Lrp as a potential transcriptional regulator involved in stress response in Haloferax mediterranei

Matarredona, Laura; Camacho, Mónica; Bautista, Vanesa; Bonete, María-José; Esclapez, Julia

doi:10.1016/j.biochi.2023.01.012

Cited by 4 publications

(2 citation statements)

References 41 publications

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“…In Hfx. mediterranei Lrp seems to be involved in a general response against stress factors [ 50 ] and mediates regulation in the stress response, especially under N-limiting conditions and in the presence of cobalt [ 51 ]. On the other hand, the MarR family regulators include a series of transcription factors that modulate genes in response to environmental signals by acting as sensors in changing environments.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Bioinformatics Analysis of the Biodiversity of Lsm Proteins in the Archaea Domain

et al. 2023

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The Sm protein superfamily includes Sm, like-Sm (Lsm), and Hfq proteins. Sm and Lsm proteins are found in the Eukarya and Archaea domains, respectively, while Hfq proteins exist in the Bacteria domain. Even though Sm and Hfq proteins have been extensively studied, archaeal Lsm proteins still require further exploration. In this work, different bioinformatics tools are used to understand the diversity and distribution of 168 Lsm proteins in 109 archaeal species to increase the global understanding of these proteins. All 109 archaeal species analyzed encode one to three Lsm proteins in their genome. Lsm proteins can be classified into two groups based on molecular weight. Regarding the gene environment of lsm genes, many of these genes are located adjacent to transcriptional regulators of the Lrp/AsnC and MarR families, RNA-binding proteins, and ribosomal protein L37e. Notably, only proteins from species of the class Halobacteria conserved the internal and external residues of the RNA-binding site identified in Pyrococcus abyssi, despite belonging to different taxonomic orders. In most species, the Lsm genes show associations with 11 genes: rpl7ae, rpl37e, fusA, flpA, purF, rrp4, rrp41, hel308, rpoD, rpoH, and rpoN. We propose that most archaeal Lsm proteins are related to the RNA metabolism, and the larger Lsm proteins could perform different functions and/or act through other mechanisms of action.

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Section: Resultsmentioning

confidence: 99%

Comprehensive Bioinformatics Analysis of the Biodiversity of Lsm Proteins in the Archaea Domain

et al. 2023

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“…The Lrp/AsnC family of transcriptional factors has been described as one of the most common archaeal global and local transcription regulators that mediate survival in harsh conditions, including metal-induced stress [ 41 , 49 , 50 ]. In Hfx.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide transcriptional response to silver stress in extremely halophilic archaeon Haloferax alexandrinus DSM 27206 T

Buda,

Szekeres,

Tudoran

et al. 2023

BMC Microbiol

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Background The extremely halophilic archaeon Haloferax (Hfx.) alexandrinus DSM 27206 T was previously documented for the ability to biosynthesize silver nanoparticles while mechanisms underlying its silver tolerance were overlooked. In the current study, we aimed to assess the transcriptional response of this haloarchaeon to varying concentrations of silver, seeking a comprehensive understanding of the molecular determinants underpinning its heavy metal tolerance. Results The growth curves confirmed the capacity of Hfx. alexandrinus to surmount silver stress, while the SEM–EDS analysis illustrated the presence of silver nanoparticles in cultures exposed to 0.5 mM silver nitrate. The RNA-Seq based transcriptomic analysis of Hfx. alexandrinus cells exposed to 0.1, 0.25, and 0.5 mM silver nitrate revealed the differential expression of multiple sets of genes potentially employed in heavy-metal stress response, genes mostly related to metal transporters, basic metabolism, oxidative stress response and cellular motility. The RT-qPCR analysis of selected transcripts was conducted to verify and validate the generated RNA-Seq data. Conclusions Our results indicated that copA, encoding the copper ATPase, is essential for the survival of Hfx. alexandrinus cells in silver-containing saline media. The silver-exposed cultures underwent several metabolic adjustments that enabled the activation of enzymes involved in the oxidative stress response and impairment of the cellular movement capacity. To our knowledge, this study represents the first comprehensive analysis of gene expression in halophillic archaea facing increased levels of heavy metals.

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Global Lrp regulator protein from Haloferax mediterranei: Transcriptional analysis and structural characterization

Matarredona,

García-Bonete,

Guío

et al. 2024

International Journal of Biological Macromolecules

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Lrp as a potential transcriptional regulator involved in stress response in Haloferax mediterranei

Cited by 4 publications

References 41 publications

Comprehensive Bioinformatics Analysis of the Biodiversity of Lsm Proteins in the Archaea Domain

Comprehensive Bioinformatics Analysis of the Biodiversity of Lsm Proteins in the Archaea Domain

Genome-wide transcriptional response to silver stress in extremely halophilic archaeon Haloferax alexandrinus DSM 27206 T

Global Lrp regulator protein from Haloferax mediterranei: Transcriptional analysis and structural characterization

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