LPCAT1 acts as an independent prognostic biomarker correlated with immune infiltration in hepatocellular carcinoma

Li, Lan; Wang, Xiao; Ding, Yanni; Hui, Nini; Su, Bingjie; Yang, Min

doi:10.1186/s40001-022-00854-1

Cited by 10 publications

(4 citation statements)

References 35 publications

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“…As one of the lysophosphatidylcholine acyltransferase (LPCAT) family, the LPCAT1 protein is an enzyme essential for phosphatidylcholine metabolism and regulation of phosphatidylcholine composition [ 30 ]. LPCAT1 is also used in the prognosis of multiple tumors, such as breast cancer, colorectal cancer, and hepatocellular carcinoma [ 31 – 33 ]. Little is known about LPCAT1 in allergic diseases.…”

Section: Discussionmentioning

confidence: 99%

Identification and analysis of lipid metabolism-related genes in allergic rhinitis

et al. 2023

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Background Studies have shown that the lipid metabolism mediator leukotriene and prostaglandins are associated with the pathogenesis of allergic rhinitis (AR). The aim of this study was to identify key lipid metabolism-related genes (LMRGs) related to the diagnosis and treatment of AR. Materials and methods AR-related expression datasets (GSE75011, GSE46171) were downloaded through the Gene Expression Omnibus (GEO) database. First, weighted gene co-expression network analysis (WGCNA) was used to get AR-related genes (ARRGs). Next, between control and AR groups in GSE75011, differentially expressed genes (DEGs) were screened, and DEGs were intersected with LMRGs to obtain lipid metabolism-related differentially expressed genes (LMR DEGs). Protein-protein interaction (PPI) networks were constructed for these LMR DEGs. Hub genes were then identified through stress, radiality, closeness and edge percolated component (EPC) analysis and intersected with the ARRGs to obtain candidate genes. Biomarkers with diagnostic value were screened via receiver operating characteristic (ROC) curves. Differential immune cells screened between control and AR groups were then assessed for correlation with the diagnostic genes, and clinical correlation analysis and enrichment analysis were performed. Finally, real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was made on blood samples from control and AR patients to validate these identified diagnostic genes. Results 73 LMR DEGs were obtained, which were involved in biological processes such as metabolism of lipids and lipid biosynthetic processes. 66 ARRGs and 22 hub genes were intersected to obtain four candidate genes. Three diagnostic genes (LPCAT1, SGPP1, SMARCD3) with diagnostic value were screened according to the AUC > 0.7, with markedly variant between control and AR groups. In addition, two immune cells, regulatory T cells (Treg) and T follicular helper cells (TFH), were marked variations between control and AR groups, and SMARCD3 was significantly associated with TFH. Moreover, SMARCD3 was relevant to immune-related pathways, and correlated significantly with clinical characteristics (age and sex). Finally, RT-qPCR results indicated that changes in the expression of LPCAT1 and SMARCD3 between control and AR groups were consistent with the GSE75011 and GSE46171. Conclusion LPCAT1, SGPP1 and SMARCD3 might be used as biomarkers for AR.

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Section: Discussionmentioning

confidence: 99%

Identification and analysis of lipid metabolism-related genes in allergic rhinitis

et al. 2023

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“…As one of the lysophosphatidylcholine acyltransferase (LPCAT) family, the LPCAT1 protein is an enzyme essential for phosphatidylcholine metabolism and regulation of phosphatidylcholine composition [17]. LPCAT1 is also used in the prognosis of multiple tumors, such as breast cancer, colorectal cancer, and hepatocellular carcinoma [18][19][20]. Little is known about LPCAT1 in allergic diseases.…”

Section: Discussionmentioning

confidence: 99%

Identification and analysis of lipid metabolism-related genes in allergic rhinitis

