This paper presents some results of research on the diversity of the Theaceae in Pu Hoat Nature Reserve, Nghe An province, from May 2019 to February 2022. Total 41 species belonging to 9 genera of Theaceae family were collected and identified. There were 1 genera and 13 species found as new records for the plant list of Pu Hoat Naturre reserve, published in 2019. Plant resources of Theaceae comprised 21 species representing 51.22% of total species and falling into 6 group of uses, such as: medicinal plants, timber plants, ornamental plants, tea drink plants, oil plants, poison plants. Medicinal plants are the largest group with 14 species represent 66.67% of total of plant resources and 34.15% of total species. The Spectrum of Biology (SB) of the Theaceae in Pu Hoat is summarized, as follows: 26.83%Me + 70.73 %Mi + 2.44%Na. The family of Theaceae in Pu Hoat Nature Reserve is mainly comprised of the endemic and subendemic element (70.73%), Asian tropical element (21.95%), Northerm temperate elements (7.32%).