&lt;b&gt;&lt;i&gt;Galla chinensis &lt;/i&gt;&lt;/b&gt;Compounds Remineralize Enamel Caries Lesions in a Rat Model

Zhang, Tieting; Guo, Hao; Liu, Xuejun; Chu, Jinpu; Zhou, Xuedong

doi:10.1159/000445036

Cited by 26 publications

(24 citation statements)

References 38 publications

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“…Figure 7 shows TEM and SAED analyses of mineral phases formed during spontaneous mineralization experiments in the absence and presence of peptide (50 μg/mL). Mineral phase identification was based on the broad diffuse SAED ring pattern ( Figure 7A , 15 min, insets) and the morphology of TEM observations ( Figure 7A , 15 min), which exhibited spherical shapes that are consistent with the known morphology of ACP ( Eanes, 2001 ; Zhang et al, 2016 ), similarly reported in other studies ( Mahamid et al, 2008 ). Then, a rapid decrease in pH was observed from 7.2 to 6.5, in correspondence to the formation of plate-like particles.…”

Section: Resultssupporting

confidence: 81%

“…After the remineralization treatment, the mineral density and profiles of each specimen were determined with an Inveon micro-CT system (Siemens AG, Germany). The micro-CT X-ray source was set at an operating voltage of 80 kV and 500 μA, providing a 10-μm isotropic voxel resolution ( Zhang et al, 2016 ). A mineral density calibration curve was generated by scanning three hydroxyapatite disks (50, 250, and 750 mg/mL) as reference phantoms ( Songsiripradubboon et al, 2014 ).…”

Section: Methodsmentioning

confidence: 99%

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Remineralization Efficacy of an Amelogenin-Based Synthetic Peptide on Carious Lesions

et al. 2018

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Objective: The aim of this study was to evaluate the remineralization efficacy of an amelogenin-based peptide on initial enamel carious lesions in vitro. Furthermore, we attempted to provide insights into the possible mechanism of the remineralization, including determining the calcium-binding properties of the peptide and its effects on calcium phosphate mineralization.Methods: The peptide comprising the N-terminus and the C-terminus of porcine amelogenin was synthesized by Synpeptide Co., Ltd. Fifty specimens were randomly assigned to five immersing treatment groups for 12 days: remineralizing medium only; 12.5 μg/mL peptide + remineralizing medium; 25 μg/mL peptide + remineralizing medium; 50 μg/mL peptide + remineralizing medium; fluoride + remineralizing medium. After immersion, mean mineral loss before and after remineralization of each specimen was determined using micro-CT. Mean mineral gain after remineralization was calculated. Calcium binding properties were measured by Isothermal titration calorimetry (ITC). TEM and Fourier transform-infrared were used to determine the effects of the peptide on calcium phosphate mineralization.Results: A significant decrease in mineral loss after remineralization process in all groups was observed (p < 0.05). Treatment in remineralizing medium resulted in the lowest mineral gain while the fluoridated treatment exhibited the highest mineral gain among all groups. Inclusion of synthetic peptide in the remineralizing medium exhibited a higher mineral gain and the gain of 50 μg/mL group was greater than that of the 25 μg/mL group. No significant difference in mineral gain was observed between the remineralizing medium only group and the 12.5 μg/mL peptide group (p > 0.05). ITC values showed that the Ca2+-binding affinity of the peptide is about 9.914 × 104M−1. Furthermore, the peptide was found to inhibit calcium phosphate precipitation and stabilize amorphous calcium phosphate formation for more than 2 h and finally transform into ordered hydroxyapatite crystals.Conclusion: Specific concentrations of the amelogenin-based synthetic peptide promoted in vitro remineralization, with higher concentrations exhibiting significantly greater remineralization. This study presented evidence suggesting that the peptide may act as a Ca2+carrier as well as a regulating factor. When the stabilizing calcium and phosphorus ions bind with the peptide they become biologically available for the remineralization of deeper carious lesions, while also regulated by the peptide to transform into ordered hydroxyapatite crystals.

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Section: Resultssupporting

confidence: 81%

Section: Methodsmentioning

confidence: 99%

Remineralization Efficacy of an Amelogenin-Based Synthetic Peptide on Carious Lesions

et al. 2018

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“…These results could explain the GCE formed of different polyphenol compounds, which might slow down the remineralization of dentin surface by time, and offer more channels for ion transferring through the surface layer to the lesion body. 39…”

Section: Discussionmentioning

confidence: 99%

Effect of Galla Chinensis on Remineralization of Early Dentin Lesion

2020

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Objective This study aimed to investigate the efficacy of the Galla chinensis extract (GCE) on early dentin lesion remineralization. Materials and Methods Seventy-two dentin specimens were divided into three groups according to the treatment solution. In group S1, specimens were treated with GCE; in group S2, the specimens were treated with sodium fluoride (NaF); meanwhile, specimens of group S3 were treated with distilled water (DW). Each group was further subdivided into two subgroups according to the treatment time (1 minute and 5 minutes). An in vitro pH-cycling model for 12 days was done. Subsequently, surface microhardness (SMH) of the specimens, elemental analysis, and their micromorphological appearance were evaluated. Statistical Analysis Data were statistically analyzed. One-way analysis of variance was used to compare numerical (parametric) data between more than two separate groups followed by post hoc Tukey. Results There was no significant difference between the mean SMH of dentin between NaF and GCE groups. Regarding the time, the 5-minute treatment with NaF and DW groups recorded higher mean SMH value of dentin than the 1-minute treatment group. Meanwhile, for GCE groups, the 1-minute immersion recorded higher mean SMH value than the 5-minute immersion without any significant difference between them. The microhardness results were confirmed by environmental scanning electron microscope and energy dispersive X-ray analysis results. Conclusion GCE could be used as an effective alternative for dentin remineralization.

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“…121 Ayrıca flor ve nanohidroksi apatit içeren remineralizasyon ajanları ile birlikte kullanımı değerlendirilmiş ve sinerjistik etkiye sahip olduğu gösterilmiştir. 122 Klinik kullanıma uyarlanabilmesi için toksikoloji testlerini de içeren ileri çalışmalara ihtiyaç vardır.…”

Section: Galla Chinensisunclassified

Current Overview of Remineralization Materials and Technologies

Demir¹

2020

Yeditepe J Dent

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<b><i>Galla chinensis </i></b>Compounds Remineralize Enamel Caries Lesions in a Rat Model

Cited by 26 publications

References 38 publications

Remineralization Efficacy of an Amelogenin-Based Synthetic Peptide on Carious Lesions

Remineralization Efficacy of an Amelogenin-Based Synthetic Peptide on Carious Lesions

Effect of Galla Chinensis on Remineralization of Early Dentin Lesion

Current Overview of Remineralization Materials and Technologies

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