&lt;b&gt;Armazenamento criogênico de sementes de pinhão manso (Jatropha curcas L.) Euphorbiaceae&lt;/b&gt;&lt;br&gt;doi: 10.5007/2175-7925.2010v23n1p27

Goldfarb, M.; Duarte, M. de L. R.; Mata, M. E. R. M. C.

doi:10.5007/2175-7925.2010v23n1p27

Cited by 10 publications

(7 citation statements)

References 6 publications

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“…), which facilities the formation of intracellular ice crystals that cause ruptures in the endomembrane system and compromise cellular compartmentalization (Goldfarb et al . ; Wesley‐Smith et al . ).…”

Section: Discussionmentioning

confidence: 99%

Seed storage of Brazilian cacti species in different threat categories

Santos

Hassemer

Meiado

2018

Plant Species Biology

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Cactaceae is considered the fifth most endangered taxonomic group. In light of this, the aim of this study was to evaluate the efficiency of different low‐temperature storage techniques in maintaining the viability of seeds of cacti in different threat categories. Seeds of six cacti taxa were stored in a cold chamber (8°C), a freezer (−5°C), in liquid nitrogen (−196°C) and at room temperature (25–27°C) for a period of 0, 1, 3, 6, 9 and 13 months. At each evaluation interval we removed a seed sample for each taxon studied, which was distributed into four repetitions of 25 seeds maintained at room temperature under 12‐h light/dark photoperiods. We evaluated the germinability, mean germination time and synchronization index. Most of the studied taxa presented germinability of above 50%, which was influenced by time and by storage temperatures. Also, most taxa stored at room temperature presented a significant reduction in germinability, whereas almost all taxa showed maintenance of the seed viability when stored in a cold chamber, a freezer or liquid nitrogen. This response can be justified by the reduction of the seed metabolism and the degradation of the reserve compounds of the seeds while at lower temperatures. Our results indicate that storage at low temperatures is an effective method for the conservation of cacti seeds and can be used for the formation of artificial seed banks of threatened cacti species.

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Section: Discussionmentioning

confidence: 99%

Seed storage of Brazilian cacti species in different threat categories

Santos

Hassemer

Meiado

2018

Plant Species Biology

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“…Accordingly, Astronium urundeuva (urunday) seeds maintained their viability when cryopreserved with 6% m c water content (Medeiros et al, 1992). Similarly, Jatropha curcas L. (Barbados nut) seeds maintained their vigour index when stored in liquid nitrogen or in vapour (Goldfarb et al, 2010) and their high viability when cryopreserved with 8% m c water content (Silva et al, 2011). Tabebuia chrysotricha (golden trumpet tree) seeds showed higher germination when cryopreserved with 4% m c water content (Tresena et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation as an alternative for conservation of Anacardium humile achene (Monkey nut)

Barbosa¹,

Sales²,

Silva³

et al. 2019

Aust J Crop Sci

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The present study aimed to evaluate the possibility of using cryopreservation method for conservation of cajuzinho-do-cerrado achene (Anacardium humile). To accomplish this aim, the physiological quality of cryopreserved achenes was compared with the quality of samples stored under other conditions. The quality of cryopreserved achenes with different water contents was also evaluated to establish a water-content limit for seed-viability maintenance. Achenes with physiological maturity were stored in liquid nitrogen at -196 °C with the following water contents: 28 (initial content) 14, 12, 8 and 5% moisture content (m c ). Subsequently, the achenes were subjected to 3 thawing methods: slow/gradual, fast and microwave thawing. Achenes were also subjected to different six conservation methods: cryopreservation in liquid nitrogen (LN) at -196 °C, cryopreservation using 10% cryoprotectants (Dimethyl sulfoxide (DMSO) or Glycerol), 10 °C biochemical oxygen demand (BOD) incubator, -80 °C ultra-low freezer and room temperature (25 °C ±3), with testing after storage in these environments for 5 and 10 months. The treatment effect on achene, physiological quality was assessed by seed germination (percentage) and vigour (germination speed index, electrical conductivity and root and shoot length). The results showed that reduction in the water content considerably reduced the germinating potential of Anacardium humile achenes for cryopreservation. Conversely, the thawing methods had no effect on the physiological quality of cryopreserved achenes; thereby, allowing the choice of the most practical thawing method (microwave). We also concluded that cryopreservation in liquid nitrogen may be used as a viable alternative for conservation of Anacardium humile achene.

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“…According to these authors, in a low-temperature germplasm bank, not only the cryopreservation process but also the thawing method must be considered, since the faster the thawing of seeds, the better is the preservation of their physiological characteristics. This procedure may prevent the ice crystals within the cell, which would lead to destruction of the membrane and consequently cause cell death (Goldfarb et al, 2010). Kholina & Voronkova (2008) using 103 species and Voronkova & Kholina (2010) using 11 species endemic to eastern Russia found that cryostored seeds subjected to slow thawing for 2 h, under laboratory environmental conditions (20−25 °C), had no loss or physiological damage.…”

Section: Dimethyl Sulfoxide Use In Cryopreservationmentioning

confidence: 99%

“…The ex situ preservation shows some advantages compared to in situ, such as reduced need for space, lower expenses and labor costs, easier germplasm exchange, and reduced genetic erosion (Engelmann, 1991). Seed preservation is the easiest, most common and efficient means of ex situ preservation, since the seeds ensure genetic variability, are easily collected, and occupy small spaces (Li & Pritchard, 2009;Goldfarb et al, 2010;Pence, 2010).…”

Section: Introductionmentioning

confidence: 99%

Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen

et al. 2017

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The objective of this study was to evaluate the effects of two cryoprotectants followed by thawing on the physiological potential of Genipa americana L. seeds. Two experiments testing 12 treatments were conducted, one for each cryoprotectant, both in a factorial scheme of 6 × 2 (cryoprotectant concentrations × thawing methods). We tested 0, 5, 10, 15, 20, and 25% for dimethyl sulfoxide, and 0, 0.25, 0.5, 0.75, 1.0, and 1.25 M for sucrose. The thawing methods were fast (38 °C for 30 min) and slow (25 °C for 4 h). The seeds were immersed in the cryoprotectant solution for 3 h, stored for 120 h, and then thawed. The seeds were then sown in substrate (sand and vermiculite, 1:1). Emergence percentage along with speed index, length, fresh and dry matter mass of seedlings were evaluated. Dimethyl sulfoxide and sucrose can be used as cryoprotectants in G. americana seeds. Thawing should be slow when treating seeds with dimethyl sulfoxide.

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<b>Armazenamento criogênico de sementes de pinhão manso (Jatropha curcas L.) Euphorbiaceae</b><br>doi: 10.5007/2175-7925.2010v23n1p27

Cited by 10 publications

References 6 publications

Seed storage of Brazilian cacti species in different threat categories

Seed storage of Brazilian cacti species in different threat categories

Cryopreservation as an alternative for conservation of Anacardium humile achene (Monkey nut)

Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen

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