Loxosceles gaucho venom gland proteome: A new perspective on Loxosceles venom biochemical composition

Valladão, Rodrigo; Coelho, Guilherme Rabelo; Silva, Daiane Laise da; Beraldo-Neto, Emidio; Pimenta, Daniel C.; Chiariello, Thiago Mathias; Auada, Aline Vivian Vatti; Wen, Fan Hui; Lopes, Adriana Rios

doi:10.1016/j.toxicon.2019.12.046

Cited by 2 publications

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“…Moreover, studies have shown similarities between components in the spider’s digestive system and its venom, including cysteine-rich molecules. Among these, NcTI, identified as a time-dependent inhibitor, has been detected in the digestive juice of the spider Nephilingis cruentata , while similar molecules have been observed in other spider species such as Acanthoscurria geniculata , Stegodyphus mimosarum , and Loxosceles gaucho [ 87 , 88 , 89 , 90 , 91 ]. These findings further underscore the intriguing strategies spiders employ in their survival tactics.…”

Section: Venom Peptides’ Roles In Spider Survivalmentioning

confidence: 88%

Spider-Venom Peptides: Structure, Bioactivity, Strategy, and Research Applications

Guo,

Wang

et al. 2023

Molecules

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Spiders (Araneae), having thrived for over 300 million years, exhibit remarkable diversity, with 47,000 described species and an estimated 150,000 species in existence. Evolving with intricate venom, spiders are nature’s skilled predators. While only a small fraction of spiders pose a threat to humans, their venoms contain complex compounds, holding promise as drug leads. Spider venoms primarily serve to immobilize prey, achieved through neurotoxins targeting ion channels. Peptides constitute a major part of these venoms, displaying diverse pharmacological activities, and making them appealing for drug development. Moreover, spider-venom peptides have emerged as valuable tools for exploring human disease mechanisms. This review focuses on the roles of spider-venom peptides in spider survival strategies and their dual significance as pharmaceutical research tools. By integrating recent discoveries, it provides a comprehensive overview of these peptides, their targets, bioactivities, and their relevance in spider survival and medical research.

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Section: Venom Peptides’ Roles In Spider Survivalmentioning

confidence: 88%

Spider-Venom Peptides: Structure, Bioactivity, Strategy, and Research Applications

Guo,

Wang

et al. 2023

Molecules

View full text Add to dashboard Cite

show abstract

“…Although there is a lot of data on venom proteomic analysis, the evaluation of the similarities between the spiders´ MMD/digestive fluid and venom protein content was only possible recently due to the use of high-throughput techniques to perform transcriptomic and proteomic analysis of the spiders Nephilingis cruentata (digestive fluid and MMD) 1 ; Acanthoscurria geniculata (digestive fluid and abdomen) 2 ; Stegodyphus mimosarum (digestive fluid) 2 ; Loxosceles gaucho (MMD) 1 , 12 . These studies revealed the presence of several molecules similar to venom toxins and peptidase inhibitors expressed and translated to protein in the digestive systems of spiders.…”

Section: Introductionmentioning

confidence: 99%

Spiders’ digestive system as a source of trypsin inhibitors: functional activity of a member of atracotoxin structural family

Neto

Valladão

Coelho

et al. 2023

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Spiders are important predators of insects and their venoms play an essential role in prey capture. Spider venoms have several potential applications as pharmaceutical compounds and insecticides. However, transcriptomic and proteomic analyses of the digestive system (DS) of spiders show that DS is also a rich source of new peptidase inhibitor molecules. Biochemical, transcriptomic and proteomic data of crude DS extracts show the presence of molecules with peptidase inhibitor potential in the spider Nephilingis cruentata. Therefore, the aims of this work were to isolate and characterize molecules with trypsin inhibitory activity. The DS of fasting adult females was homogenized under acidic conditions and subjected to heat treatment. After that, samples were submitted to ion exchange batch and high-performance reverse-phase chromatography. The fractions with trypsin inhibitory activity were confirmed by mass spectrometry, identifying six molecules with inhibitory potential. The inhibitor NcTI (Nephilingis cruentata trypsin inhibitor) was kinetically characterized, showing a KD value of 30.25 nM ± 8.13. Analysis of the tertiary structure by molecular modeling using Alpha-Fold2 indicates that the inhibitor NcTI structurally belongs to the MIT1-like atracotoxin family. This is the first time that a serine peptidase inhibitory function is attributed to this structural family and the inhibitor reactive site residue is identified. Sequence analysis indicates that these molecules may be present in the DS of other spiders and could be associated to the inactivation of prey trypsin (serine peptidase) ingested by the spiders.

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Loxosceles gaucho venom gland proteome: A new perspective on Loxosceles venom biochemical composition

Cited by 2 publications

References 0 publications

Spider-Venom Peptides: Structure, Bioactivity, Strategy, and Research Applications

Spider-Venom Peptides: Structure, Bioactivity, Strategy, and Research Applications

Spiders’ digestive system as a source of trypsin inhibitors: functional activity of a member of atracotoxin structural family

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