&lt;b&gt;Utilização das brânquias de Pimelodus maculatus (Lacèpéde, 1803) (Siluriformes; Pimelodidae) como biomarcador de poluição no reservatório da UHE Marechal Mascarenhas de Moraes, Minas Gerais, Brasil.&lt;/b&gt;&lt;br&gt;DOI: 10.5007/2175-7925.2011v24n3p51

Nogueira, Diego José; Castro, Sumaya Cardoso de; Vieira, Roberta Cristina Amâncio; Sá, Odila Rigolin de; Santos, Valter Monteiro de Azevedo

doi:10.5007/2175-7925.2011v24n3p51

Cited by 7 publications

(5 citation statements)

References 11 publications

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“…As modificações nos diferentes níveis biológicos (celulares, teciduais e sistêmicos) são indicativas dos danos que os contaminantes podem provocar nos peixes (Nogueira et al, 2008). Tais modificações são conhecidas como biomarcadores.…”

Section: Introductionunclassified

Biomarcadores histopatológicos na espécie Hoplias malabaricus (Pisces, Osteichthyes, Erythrinidae) em uma Unidade de Conservação de São Luís (MA)

Castro

Silva

Freitas

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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RESUMONeste estudo objetivou-se validar dois órgãos (brânquia e fígado) de Hoplias malabaricus para análise de biomarcadores de contaminação aquática na Área de Proteção Ambiental (APA) do Maracanã em São Luís-MA. Exemplares de traíra foram capturados em uma lagoa de policultivo e no Rio Ambude, localizados na APA do Maracanã. A biometria foi realizada em laboratório. Brânquias e o fígado de cada espécime foram fixados em formol a 10% e mantidos em álcool a 70% até a execução da técnica histológica usual. Cortes de aproximadamente 5µm de espessura foram corados em hematoxilina e eosina (HE). Os resultados referentes à biometria dos peixes capturados durante o período de estiagem indicaram que o comprimento total e padrão dos exemplares foram maiores quando comparados com os indivíduos do período chuvoso. As alterações hepáticas identificadas foram: lipidose, necrose, infiltração leucocitária, vacuolização do citoplasma, hemossiderina e centro de melanomacrófagos. Constataram-se as seguintes lesões branquiais: fusão lamelar, aneurisma lamelar, deslocamento e necrose do epitélio, proliferações de células do muco e dilatação capilar. As respostas biológicas de H. malabaricus, registradas em nível branquial e hepático, podem ser caracterizadas como biomarcadores de contaminação aquática. As brânquias mostraram-se como os melhores órgãos para esse tipo de análise na espécie. Esses resultados indicam que os peixes da região apresentam a saúde comprometida por poluentes presentes nos ambientes analisados. Palavras-chave: biomarcadores, lesões histológicas, monitoramento ambiental ABSTRACT The aim of the present work was to validate two organs (liver and gill) of Hoplias malabaricus for analysis of aquatic contamination biomarkers in the Maracanã Environmental Protected Area (APA) in

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Section: Introductionunclassified

Biomarcadores histopatológicos na espécie Hoplias malabaricus (Pisces, Osteichthyes, Erythrinidae) em uma Unidade de Conservação de São Luís (MA)

Castro

Silva

Freitas

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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“…Lamellar hyperplasia, fusion, and chloride cell hyperplasia have been widely observed in animals treated by immersion with EHFAo and are considered, according to Rigolim-Sá [46], as mechanisms of defense of the gills, which promote an increase of the blood-water barrier; it is considered an initial response of the gill apparatus, characterized by increased tissue cellular functions caused by changes in physiological activities. Although it is a defense mechanism, reducing or even totally hampering the passage of water between the secondary lamellae, this loss of respiratory surface can cause death by anoxia [28,[52][53][54][55].…”

