LsaYAB7, A Homologous Gene of FILAMENTOUS FLOWER, Participating In The Regulation of Adaxial-Abaxial Polarity of Leaves In Lettuce

Abstract

Background: The plant-specific YABBY transcription factor plays important roles in the development of lateral organs, the establishment of adaxial-abaxial polarity and abiotic stress response. However, the function of YABBY gene family in lettuce (Lactuca sativa), an important leaf vegetable is still unclear yet. Results: In this study, we analyzed the chromosomal distribution, gene structure and cis-transcriptional elements of the YABBY gene family in lettuce. Phylogenetic analysis of YABBY genes in lettuce, Arabidopsis, maize and rice classified them into five groups. In addition, the expression profile of YABBY genes in lettuce indicated that they may play distinct functions in different tissues and developmental processes. The subcellular localization analysis and transactivation assay showed that LsaYAB7 probably functions as a transcription factor in the nucleus. Furthermore, the ectopic expression of LsaYAB7 exhibited abaxially curled leaves, not only in Arabidopsis, but also in lettuce. Conclusion: Altogether, LsaYAB7 is a key functional gene in determining the adaxial-abaxial polarity of lettuce leaves. Our study laid foundation for the molecular research of functional genes in lettuce.

Genome-Wide Identification, and In-Silico Expression Analysis of YABBY Gene Family in Response to Biotic and Abiotic Stresses in Potato (Solanum tuberosum)

“…Until now, YABBY genes have been identified and characterized in different plant species, and the number of genes varied from 6 to 23. The number of YABBY genes reported are 9 in Ananas comosus [ 6 ], 7 in Vitis vinifera [ 12 ], 11 in Cucurbita moschata , 12 in Cucurbita maxima , 11 in Cucurbita pepo [ 15 ], 16 in Phyllostachys edulis [ 2 ], 11 in Brassica rapa , 11 in Brassica oleracea , 12 in Brassica nigra , 19 in Brassica juncea , 21 in Brassica napus [ 16 , 17 ], 17 in Glycine max [ 11 ], 12 in Gossypium arboreum , 12 in Gossypium raimondii , 23 in Gossypium hirsutum [ 3 ], 21 in Triticum aestivum [ 18 ], 8 in Phaseolus vulgaris (Common bean) [ 19 ], 9 in Solanum lycopersicum [ 20 ], 8 in Oryza sativa [ 21 , 22 ], 13 in Zea mays [ 23 ], and 9 in Lactuca sativa [ 24 ].…”