Tao

Zhu

Wang

et al. 2023

Preprint

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Background:Studies have shown that the lipid metabolism mediator leukotriene is associated with the pathogenesis of allergic rhinitis (AR). The aim of this study was to identify key lipid metabolism-related genes (LMRGs) related to the diagnosis and treatment of AR. Material and methods: AR-related expression datasets (GSE75011, GSE46171) were downloaded through the Gene Expression Omnibus (GEO) database. First, weighted gene coexpression network analysis (WGCNA) was used to get AR-related genes (ARRGs). Next, between control and AR guoups in GSE75011, differentially expressed genes (DEGs) were screened, and DEGs were intersected with LMRGs to obtain lipid metabolism-related differentially expressed genes (LMR DEGs). Protein‒protein interaction (PPI) networks were constructed for these LMR DEGs. Hub genes were then identified through stress, radiality, closeness and edge percolated component (EPC) analysis and intersected with the ARRGs to obtain candidate genes. Biomarkers with diagnostic value were screened via receiver operating characteristic (ROC) curves.Differential immune cells screened between control and AR groups were then assessed for correlation with the diagnostic genes, and clinical correlation analysis and enrichment analysis were performed. Finally, reverse transcription-polymerase chain reaction(RT‒qPCR) was made on blood samples from control and AR patients to validate these identified diagnostic genes. Results: 73 LMR DEGs were obtained, which were involved in biological processes such as metabolism of lipids and lipid biosynthetic processes. Sixty-six ARRGs and 22 hub genes were intersected to obtain four candidate genes. Three diagnostic genes (LPCAT1, SGPP1, SMARCD3) with diagnostic value were screened according to the AUC > 0.7, with markedly variant between control and AR groups. In addition, two immune cells, regulatory T cells (TReg) and T follicular helper cells (TFH), were marked variations between control and AR groups, and SMARCD3 was significantly associated with TFH. Moreover, SMARCD3 was relevant to immue-related pathways, and correlated significantly with clinical characteristics (age and sex). Finally, RT‒qPCR results indicated that changes in the expression of LPCAT1 and SMARCD3 between control and AR groups were consistent with the GSE75011 and GSE46171. Conclusion: LPCAT1, SGPP1 and SMARCD3 might be used as biomarkers for AR.

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“…For instance, LPCAT3 was correlated with immune infiltration in acute myeloid leukemia [ 7 ]. LPCAT1 acts as an independent prognostic biomarker correlated with immune infiltration in hepatocellular carcinoma [ 19 ]. Our study further constructed LPCATs score and predicated that high LPCATs score was associated with immune-activated TME.…”

Section: Discussionmentioning

confidence: 99%

Pan-cancer analysis identifies LPCATs family as a prognostic biomarker and validation of LPCAT4/WNT/β-catenin/c-JUN/ACSL3 in hepatocellular carcinoma

Liang

et al. 2023

Aging

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Lipid remodeling regulators are now being investigated as potential therapeutic targets for cancer therapy as a result of their involvement, which includes promoting cancer cells’ adaptation to the restricted environment. Lysophosphatidylcholine acyltransferases (LPCATs, LPCAT1-4) are enzymes that regulate the remodeling of bio-membranes. The functions of these enzymes in cancer are largely unknown. In the current study, we found that genes belonging to the LPCAT family participated in tumor advancement and were strongly linked to dismal prognosis in many different malignancies. We constructed the LPCATs scores model and explored this model in pan-cancer. Malignant pathways in pan-cancer were positively related to LPCATs scores, and all pathways had strong links to the tumor microenvironment (TME). Multiple immune-associated features of the TME in pan-cancer were likewise associated with higher LPCATs scores. In addition, the LPCATs score functioned as a prognostic marker for immune checkpoint inhibitor (ICI) therapies in patients with cancer. LPCAT4 enhanced cell growth and cholesterol biosynthesis by up-regulating ACSL3 in hepatocellular carcinoma (HCC). WNT/β-catenin/c-JUN signaling pathway mediated LPCAT4’s regulation on ACSL3. These findings demonstrated that genes in the LPCAT family might be used as cancer immunotherapy and prognosis-related biomarkers. Specifically, LPCAT4 could be a treatment target of HCC.

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LPCAT1 acts as an independent prognostic biomarker correlated with immune infiltration in hepatocellular carcinoma

Cited by 10 publications

References 35 publications

Identification and analysis of lipid metabolism-related genes in allergic rhinitis

Identification and analysis of lipid metabolism-related genes in allergic rhinitis

Identification and analysis of lipid metabolism-related genes in allergic rhinitis

Pan-cancer analysis identifies LPCATs family as a prognostic biomarker and validation of LPCAT4/WNT/β-catenin/c-JUN/ACSL3 in hepatocellular carcinoma

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