&lt;b&gt;Ovicidal effect of the essential oils from 18 Brazilian &lt;i&gt;Piper&lt;/i&gt; species: controlling &lt;i&gt;Anticarsia gemmatalis&lt;/i&gt; (Lepidoptera, Erebidae) at the initial stage of development

Krinski, Diones; Foerster, Luís A.; Deschamps, Cícero

doi:10.4025/actasciagron.v40i1.35273

Cited by 34 publications

(26 citation statements)

References 65 publications

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“…A mortalidade observada nos ácaros rajado pode ocorrer devido à constituição complexa dos óleos essenciais, capazes de reduzir o número de ovos e a inibição da oviposição alterando a reprodução dos insetos (Krinski et al, 2018). Estudos anteriores relatam a capacidade dos óleos essenciais em reduzir a fecundidade em T. urticae (Pontes et al, 2010;Ribeiro et al, 2016), mas sem indicação se esta redução na fecundidade se devia às propriedades dos óleos ou á morte de ácaros.…”

Section: Discussionunclassified

Caracterização fitoquímica e avaliação do potencial acaricida e inseticida do óleo essencial de Hymeneae courbaril L. var. courbaril sobre o ácaro-rajado e o gorgulho do milho

Mercês¹,

Silva-Bessa²,

Malafaia

et al. 2018

J. Env. Anal. Progr.

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As propriedades inseticidas dos óleos essenciais são geralmente reconhecidas ao longo dos séculos e confirmadas cientificamente nas últimas décadas. Tais produtos podem ser usados na agricultura como uma alternativa para o controle de pragas. O óleo essencial derivado das folhas de Hymenaea courbaril L. var. courbaril foi obtido por hidrodestilação com arraste a vapor e caracterizado fitoquimicamente por cromatografia gasosa/espectrometria de massa (CG/EM); seu potencial acaricida e inseticida foi avaliado contra Tetranychus urticae e Sitophilus zeamais, respectivamente, pragas que ocorrem em uma ampla variedade de culturas economicamente importantes em todo o mundo. Após a análise por CG/EM, foram identificados 36 compostos, sendo 46,42% dos monoterpenos e 51,41% dos sesquiterpenos. Os bioensaios inseticidas avaliaram a interferência do óleo essencial na fisiologia nutricional do gorgulho e no processo de fumigação do ácaro. Os ensaios indicaram que o óleo essencial causou uma mortalidade significativa, promovendo danos fisiológicos ao S. zeamais. O valor de CL50 para T. urticae foi de 35,57 μL/mL de ar; além disso, também foi observado que a oviposição foi afetada negativamente pelo óleo essencial. O óleo essencial de H. courbaril demonstrou ser um potencial controle de pragas por afetar o metabolismo e a reprodução de ambas as espécies de praga testadas.

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Section: Discussionunclassified

Caracterização fitoquímica e avaliação do potencial acaricida e inseticida do óleo essencial de Hymeneae courbaril L. var. courbaril sobre o ácaro-rajado e o gorgulho do milho

Mercês¹,

Silva-Bessa²,

Malafaia

et al. 2018

J. Env. Anal. Progr.

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“…The soybean caterpillar Anticarsia gemmatalis Hübner (Lepidoptera: Erebidae) is the main defoliation pest of soybeans that can cause 100% of leaf loss (da Silva‐Júnior et al, 2020; Moscardi et al, 2012). The economic loss in soybean crops caused by the Lepidoptera insect A. gemmatalis has encouraged the search for strategies of pest control that are currently based on synthetic insecticides (Bueno, Carvalho, Santos, Sosa‐Gómez, & Silva, 2017; Krinski, Foerster, & Deschamps, 2018). However, new approaches are needed to reduce risks to the environment and natural enemies, and to avoid or delay the onset of insecticide resistance.…”

Section: Introductionmentioning

confidence: 99%

“…However, new approaches are needed to reduce risks to the environment and natural enemies, and to avoid or delay the onset of insecticide resistance. The use of plant‐based insecticides is an alternative for the control of lepidopteran pests, as they have low toxicity and short persistence in the environment (Gupta & Dikshit, 2010; Krinski et al, 2018). In this context, ApTI may be a promising alternative for the control of A. gemmatalis due to its inhibitory activity against serine proteases (trypsins and chymotrypsins) and cysteine proteases (papain) from insects.…”

Section: Introductionmentioning

confidence: 99%

Noncompetitive tight‐binding inhibition ofAnticarsia gemmatalistrypsins byAdenanthera pavoninaprotease inhibitor affects larvae survival

