LPGAT1 controls the stearate/palmitate ratio of phosphatidylethanolamine and phosphatidylcholine in sn-1 specific remodeling

Abstract

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Identification and characterization of LPLAT7 as an sn-1-specific lysophospholipid acyltransferase

“…As mentioned above, LPLAT7 was shown to have a role in determining the stearate-to-palmitate ratio in PL species ( 22 ). Our lipidomic analyses showed that loss of LPLAT7 increased not only C16:0-containing PLs but also C18:1-containing PLs ( Fig.…”

“…They also demonstrated that LPLAT7/LPGAT1 preferred sn -2-acyl LPE over sn -1-acyl LPE using a recombinant enzyme expressed in E. coli . They claimed that LPLAT7/LPGAT1 had no LPLAT activity against LPC ( 22 ). The result is incompatible with our present results, which we discuss below.…”

Acyltransferases in the first step of glycerophospholipid synthesis have redundant and non-redundant roles insn-1 acyl chain regulation, ether lipid levels, and cell survival

“…For example, PS having oleic acid (18:1) at the sn- 2 position interacts with cholesterol when its sn- 1 acyl chain is stearic acid (18:0), but not palmitic acid (16:0) 22 . The enzyme LPGAT1 was shown to remodel GPLs, especially PE, to increase 18:0 levels at the sn- 1 position, and its deficiency is partially lethal 23,24 . The existence of LPGAT1 as a regulator of 18:0 in PE might also explain why GPAT4 mutants had no changes in this acyl chain for PE, in contrast to PC, PI, and PS.…”

An organelle-specific photoactivation and dual-isotope labeling strategy reveals phosphatidylethanolamine metabolic flux

“…It has been recently shown that lysophosphatidylglycerol acyltransferase 1 (LPGAT1) is a key remodeling enzyme located in the ER and involved in the regulation of the stearate/palmitate ratio at the sn1 position of LysoPE species. 37 It is possible that LPGAT1 is responsible for the 15 N-PE16:0-13 C18:1 (i.e. 15 N 13 C-PE34:1) remodeling into 15 N-PE18:0-13 C18:1.…”