&lt;b&gt;Semen quality of Curimba (&lt;i&gt;Prochilodus lineatus&lt;/i&gt;) cryopreserved with vitamins&lt;/b&gt; - doi: 10.4025/actascitechnol.v36i1.19586

Navarro, Rodrigo Diana; Navarro, Fernanda Keley Silva Pereira; Felizardo, Viviane de Oliveira; Murgas, Luís David Solis; Andrade, Estefânia de Souza

doi:10.4025/actascitechnol.v36i1.19586

“…No significant differences were found between vitamin C and E for any of the parameters evaluated in this second stage, regardless of vitamin concentration (Table 3). Similar results were found by Cabrita et al (2001) and Navarro et al (2014) when using these same vitamins for the seminal cryopreservation of S. aurata and D. labrax, and P. lineatus, respectively. Regarding the concentrations employed, regardless of the vitamin used (Table 4), there was no significant difference in relation to sperm velocity and membrane integrity.…”

Section: Parâmetrossupporting

confidence: 79%

“…However, this vitamin did not influence the sperm motility rates of P. lineatus (NAVARRO et al, 2014), S. aurata or D. labrax (CABRITA et al, 2011;MARTÍNEZ-PÁRAMO et al, 2012). These results differ from those found for human semen (ASKARI et al, 1994) and boar (PENA et al, 2003), suggesting that this vitamin is more effective for mammals than for fish.…”

Section: Resultscontrasting

confidence: 56%

“…In view of the satisfactory results found, the study has created prospects for new investigations that evaluate both the association of both antioxidants used in the present research, other concentrations of these vitamins, as well as the use of other substances with the same properties. Navarro et al (2014) found no difference between vitamins C and E on the motility rate of P. lineatus when they used the concentration of 0.0001 mg. The same was observed by Cabrita et al (2011), who used concentrations of 1 and 10 mM of vitamin C and 0.1 and 0.5 mM of vitamin E for S. aurata.…”

Section: Parâmetrosmentioning

confidence: 99%

“…A number of studies have shown that these vitamins are used for seminal cryopreservation of Characiform fish (PAULA et al, 2012;NAVARRO et al, 2014), and there are no reports of their use for common curimatan seminal diluents. The objective of this study is to evaluate the association between two cryoprotectants and vitamins C and E, in different concentrations, on the quality of the cryopreserved semen of Prochilodus brevis.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei)

Almeida‐Monteiro

¹

,

Oliveira-Araújo

²

,

Lopes

³

et al. 2017

SCA

7

2

1

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Seminal cryopreservation allows the long-term conservation of gametes of various species, including endangered species, such as Prochilodus brevis. However, the application of this biotechnology can cause damage to sperm cells, reducing seminal quality. Thus, we have sought substances that minimize the damage caused by this process, such as antioxidants. Thus, this study aimed to evaluate the association between two cryoprotectants and two vitamins, in different concentrations, on the quality of cryopreserved semen of P. brevis. For cryopreservation, the experiment was performed in two stages. In the first stage, the semen of 10 animals was submitted to six different freezing means, coming from the combination of 5% glucose, two cryoprotectants (Dimethyl sulfoxide [DMSO] or Methyl glycol) and two vitamins (C or E to 0.0001 mg) for cryopreservation. In the second stage, semen samples of eight animals were diluted in 5% glucose and the best cryoprotectant found in the first stage, associated with three different concentrations of vitamins C or E (0.01, 0.001, and 0.0001 mg). In both steps, the in natura and post-thawed samples were submitted to kinetic analysis, morphology, and sperm membrane integrity. The cryopreserved semen with DMSO presented significantly higher results (p < 0.05) than that frozen with Methyl glycol, regardless of the vitamin used. The morphologically normal spermatozoa rate was higher (p < 0.05) in the vitamin-containing samples, however, vitamin E reduced sperm motility rates, independent of the cryoprotectant used. As for vitamin concentrations, higher motility rates were obtained when cryopreserved semen with 0.01 and 0.0001 mg of any of the vitamins. However, the higher concentration had a deleterious effect on the spermatic morphology of P. brevis. Therefore, the glucose associated with DMSO and the lower concentration of vitamin C provides good quality for the post-thawed semen of P. brevis. Key words: Antioxidants. Cryopreservation of semen. ResumoA criopreservação seminal permite a conservação em longo prazo dos gametas de diversas espécies, inclusive as ameaçadas, como a Prochilodus brevis. Contudo, a aplicação dessa biotecnologia pode causar danos às células espermáticas, reduzindo a qualidade seminal. Assim, tem-se buscado substâncias que minimizem os danos causados por esse processo, como os antioxidantes. Deste modo, este estudo objetivou avaliar a associação entre dois crioprotetores e duas vitaminas, em diferentes concentrações, sobre a qualidade do sêmen criopreservado de P. brevis. Para a criopreservação, o experimento foi realizado em duas etapas. Na primeira, o sêmen de 10 animais foi submetido a seis diferentes meios de congelação, oriundos da combinação de glicose 5%, dois crioprotetores (Dimetilsulfóxido [DMSO] ou Metilglicol) e duas vitaminas (C ou E a 0,0001 mg), para a criopreservação. Na segunda etapa, amostras de sêmen de oito animais foram diluídas em Glicose 5% e DMSO (melhor crioprotetor encontrado na primeira etapa), associados a três diferentes conce...