Meriño‐Cabrera

Mendes

Castro

et al. 2020

Arch Insect Biochem Physiol

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The economic loss in soybean crops caused by the Lepidoptera insects has encouraged the search for new strategies to control this pest, which are currently based on synthetic insecticides. This paper evaluated the ability of ApTI (Adenanthera pavonina trypsin inhibitor) to inhibit trypsin-like proteins from Anticarsia gemmatalis by docking, molecular dynamics, and enzymatic and survival assay. The docking and molecular dynamic simulation between trypsin and ApTI were performed using the program CLUSPRO and NAMD, respectively. The inhibitory constant K i and the inhibition type were determined through chromogenic assays. The survival assay of neonatal larvae under treatment with artificial diet supplemented with ApTI was also performed. The ApTI binding site was predicted to block substrate access to trypsin due to four interactions with the enzyme, producing a complex with a surface area of 1,183.7 Å 2 .The kinetic analysis revealed a noncompetitive tightbinding mechanism. The survival curves obtained using Kaplan-Meier estimators indicated that the highest larvae mortality was 60%, using 1.2 mg of ApTI per 100 ml of artificial diet. The in vitro, in vivo, and in silico studies demonstrated that ApTI is a strong noncompetitive inhibitor of trypsin with biotechnological potential for the control of A. gemmatalis insect. K E Y W O R D S binding, insects, Kunitz inhibitor, molecular docking, noncompetitive, trypsin enzyme

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“…In this sense, PIs can serve as pest control agents, either in the pulverized form or in transgenic plants that highly express these PIs (Meriño‐Cabrera et al, ; Pilon et al, ). Taking into account that the need for more appropriate formulations has raised the cost of agrochemicals over the years, interest in research into ecologically sound pesticides has increased (Krinski, Foerster, & Deschamps, ). This alternative method is cheaper in application, more specific, less polluting, and has a long shelf life because it is more difficult for a pest to become resistant (Pilon et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Currently, the control of agricultural pests tends towards reducing the use of agrochemicals due to their harmful effects on the environment and human health, being replaced by bioinsecticides that consider economic and ecological aspects (Chandler et al, ). Among the alternatives for the use of agrochemicals, the use of PI has been highlighted (Ferreira et al, ; Krinski et al, ).…”

Section: Introductionmentioning

confidence: 99%

Intestinal proteolytic profile changes during larval development of Anticarsia gemmatalis caterpillars

Junior

Vital

Barros

et al. 2019

Arch Insect Biochem Physiol

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Soybean is one of most consumed and produced grains in the world, and Anticarsia gemmatalis is a pest that causes great damage to this crop due to severe defoliation during its larval phase. Plants have mechanisms that lead to the inhibition of proteases in the intestine of these herbivores, hampering their development. Understanding this complex protease inhibitor is important for pest control. The objective of this study was to evaluate the enzymatic profiles of the intestinal proteases of the soybean caterpillar at different instars. For this, the proteolytic profile of the gut in the third, fourth, and fifth instars were analyzed.Irreversible inhibitors of proteases were separately incubated with A. gemmatalis enzyme extracts at the third, fourth, and fifth instar to assess the contribution of these proteases to total proteolytic activity. The enzymatic extracts were also evaluated with specific substrates to confirm changes in the specific activities of trypsin-like, chymotrypsin-like, and cysteine proteases at different instars. The results showed that the protease profile of A.gemmatalis gut changes throughout its larval development.The activity of cysteine proteases was more intense in the first instar. On the contrary, the serine proteases The protease profile of the gut of A. gemmatalis changes throughout larval development. In the third instar, cysteine protease activity is predominant over others. In the fourth and fifth instars, proteolytic activity is mainly due to serine proteases. This difference in activity in the different instars of A. gemmatalis suggests that the expression of the genes coding for proteases also changes throughout the development of the insect. Using serine PIs is a good strategy for controlling insect pests, as these are the main proteolytic enzymes of Lepidoptera. However, the presence of other important protease subclasses in insect digestibility suggests that a combination of serine and cysteine PIs may be promising, aiming to compromise larval development at different instars.

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<b>Ovicidal effect of the essential oils from 18 Brazilian <i>Piper</i> species: controlling <i>Anticarsia gemmatalis</i> (Lepidoptera, Erebidae) at the initial stage of development

Cited by 34 publications

References 65 publications

Caracterização fitoquímica e avaliação do potencial acaricida e inseticida do óleo essencial de Hymeneae courbaril L. var. courbaril sobre o ácaro-rajado e o gorgulho do milho

Caracterização fitoquímica e avaliação do potencial acaricida e inseticida do óleo essencial de Hymeneae courbaril L. var. courbaril sobre o ácaro-rajado e o gorgulho do milho

Noncompetitive tight‐binding inhibition ofAnticarsia gemmatalistrypsins byAdenanthera pavoninaprotease inhibitor affects larvae survival

Intestinal proteolytic profile changes during larval development of Anticarsia gemmatalis caterpillars

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