LSD1 Regulates Pluripotency of Embryonic Stem/Carcinoma Cells through Histone Deacetylase 1-Mediated Deacetylation of Histone H4 at Lysine 16

Yin, Feng; Lan, Rongfeng; Zhang, Xiaoming; Zhu, Linyu; Chen, Fangfang; Xu, Zhengshuang; Liu, Yuqing; Ye, Tao; Sun, Hong; Lu, Fei; Zhang, Hui

doi:10.1128/mcb.00631-13

Cited by 64 publications

(88 citation statements)

References 50 publications

(65 reference statements)

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“…However, the G1 cell cycle rate of human ES cells treated with AA alone was 45.82%, which did not have the significant arrest as that of TMSN-AA. Therefore, these results indicated that AA delivered by TMSNs can effectively and significantly induce the differentiation of human ES cells by blocking the cell cycle in the G1 phase [38,39].…”

Section: Aa Delivered By Msns Cause G1 Cell Cycle Arrest In Human Es mentioning

confidence: 78%

Ascorbic acid delivered by mesoporous silica nanoparticles induces the differentiation of human embryonic stem cells into cardiomyocytes

Ren

Han

et al. 2015

Materials Science and Engineering: C

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Section: Aa Delivered By Msns Cause G1 Cell Cycle Arrest In Human Es mentioning

confidence: 78%

Ascorbic acid delivered by mesoporous silica nanoparticles induces the differentiation of human embryonic stem cells into cardiomyocytes

Ren

Han

et al. 2015

Materials Science and Engineering: C

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“…However, the high expression of SOX2 and OCT4 was frequently found in many cancers and showed a significant association with poor prognosis and disease-free survival. 13,[18][19][20][21] Understanding the mechanism of how stemness factors are regulated is of great significance for cancer pathogenesis and clinical therapy. To uncover the relationship between cell cycle oscillation and stemness maintenance such as OCT4 and SOX2 expression, we arrested PA-1 cells with nocodazole (50 ng/mL) and detected SOX2 using specific antibody.…”

Section: Sox2 Is Highly Modified In M Phasementioning

confidence: 99%

“…AURKA wildly presents rich expression in kinds of tumors and is considered as target for inhibitors development and cancer therapy. 17 In this study, we used human ovarian cancer PA-1 cells, a rapid proliferating cell lines with rich SOX2 and OCT4 expression, 18 to study the regulatory connection of cell cycle and SOX2. Exactly, we discovered that SOX2 is heavily modified in mitosis.…”

Section: Introductionmentioning

confidence: 99%

Mitotic phosphorylation of SOX2 mediated by Aurora kinase A is critical for the stem-cell like cell maintenance in PA-1 cells

Wang

et al. 2016

Cell Cycle

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Transcription factor SOX2 is multiple phosphorylated. However, the kinase and the timing regulating SOX2 phosphorylation remains poorly understood. Here we reported mitotic phosphorylation of SOX2 by Aurora kinase A (AURKA). AURKA inhibitors (VX680, Aurora kinase Inhibitor I) but not PLK1 inhibitors (BI2536, CBB2001) eliminate the mitotic phosphorylation of SOX2. Consistently, siRNA inhibition of AURKA can eliminate mitotic SOX2 phosphorylation. Ser220 and Ser251 are two sites that identified for mitotic phosphorylation on SOX2. Moreover, SOX2 mutants (S220A and S251A) can promote SOX2 induced OCT4 re-expression in differentiated cells. These findings reveal a novel regulation mechanism of SOX2 phosphorylation mediated by AURKA in mitosis and its function in stem cell pluripotency maintenance in cancer cells.

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“…The mRNA levels of the target genes were quantified by real-time PCR using SYBR green (Takara) in an ABI Prism 7300 real-time PCR system (Applied Biosystems). The housekeeping gene β-actin as the control gene has been utilized to normalize RT-PCR analysis (Yin et al 2014). Sequences of primers are in Table 1.…”

Section: Rna Isolation and Quantitative Rt-pcrmentioning

confidence: 99%

“…The re-expression assay was adapted from Yin et al (2014). In brief, wild-type pCMV10-3Flag-PEDF, pCMV10-3Flag-Hes1 and pCMV10-3Flag-Hes5 plasmids were constructed by fusing the full-length cDNAs of human PEDF, Hes1 and Hes5 with the Flag tag in the pCMV10-3Flag vector (Clontech).…”

Section: Re-expression and Sirna Rescuementioning

confidence: 99%

The roles of nanocarriers on pigment epithelium-derived factor in the differentiation of human cardiac stem cells

et al. 2015

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Over the past decade, adult stem cells have attracted great attention because of their ability to potentially regenerate desired tissues or entire organs. With the emergence of nanomaterial-based gene therapy, adult stem cells have been considered as a proper tool for the biomedical field. In this study, we utilized organically modified silica (ORMOSIL) nanoparticles to deliver small interfering RNA (siRNA) against pigment epithelium-derived factor (PEDF) and induce the differentiation of human cardiac stem cells (CSCs). We found that the down-regulation of PEDF can inhibit the proliferation of human CSCs and induce cell differentiation. To further study the mechanism, we have tested the Notch signalling pathway genes, Hes1 and Hes5, and found that their expressions were inhibited by the PEDF down-regulation. Furthermore, with the restoration of PEDF, both the proliferation of human CSCs and expressions of Hes1 and Hes5 were recovered. Our results suggest for the first time the use of ORMOSIL as nanocarriers for the delivery of PEDF siRNA in human CSCs, and demonstrated the cooperation between PEDF and the Notch signalling pathway in maintaining the self-renewal and pluripotency of stem cells. PEDF as the essential controller in differentiation may be a promising target for the regulation of cardiac homeostasis and damage repair, which opens new treatment strategies using nanomaterials for heart disease therapy.

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LSD1 Regulates Pluripotency of Embryonic Stem/Carcinoma Cells through Histone Deacetylase 1-Mediated Deacetylation of Histone H4 at Lysine 16

Cited by 64 publications

References 50 publications

Ascorbic acid delivered by mesoporous silica nanoparticles induces the differentiation of human embryonic stem cells into cardiomyocytes

Ascorbic acid delivered by mesoporous silica nanoparticles induces the differentiation of human embryonic stem cells into cardiomyocytes

Mitotic phosphorylation of SOX2 mediated by Aurora kinase A is critical for the stem-cell like cell maintenance in PA-1 cells

The roles of nanocarriers on pigment epithelium-derived factor in the differentiation of human cardiac stem cells

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