&lt;b&gt;INVOLVEMENT OF STEROID HORMONES IN &lt;/b&gt;&lt;b&gt;GONADOTROPIN-INDUCED TESTICULAR MATURATION IN MALE JAPANESE EEL &lt;i&gt;(ANGUILLA JAPONICA&lt;/i&gt;) &lt;/b&gt;

Miura, Takeshi; Yamauchi, Kazuyo; Takahashi, Hiroya; Nagahama, Yoshitaka

doi:10.2220/biomedres.12.241

Cited by 83 publications

(47 citation statements)

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“…While until now 17,20b-P has been shown to be involved in sperm function in teleosts (Baynes and Scott, 1985;Miura et al, 1991bMiura et al, , 1992, for those species in which this hormone is lacking a similar role may be taken by 17,20b,21-P (Thomas et al, 1997) or 17,20a-P (Vermeirssen et al, 2000). However, in Lusitanian toadfish 17,20b,21-P stays elevated for a long period and follows the pattern of swimbladder hypertrophy.…”

Section: Discussionmentioning

confidence: 99%

Morphometric changes and sex steroid levels during the annual reproductive cycle of the Lusitanian toadfish, Halobatrachus didactylus

Modesto

Canário

2003

General and Comparative Endocrinology

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Section: Discussionmentioning

confidence: 99%

Morphometric changes and sex steroid levels during the annual reproductive cycle of the Lusitanian toadfish, Halobatrachus didactylus

Modesto

Canário

2003

General and Comparative Endocrinology

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“…In some teleosts such as japanese eel, Anguilla japonica Temminck et Schlegel, 1846, rainbow trout and Chum salmon, Oncorhynchus keta (Walbaum, 1792), it is suggested that 17α,20β-dihydroxy-4-pregnen-3-one (DHP) is related to the regulation of sperm maturation in SF (Miura et al 1991a, b;1992). In this regard, the DHP indirectly mediate the increasing of seminal plasma pH, which in turn increases the sperm content of cAMP, thereby allowing the acquisition of potential of motility (Miura et al 1991a(Miura et al , 1992(Miura et al , 1995. In Persian sturgeon and stellate (Bayunova et al 2006), the BS levels of DHP did not show significant change both in mature and non-mature males.…”

Section: Steroids In Seminal Fluidmentioning

confidence: 99%

Comparative analysis of milt quality and steroid levels in blood and seminal fluid of Persian sturgeon males, Acipenser persicus during final maturation induced by hormonal treatment

2010

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Steroids both in seminal fluid (SF) and blood serum (BS) as well as the milt quality (sperm motility and sperm production) were investigated during final maturation of Persian sturgeon. The BS levels of testosterone (T), 11-Ketotestosterone (11-KT), progesterone (P), 17α,20β,21-trihydroxy-4-pregnen-3-one (20βS), cortisol (C) and 17α,hydroxyprogesterone (OHP) elevated after pituitary preparation (PP) treatment and then decreased during stripping period for spermiating males. Such elevations did not occur for non-spermiating individuals and steroids remained in basal levels after PP treatment until the end of stripping period. For both groups (spermiating and non-spermiating fish), the BS levels of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) did not show significant changes during experiment. During stripping period, the values of all tested steroids were significantly lower in SF than in BS of spermiating males. SF levels of 20βS and 11-KT showed a decreasing trend and the other steroids were unchanged during this period. Significant positive correlations were found between the values of 20βs and 11-KT in BS with their levels in SF. Also, BS and SF levels of 20βs and 11-KT were positively correlated with sperm motility characteristics (percentage and duration of motility) and sperm production (sperm density and milt volume), respectively. The results showed the probable involvement of 20βs, P, OHP, T, 11-KT and C in final maturation of Persian sturgeon, especially 20βs and 11-KT had good correlations with qualitative parameters of milt. The lower levels of steroids in SF than those in BS might also be essential for viability of Persian sturgeon spermatozoa. Probably, there are mechanisms that stabilize the concentrations of a number of hormones in the SF.

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“…Progestins regulate oocyte maturation (8) by binding to an oocyte plasma membrane receptor, inhibiting oocyte adenylate cyclase, followed by reduced cAMP-dependent protein kinase activity, which induces the activation of maturation promoting factor via Cdc25, eventually triggering the resumption of division I of meiosis (7,8). In fish spermatogenesis, progestin also plays an important role in spermiation and sperm maturation (9)(10)(11). A major progestin in teleost fish, 17␣,20␤-dihydroxy-4-pregnen-3-one (DHP), induces sperm hydration (9) and acquisition of sperm motility in some species (10,11).…”

mentioning

confidence: 99%

“…In fish spermatogenesis, progestin also plays an important role in spermiation and sperm maturation (9)(10)(11). A major progestin in teleost fish, 17␣,20␤-dihydroxy-4-pregnen-3-one (DHP), induces sperm hydration (9) and acquisition of sperm motility in some species (10,11). A related progestin, 17␣, 20␣-dihydroxy-4-pregnen-3-one, is the spermiation-inducing hormone in amphibia (12).…”

mentioning

confidence: 99%

Progestin is an essential factor for the initiation of the meiosis in spermatogenetic cells of the eel

Miura

Higuchi

Ozaki

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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132

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Meiosis is an indispensable process of sexual reproduction. However, detailed information on the regulatory mechanisms that initiate meiosis is not available. Progestins are important steroids regulating final maturation in male and female vertebrates. In male teleosts, it is known that progestin induces spermiation and sperm maturation. However, a role for progestin in early spermatogenesis or meiosis has not yet been described. In this study, we examined the functions of progestin on the initiation of meiosis in male Japanese eel. A natural progestin in teleost fish 17␣,20␤-dihydroxy-4-pregnen-3-one (DHP) and its receptors were present in the testis at an early stage of spermatogenesis. By using an eel testicular culture system, DHP was shown to induce DNA replication in spermatogonia. Although 11-ketotestosterone, a known initiator of spermatogenesis, also stimulated DNA synthesis in spermatogonia, antibodies against DHP prevented DNA replication when added during the period in which meiosis was initiated. DHP treatment also induced the expression of meiosis-specific markers, such as DmcI and Spo11. Furthermore, Spo11 expression and synaptonemal complexes, specific features of the meiotic prophase, were detected in testicular fragments cultured with DHP in some germ cells that showed morphological characteristics of undifferentiated spermatogonia. We conclude that DHP, a progestin, is an essential factor for the initiation of meiosis.in vitro culture ͉ Japanese eel ͉ spermatogenesis ͉ androgen ͉ Spo11

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<b>INVOLVEMENT OF STEROID HORMONES IN </b><b>GONADOTROPIN-INDUCED TESTICULAR MATURATION IN MALE JAPANESE EEL <i>(ANGUILLA JAPONICA</i>) </b>

Cited by 83 publications

References 0 publications

Morphometric changes and sex steroid levels during the annual reproductive cycle of the Lusitanian toadfish, Halobatrachus didactylus

Morphometric changes and sex steroid levels during the annual reproductive cycle of the Lusitanian toadfish, Halobatrachus didactylus

Comparative analysis of milt quality and steroid levels in blood and seminal fluid of Persian sturgeon males, Acipenser persicus during final maturation induced by hormonal treatment

Progestin is an essential factor for the initiation of the meiosis in spermatogenetic cells of the eel

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