1997
DOI: 10.2220/biomedres.18.389
|View full text |Cite
|
Sign up to set email alerts
|

<b>lmmunoreactive proinsulin detected by enzyme-linked immunosorbent </b><b>assay </b>

Abstract: A sensitive enzyme-linked immunosorbent assay for human proinsulin was developed by the modification of the method reported using monoclonal antibodies. In the present method, two monoclonal antibodies, an anti-C-peptide antibody bound to microtiter plate, and a biotin-labeled anti-insulin antibody were uesd. This assay was specific for proinsulin and failed to detect both insulin and C-peptide. The minimal detection limit of this assay was approximately 0.1 pmol/1. lmmunoreactive proinsulin levels in serum of… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2

Citation Types

0
2
0

Year Published

2000
2000
2002
2002

Publication Types

Select...
2

Relationship

0
2

Authors

Journals

citations
Cited by 2 publications
(2 citation statements)
references
References 0 publications
0
2
0
Order By: Relevance
“…The insulin assay had < 0.005% cross‐reactivity with human proinsulin and none with C‐peptide [18]. Plasma proinsulin was determined by an ELISA [19] in which cross‐reaction with insulin and C‐peptide was 0.00015% and 0.00004%, respectively, at 1 nmol/l, and none at the lower concentrations.…”
Section: Methodsmentioning
confidence: 99%
“…The insulin assay had < 0.005% cross‐reactivity with human proinsulin and none with C‐peptide [18]. Plasma proinsulin was determined by an ELISA [19] in which cross‐reaction with insulin and C‐peptide was 0.00015% and 0.00004%, respectively, at 1 nmol/l, and none at the lower concentrations.…”
Section: Methodsmentioning
confidence: 99%
“…Plasma proinsulin was measured by a two-site sandwich enzyme-linked immunosorbent assay (Yuka Medias, Co., Tokyo, Japan) with biotinylated anti-human insulin monoclonal antibodies (HUI-018, DAKO A/S, Denmark) and anti-human C-peptide monoclonal antibody (PEP-001, DAKO A/S, Denmark). The detailed characterization of this method was as previously described [17]. Both intact proinsulin and proinsulin conversion intermediates (split and des-proinsulin) were fully recognized in this proinsulin assay.…”
Section: Methodsmentioning
confidence: 99%