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“…Regarding the use of vitamins, in the present study, the addition of vitamin E to the freezing medium led to a decrease in the motility rate (Table 2), as observed by Liu et al (2015) for Pagrus major. However, this vitamin did not influence the sperm motility rates of P. lineatus (NAVARRO et al, 2014), S. aurata or D. labrax (CABRITA et al, 2011MARTÍNEZ-PÁRAMO et al, 2012). These results differ from those found for human semen (ASKARI et al, 1994) and boar (PENA et al, 2003), suggesting that this vitamin is more effective for mammals than for fish.…”

Section: Resultsmentioning

confidence: 91%

Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei)

Almeida‐Monteiro

¹

,

Oliveira-Araújo

²

,

Lopes

³

et al. 2017

SCA

View full text Add to dashboard Cite

Seminal cryopreservation allows the long-term conservation of gametes of various species, including endangered species, such as Prochilodus brevis. However, the application of this biotechnology can cause damage to sperm cells, reducing seminal quality. Thus, we have sought substances that minimize the damage caused by this process, such as antioxidants. Thus, this study aimed to evaluate the association between two cryoprotectants and two vitamins, in different concentrations, on the quality of cryopreserved semen of P. brevis. For cryopreservation, the experiment was performed in two stages. In the first stage, the semen of 10 animals was submitted to six different freezing means, coming from the combination of 5% glucose, two cryoprotectants (Dimethyl sulfoxide [DMSO] or Methyl glycol) and two vitamins (C or E to 0.0001 mg) for cryopreservation. In the second stage, semen samples of eight animals were diluted in 5% glucose and the best cryoprotectant found in the first stage, associated with three different concentrations of vitamins C or E (0.01, 0.001, and 0.0001 mg). In both steps, the in natura and post-thawed samples were submitted to kinetic analysis, morphology, and sperm membrane integrity. The cryopreserved semen with DMSO presented significantly higher results (p < 0.05) than that frozen with Methyl glycol, regardless of the vitamin used. The morphologically normal spermatozoa rate was higher (p < 0.05) in the vitamin-containing samples, however, vitamin E reduced sperm motility rates, independent of the cryoprotectant used. As for vitamin concentrations, higher motility rates were obtained when cryopreserved semen with 0.01 and 0.0001 mg of any of the vitamins. However, the higher concentration had a deleterious effect on the spermatic morphology of P. brevis. Therefore, the glucose associated with DMSO and the lower concentration of vitamin C provides good quality for the post-thawed semen of P. brevis.

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Criopreservación de semen de bagre rayado Pseudoplatystoma magdaleniatum con tres diferentes crioprotectores

Herrera-Cruz¹,

Aristizabal-Regino²,

Yepes-Blandón³

et al. 2019

Rev. colomb. biotecnol.

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El objetivo fue criopreservar semen de bagre rayado Pseudoplatystoma magdaleniatum con tres crioprotectores internos diferentes: dimetilsulfóxido (DMSO), dimetilacatamida (DMA) y etilenglicol (ETG) a dos porcentajes de inclusiones (5 y 10%), combinados con glucosa 6%, leche en polvo descremada 3% y vitamina E (0,4%). Cinco machos en fase de espermiación fueron inducidos con 0,4 ml de Ovaprim®/Kg. El semen fue diluido en la solución crioprotectora (1:3) en tubos de 2,5 ml, congelado en vapores de nitrógeno y descongelado a 35°C durante 90 segundos. El análisis estadístico incluyó un diseño factorial 3x2 y semen fresco (SF) como tratamiento testigo. En semen fresco, precongelado y descongelado se evaluó la movilidad total (Mt), tipos de movilidad, progresividad total y velocidades espermáticas con la ayuda de Sperm Class Analyzer SCA®. El SF registró volumen de 6,1±4,3 ml, Mt de 72,6±17,1%, activación de 31,2±2,1 segundos y concentración espermática de 54,7±22,9 millones/μl. En semen precongelado, el crioprotector (p<0,05) y porcentaje de inclusión (p<0,01), pero no su interacción, tuvieron un efecto significativo en la Mt, velocidad curvilínea (VCL) y velocidad lineal (VSL); mientras que en semen descongelado sólo la interacción de los factores (p<0,05) fue significativa en Mt, porcentajes de espermatozoide estáticos y VCL. La Mt cayó entre 36-67% en semen precongelado y entre 74-86% en semen descongelado con relación a SF. Los resultados sugieren que DMSO, DMA y ETG incluidos a 5 o 10%, combinados con leche en polvo 3%, glucosa 6% y vitamina E 0,4% son alternativas viables de criopreservación del semen de bagre rayado.

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<b>Semen quality of Curimba (<i>Prochilodus lineatus</i>) cryopreserved with vitamins</b> - doi: 10.4025/actascitechnol.v36i1.19586

Cited by 6 publications

References 21 publications

Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei)

Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei)

Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei)

Criopreservación de semen de bagre rayado Pseudoplatystoma magdaleniatum con tres diferentes crioprotectores

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