&lt;b&gt;Use of agro-industrial wastes as substrates for α-amylase production by endophytic fungi isolated from &lt;i&gt;Piper hispidum&lt;/i&gt; Sw

Orlandelli, Ravely Casarotti; Santos, Manoel Luíz dos; Polônio, Julio Cesar; Azevedo, João Lúcio de; Pamphile, João Alencar

doi:10.4025/actascitechnol.v39i3.30067

Cited by 12 publications

(6 citation statements)

References 28 publications

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“…Since cellulases hydrolyze the plant cell wall during colonization by microbial endophytes (DUTTA et al, 2014), there is a great interest in endophytic sources of cellulase for industrial application. In fact, the search of microbial enzymes has already detected several endophytic sources of hydrolytic enzymes, as recently reported by Fouda et al (2015), Orlandelli et al (2015Orlandelli et al ( , 2017a and Ribeiro et al (2018).…”

Section: Introductionmentioning

confidence: 78%

Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates

Felber¹,

Specian²,

Orlandelli³

et al. 2019

Biosci. J.

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Endoglucanases are enzymes widely employed in different industrial fields, albeit with high production costs. Studies on new microbial sources and low-cost substrates are highly relevant, including those on agro-industrial. Current analysis evaluates peanut hull (PH) and sawdust (SD) as substrates for submerged cultures of 14 endophytic fungi isolated from grapevine (Vitis labrusca L.) cultivars Bordô and Concord. Endophytes were grown on a carboxymethylcellulose (CMC) medium and the cup plate assay showed that eight strains (belonging to genera Cochliobolus, Diaporthe, Fusarium and Phoma) had positive results: enzymatic halos ranged from 10.8±0.02 to 15.5±0.07 mm in diameter. Diaporthe sp. strains (GenBank accession codes KM362392, KM362368 and KM362378) and Fusarium culmorum KM362384 were highlighted as the most promising sources. Further, PH and SD as substrates for the fermentation of these fungi were evaluated by the cup plate assay and endoglucanase activity assay. Highest halo diameters were obtained for Diaporthe sp. KM362392: 16.1±0.01 mm (CMC), 14.5±0.01 mm (PH) and 14.7±0.03 mm (SD). The fungus also presented the highest levels of endoglucanase activity: analysis of variance revealed that CMC (3.52±0.98 µmol/min), PH (2.93±0.23 µmol/min) and SD (3.26±0.38 µmol/min) were similarly efficient as substrates. Results deepen knowledge on V. labrusca endophytes that may be endoglucanase sources, even though further optimizations in submerged cultures with PH and SD should be undertaken to increase the enzymatic production from these wastes.

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Section: Introductionmentioning

confidence: 78%

Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates

Felber¹,

Specian²,

Orlandelli³

et al. 2019

Biosci. J.

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“… Amylase, cellulase, lipase 61 Piper hispidum Sw Bipolaris sp. JF767001 α-amylase Orlandelli et al ( 2017 ) 62 Marasmius cladophyllus JF767003 α-Amylase 63 Phlebia sp. JF766997 α-Amylase 64 Phyllosticta capitalensis JF766988 α-amylase 65 Schizophyllum commune JF766994 α-amylase 66 Curcuma amada Talaromyces pinophilus KJ372306 l -Asparaginase Krishnapura et al ( 2016 ) 67 Bacopa monnieri Pleosporales sp.…”

Section: Research Progress On the Biodegradation Activity Of Endophytic Fungimentioning

confidence: 99%

Biotransformation ability of endophytic fungi: from species evolution to industrial applications

Liu

Zhou

Cui

et al. 2021

Appl Microbiol Biotechnol

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Increased understanding of the interactions between endophytic fungi and plants has led to the discovery of a new generation of chemical compounds and processes between endophytic fungi and plants. Due to the long-term co-evolution between fungal endophytes and host plants, endophytes have evolved special biotransformation abilities, which can have critical consequences on plant metabolic processes and their composition. Biotransformation or bioconversion can impact the synthesis and decomposition of hormones, sugars, amino acids, vitamins, lipids, proteins, and various secondary metabolites, including flavonoids, polysaccharides, and terpenes. Endophytic fungi produce enzymes and various bioactive secondary metabolites with industrial value and can degrade or sequester inorganic and organic small molecules and macromolecules (e.g., toxins, pollutants, heavy metals). These fungi also have the ability to cause highly selective catalytic conversion of high-value compounds in an environmentally friendly manner, which can be important for the production/innovation of bioactive molecules, food and nutrition, agriculture, and environment. This work mainly summarized recent research progress in this field, providing a reference for further research and application of fungal endophytes. Key points •The industrial value of degradation of endophytes was summarized. • The commercial value for the pharmaceutical industry is reviewed. Graphical abstract

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“…Dried cassava waste pulp containing moisture of 11.74%, starch content 59.40 %, ash content 1.95%, and crude fiber content 16.42%. The tempeh inoculum was identified and tested for amylase enzyme activity using the Fuwa method [16]. Macroscopic observations were made of fungal colonies' color growth on the petri dish.…”

Section: Characterizationmentioning

confidence: 99%

Synthesis L-Lactic Acid From Fermentation of Cassava Pulp by Using Tempeh Inoculum

Nissa

Sumiarsa

Kosasih³

et al. 2021

jsmi

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SYNTHESIS L-LACTIC ACID FROM FERMENTATION OF CASSAVA PULP BY USING TEMPEH INOCULUM. This study used cassava waste pulp as a fermentation substrate to produce lactic acid using a tempeh inoculum. Tempeh inoculum is a mixed culture of Rhizopus with Rhizopus oligosporus as the primary fungus. Lactic acid is an organic acid most widely used in the food, pharmaceutical, cosmetic and chemical industries. One of the important uses of lactic acid is as a raw material for producing Polylactic Acid (PLA) biopolymers, namely polymers that can decompose naturally in a relatively fast time. The analysis was performed using the Response Surface Methodology (RSM) method and the Box Behnken Design (BBD) experimental design with substrate concentration parameters, inoculum concentration, and incubation time on lactic acid. The fermentation process is carried out using a flask shaker at a temperature of 30 ºC, pH 6.0, and a rotational speed of 150 rpm. The optimum yield for lactic acid is 6.65 g/L. It was acquired at substrate 20 g/L, inoculum concentration 0.30 % (w/v) at an incubation time of 72 hours.

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<b>Use of agro-industrial wastes as substrates for α-amylase production by endophytic fungi isolated from <i>Piper hispidum</i> Sw

Cited by 12 publications

References 28 publications

Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates

Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. with peanut hull and sawdust as substrates

Biotransformation ability of endophytic fungi: from species evolution to industrial applications

Synthesis L-Lactic Acid From Fermentation of Cassava Pulp by Using Tempeh Inoculum

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