&lt;b&gt;The inhibitory effect of neonatal thymectomy on the incidence of insulitis in non-obese diabetes (NOD) &lt;/b&gt;&lt;b&gt;mice &lt;/b&gt;

Ogawa, Mikio; Murayama, Toshiyuki; Hasegawa, Toshiaki; Kanaya, Takashi; Kobayashi, Fuminori; Tochino, Yoshihiro; Uda, Hirotsugu

doi:10.2220/biomedres.6.103

Cited by 114 publications

(55 citation statements)

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“…The role of T cells in this model is shown by the predominance of T lymphocytes within the islet infiltrate (2,3), the preventive effect of neonatal thymectomy (4) and treatment with anti-CD4 (5) or anti-class II (6) monoslonal antibodies, and the induction of diabetes by transfer of T cells from diabetic NOD mice to nondiabetic B-cell-dOprived NOD recipients (7). Genetic studies have pointed to the role of major histocompatibility complex (MHC) genes in the susceptibility to diabetes (8,9).…”

mentioning

confidence: 99%

“…Purity was assessed by one-dimensional gel electrophoresis. Absorption of anti-peripherin autoantibody was performed by incubating 100 ,ul of a 1:50 dilution of 72-2 ascitic fluid (4 ,ug) with 250 ,u of purified peripherin (10 ,ug) Four other mouse anti-class II monoclonal antibodies with well-defined specificities did not react with NOD spleen cells and did not bind to Rin5F rat insulinoma cells. Importantly, the 58-kDa antigen recognized by anti-I-Anl' monoclonal antibodies was expressed by normal DBA/2 mouse islet cells.…”

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confidence: 99%

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Peripherin: an islet antigen that is cross-reactive with nonobese diabetic mouse class II gene products.

Boîtard

Villà

Bécourt

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

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The nonobese diabetic (NOD) mouse, in which major histocompatibility complex genes may be involved in the susceptibility to diabetes, has been developed as a model of autoimmune diabetes. The NOD mouse expresses I-A- (20) were grown in RPMI 1640 medium supplemented with 10o fetal calf serum, 2 mM glutamine, penicillin at 100 units/ml, and streptomycin at 100 jg/ml (GIBCO/BRL). Normal islets were prepared by collagenase (Sigma) digestion of mouse pancreases as described by Lacy and Kotianovsky (21) with slight modifications (22).Western Blots. Cells (1 x 108) from each cell line were washed in Hanks' balanced saline solution, suspended in 7 ml of 0.01M Tris-buffered saline (TBS)/10 mM CaCl2/0.25 M sucrose, pH 7.4, and disrupted at 40C. The cell homogenates were centrifuged (1000 x g, 10 min), and the supernatant was subjected to ultracentrifugation (20,000 x g, 1 h). The pellet was then resuspended in 2.3% SDS/62.5 mM Tris/10%6 glycerol/5% 2-mercaptoethanol, pH 6.8. One-dimensional gel electrophoresis and electrotransfers to nitrocellulose filters were performed as described by Laemmli (22) and Burnette (23). Two-dimensional SDS/PAGE was performed as described by O'Farrell (24). The filters were soaked in 0.02 M TBS/3% gelatin, washed in 0.05% Tween 20/0.02 M TBS, and then incubated with the test sera or ascitic fluid diluted 1:50 in 1% gelatin/0.05% Tween 20/0.02 M TBS. After washing, they were incubated with biotinylated sheep antiAbbreviations: NOD, nonobese diabetic; TBS, Tris-buffered saline; PBS, phosphate-buffered saline; NCS, newborn calf serum; MHC, major histocompatibility complex. tTo whom reprint requests should be addressed. 172The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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mentioning

confidence: 99%

mentioning

confidence: 99%

Peripherin: an islet antigen that is cross-reactive with nonobese diabetic mouse class II gene products.

Boîtard

Villà

Bécourt

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

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“…Thus, day-0 neonatal thymectomy prevents the onset of lupus in MRL-l mice [8], of diabetes in nonobese diabetic (NOD) mice [9], and of a number of experimentally induced autoimmune diseases (e.g. experimental allergic encephalomyelitis (EAE), experimental allergic thyroiditis or experimentally induced myasthenia gravis).…”

Section: ± the Polyautoimmune Syndrome Following Day-3 Thymectomymentioning

confidence: 99%

Non‐Th2 Regulatory T‐Cell Control of Th1 Autoimmunity

Bach

2001

Scand J Immunol

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The Th1/Th2 concept brought an attractive explanation of the active self tolerance which appears to control the onset of pathogenic autoimmunity. New data coming from various independent horizons indicate that self immunoregulation could also depend to a large extent on non‐Th2 cells. Original data derived from the day‐3‐thymectomy model, selective T‐cell lymphocytopenia and nonobese diabetic mice are discussed in an effort to analyze similarities and differences in phenotype (CD25, CD62L and CD45RB) and cytokine pattern (notably interleukin (IL)‐4, IL‐10 and transforming growth factor (TGF)β) of regulatory cells involved in these models. The relationship of these cells with Th3, Tr1 and natural killer (NK) T cells are also discussed. The hypothesis is proposed that CD25 CD62L T cells mediate the physiologic regulation of self regulation whereas Th2 and Th3 cells are essentially induced following sensitization against autoantigens.

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“…In studies using diabetic animals, T cells [2,3], natural killer cells [4] and macrophages [5][6][7] have been implicated in developing diabetes, but the mechanisms of direct beta-cell destruction…”

Section: Type 1(insulin-dependent)mentioning

confidence: 99%

Nitric Oxide Is Important for Mouse Beta-Cell Line Killing by Peritoneal Exudate Cells Obtained from Cyclophosphamide Treated Non-Obese Diabetic Mice.

et al. 1995

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Abstract. Macrophages from recent onset non-obese diabetic (NOD) mice showed cytotoxicity against the NOD mouse derived beta-cell line, MIN6N-9a. In this report, we examined whether nitric oxide is associated with beta-cell destruction. Peritoneal exudate cells (PEC), obtained from cyclophosphamide treated NOD mice showed higher cytotoxicity against MIN6N-9a compared to PECs from saline injected NOD mice (P<0.01). This effect was suppressed in cells incubated with 0.5 mmol/l NG-methyl-L-arginine, a nitric oxide synthase inhibitor (P<0.001). In addition, the nitrite concentration of the co-culture medium, as an index of nitric oxide production, increased in MIN6N-9a cells co-cultured with peritoneal exudate cells from cyclophosphamide injected NOD mice but not in co-culture with saline injected NOD mice (P<0.05). Thus, nitric oxide plays an important role in beta-cell line destruction of macrophages obtained from NOD mice.

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<b>The inhibitory effect of neonatal thymectomy on the incidence of insulitis in non-obese diabetes (NOD) </b><b>mice </b>

Cited by 114 publications

References 4 publications

Peripherin: an islet antigen that is cross-reactive with nonobese diabetic mouse class II gene products.

Peripherin: an islet antigen that is cross-reactive with nonobese diabetic mouse class II gene products.

Non‐Th2 Regulatory T‐Cell Control of Th1 Autoimmunity

Nitric Oxide Is Important for Mouse Beta-Cell Line Killing by Peritoneal Exudate Cells Obtained from Cyclophosphamide Treated Non-Obese Diabetic Mice.

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