos for fresh transfer and in 87.5% of embryos intended for freezing (Table 1), which accords with our previous work with whole, intact (88.7%; Lopatarova et al, 2007) and splitted embryos (89.4-92%; Lopatarova et al, 2008). Our results are similar to those published by other authors (85-95%) using embryos from superovulated donors (Thibier and Nibart, 1995;Shea, 1999;Li et al, 2007;Yu et al, 2007) as well as IVP embryos (Lopes et al, 2001;Hasler et al, 2002;Tominaga and Hamada, 2004).…”

Section: Discussionsupporting

confidence: 88%

“…In our previous experiments (Lopatarova et al, 2007(Lopatarova et al, , 2008 for isolation of embryonic cells we used the blade biopsy for fresh embryo transfer. In this study, we have introduced the aspiration procedure to the biopsy of embryonic cells which can retain embryos with high developmental competence and minimize the damage to the zona pellucida before the freeze-thaw procedure.…”

Section: Discussionmentioning

confidence: 99%

“…From the embryos specified for fresh transfer, a small portion of the embryo was cut off by transzonal incision using a microsurgical blade as described (Lopatarova et al, 2007). No holding pipette was used during cutting, only PBS without proteins was employed for fixation of embryos.…”

Section: Embryo Biopsymentioning

confidence: 99%

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Conception rate after sex determination and cryopreservation of D7 bovine embryos:

Lopatářová¹,

Čech²,

Krontorád³

et al. 2010

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to evaluate conception rate after sex determination and subsequent freezing of Day 7 (D 7 ) bovine embryos and to compare it with transfer of fresh female embryos. High quality embryos obtained from superovulated donors were biopsied and the embryonic cells (5-10) were analysed by PCR using specific primers for the Y-chromosome determinant. Fresh embryos were blade biopsied (n = 172) and subsequently transferred (ET) ipsilaterally to synchronized recipients. Selected embryos (n = 112) were biopsied by cell aspiration and cryopreserved for later transfer. Sex determination was successfully completed in 91.3% of fresh group embryos (44% female) and in 87.5% of freeze-thawed group embryos (45.9% female). The achieved pregnancy rate was 56.5% after fresh transfer of sexed (female) embryos whereas intact embryos (control 1) implanted in 61.9% recipients. 95.6% (43/45) of embryos survived biopsy and cryopreservation. After transfer of freeze-thawed sexed (female) embryos, pregnancy was established in 48.8% of recipients. A similar conception rate was found in intact embryos (50.7%, control 2). The results clearly demonstrate that the microsurgical technique used and subsequent PCR sex analysis allow the rapid commercial exchange of genetic resources on the basis of fresh or frozen sex-desired embryos in embryo transfer programs.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

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Conception rate after sex determination and cryopreservation of D7 bovine embryos:

Lopatářová¹,

Čech²,

Krontorád³

et al. 2010

Vet. Med. - Czech

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“…On the other hand, only 3 to 6 cells are used as a sufficient number (Taneja et al, 1998;Park et al, 2001;Wu et al, 2004) for sex determination. In our previous experiments (Lopatarova et al, 2007) we isolated six to 12 cells from high-quality compacted morulae or blastocysts and the sex status was determined in nearly 90% of embryos. Because this number of cells seems to be sufficient for the safe sex analysis, we used a similar cell number obtained from one half of demi-embryos in this study.…”

Section: Discussionmentioning

confidence: 99%

Sex determination in bisected bovine embryos and conception rate after the transfer of female demi-embryos

Lopatářová¹,

Čech²,

Krontorád³

et al. 2008

Vet. Med. - Czech

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The aim of this study was to evaluate the efficiency of sex determination after microsurgical splitting of D7 (Day 7) bovine embryos and to test the conception rate after subsequent transfer of female demi-embryos. High-quality morulae (n = 100) and early blastocysts (n = 123) obtained from superovulated donors were microsurgically bisected and blastomeres biopsied from one half of bisected embryos were analysed by PCR using specific primers for the Y-chromosome determinant. The female demi-embryos were transferred (ET) in pairs (bilateral) or individually (ipsilateral) to synchronized recipients. Sex determination was successfully completed in 92% of morulae (42.4% female) and 89.4% of early blastocysts (43.6% female). Conception rates were 56.5% (30.4% identical twins) and 48.8% (19.5% identical twins) after bilateral (n = 46) and ipsilateral (n = 82) transfers, respectively. The number of foetuses in relation to the number of original embryos before splitting was 40/46 (87%) and 40/41 (97.6%) after bilateral and ipsilateral transfers of demi-embryos, respectively. The results document that the microsurgical bisection in combination with PCR sex analysis represents a rapid and reliable approach to increase an amount of sex-desired calves in embryo transfer programs.

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“…Microsurgical intervention was carried out with the Lica micromanipulator (Leica, Germany). From the embryos specified for fresh transfer, a small portion (5-10%) of each embryo was cut off by ranszonal incision using a microsurgical blade as described by (Lopatarova et al, 2007).…”

Section: Embryo Biopsymentioning

confidence: 99%

Effect of Seminal Ph Value at Insemination on Sex Ratio of Bovine Embryos

Shamiah¹,

Hussein²,

El-Dein³

et al. 2011

Journal of Animal and Poultry Production

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The objective of this study was to evaluate the effect of changes in seminal pH value at insemination on sex ratio of bovine embryos and confirm embryo sexing using Polymerase Chain Reaction (PCR). Eighteen Friesian cows superovulated with 2500 IU of PMSG and divided into three groups were used in this study. Cows in the first (G1), second (G2) and third (G3) group were artificially inseminated by diluted semen with pH values of 6.9 (control), 5.4 (acidic) and 8.4 (alkaline), respectively. Results showed insignificant (P≥0.05) effect of seminal pH value on ovulatory response and quality of recovered embryos. Change of seminal pH value to acidosis in (G2) produced higher (P<0.01) number of females (19/32, 59.4%) than males (13/32, 40.6%). Change of seminal pH value to alkaline (G3) produced higher (P<0.01) number of males (61.8%) than females (38.2%). However, sex ratio of cows inseminated with control semen (pH=6.9, G1) was 51.9% males: 48.2% females. In conclusion, change of seminal pH value to alkaline tended to produce more males, while acidic semen tended to produce more females. Based on these results motility of spermatozoa bearing Y-chromosome may be affected by the acidic condition of the semen, while X-chromosome had an opposite trend.

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Lower quality bovine embryos may be successfully used for sex determination

Cited by 4 publications

References 25 publications

Conception rate after sex determination and cryopreservation of D7 bovine embryos:

Conception rate after sex determination and cryopreservation of D7 bovine embryos:

Sex determination in bisected bovine embryos and conception rate after the transfer of female demi-embryos

Effect of Seminal Ph Value at Insemination on Sex Ratio of Bovine Embryos

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