9Species interaction plays a vital role in structuring communities by stimulating behavioral 10 responses in temporal niche affecting the sympatric associations and prey-predator 11 relationships. We studied relative abundance indices (RAI) and activity patterns of each 12 species, temporal overlap between sympatric species, and effects of moon cycle on predator-13 prey relationships, through camera-trapping in tropical semi-evergreen forests of Manas 14 National Park. A total of 35 species were photo-captured with 16214 independent records over 15 7337 trap nights. Overall, relatively high number of photographs was obtained for large 16 herbivores (11 species, n=13669), and low number of photographs were recorded for large 17 carnivores (five species, n=657). Activity periods were classified into four categories: diurnal 18 (day-time), nocturnal (night-time), crepuscular (twilight), and cathemeral (day and night time) 19 of which 52% records were found in diurnal period followed by 37% in nocturnal phase 20 whereas only 11% photographs during twilight. Small carnivores were strictly nocturnal 21 (leopard cat and civets) or diurnal (yellow-throated marten and mongooses); whereas large 22 carnivores were cathemeral (tiger, leopard, clouded leopard and Asiatic black bear). Analysis 2 23 of activity patterns throughout the 24-h cycle revealed a high degree of temporal overlap 24 (>60%) among most of the sympatric species; however, differences in the activity peaks were 25 found between most of the species pairs. Moon phase was classified according to the 26 percentage of visible moon surface as new (0-25%), waxing (25-50%), waning (50-75%) and 27 full moon (75-100%). Moon phase did not have any correlation with activity of large carnivore 28 and large prey. The large carnivore followed the feed and starve pattern of cyclic activity. The 29 activity of small carnivore was influenced negatively by moonlight ( partial correlation r = -0.221, 30 p<0.01). The result suggests that large carnivores were active non-differentially across moon 31 phases; however, small carnivores showed significantly high activity in darker nights. These 32 patterns indicate that small predators may differ their activity temporally as an anti-predator 33 strategy or otherwise to increase their foraging efficiency. 34 Keywords 35 Camera-trapping, tropical forest, temporal overlap, sympatric, moon phase, prey-predator 36 relationship 37 42 carnivores, interspecific interactions are particularly relevant because of their role in the top-43 down control and also serve as flagship species in the conservation of biodiversity in many 44 terrestrial ecosystems [4]. Though, given the vital role of consumers and through trophic 45 cascades, changes in the environment could promote an increase of medium-sized carnivores 3 46 or mesopredators, due to top predator removal [5] which can cause substantial changes in the 47 dynamics of interaction among sympatric species [6], with adverse effects on subordinate 48 species. Thus, to minimize risks, subo...