Section: Discussionmentioning

confidence: 99%

Acute Toxicity of the Hydroethanolic Extract of the Flowers of Acmella oleracea L. in Zebrafish (Danio rerio): Behavioral and Histopathological Studies

et al. 2019

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Hydroethanolic preparations of the botanical species Acmella oleracea L. are used in the north of Brazil for the treatment of various diseases. However, few studies have been conducted to evaluate the toxicity of this species. The objective of this study was to evaluate the acute toxicity of the hydroethanolic extract of A. oleracea L. (EHFAo) flowers in zebrafish by immersion and oral administration. The extract was analyzed by ultra-performance liquid chromatography–mass spectrometry (UPLC–MS). EHFAo was administered orally (44.457, 88.915, 199.94, 281.83, and 448.81 mg/kg) and by immersion (250, 300, 350, 400, and 450 µg/L). Behavioral and histopathological analysis of gills, liver, intestine, and kidney were performed. The presence of (2E,6Z,8E)-N-isobutyl-2,6,8-decatrienamide (spilanthol) in EHFAo was identified by ultra-high-re.solution liquid chromatography–electrospray ionization mass spectrometry (UHPLC–ESI-MS). Treatment with EHFAo caused significant behavioral changes and death. The calculated median lethal dose (LD50) was 148.42 mg/kg, and the calculated median lethal concentration (LC50) was 320 μg/L. In the histopathological study, it was observed that upon oral treatment, the tissue alterations that compromised the normal functioning of the organism occurred with EHFAo doses of 88.915, 199.53, and 281.83 mg/kg, the intestine being the most affected. When the treatment was performed by immersion, the most toxic EHFAo concentrations according to the histopathological evaluation were 300, 350, and 400 μg/L, with the most affected organ being the gills. Finally, EHFAo in this study was shown to be more toxic to the liver, intestine, and kidneys when administered orally and to gills, liver, and kidneys when administered by immersion in water. Therefore, considering the results obtained and the chemical characteristics of the main phytochemical marker of EHFAo, spilanthol, it can be suggested that, depending on the dose, this compound can lead to histopathological damages in the organs highlighted in this study.

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“…These defense mechanisms are typical because they reduce or even entirely prevent the passage of water between the secondary lamellae. This loss of respiratory surface can cause death by anoxia [27][28][29].…”

Section: Discussionmentioning

confidence: 99%

Histopathology in Zebrafish (Danio rerio) to Evaluate the Toxicity of Medicine: An Anti-Inflammatory Phytomedicine with Janaguba Milk (Himatanthus drasticus Plumel)

Santos¹,

Souza²,

Santana³

et al. 2018

Histopathology - An Update

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The zebrafish Danio rerio appears to be as an alternative experimental model mainly used on toxicological evaluations since the 1990s. In this chapter, we illustrate using a histopathological study the evaluation of a complex phytopreparation with janaguba milk (TPJM, used in popular medicine), which was administrated in zebrafish by immersion in water. We determined (1) lethal concentration 50 (LC 50)-1188.54 μg/mL; (2) the behavioral changes; and (3) the acute administration of TPJM modifications (48 h) at concentrations 500, 750, 1000, and 1500 μg/mL, on the histopathological parameters of the gills, kidneys, and liver. Also the concentrations of 1000 and 1500 μg/mL caused significant damage to the gill tissue and produced a high rate of histological changes in the liver. The kidneys showed greater changes at concentrations of 750, 1000, and 1500 μg/mL. Based on the percentage of TPJM extracts that was only 1.85%, the LC 50 was calculated as 475 mg/kg; according to traditional indication, only 6 tablespoons/day is consumed; and it is possible to infer that only 0.5 g of active ingredient is ingested by an adult user per day, corresponding to a dose of 7.14 mg/kg, which is far from the toxic effects, demonstrating low toxicity of TPJM.

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<b>Utilização das brânquias de Pimelodus maculatus (Lacèpéde, 1803) (Siluriformes; Pimelodidae) como biomarcador de poluição no reservatório da UHE Marechal Mascarenhas de Moraes, Minas Gerais, Brasil.</b><br>DOI: 10.5007/2175-7925.2011v24n3p51

Cited by 7 publications

References 11 publications

Biomarcadores histopatológicos na espécie Hoplias malabaricus (Pisces, Osteichthyes, Erythrinidae) em uma Unidade de Conservação de São Luís (MA)

Biomarcadores histopatológicos na espécie Hoplias malabaricus (Pisces, Osteichthyes, Erythrinidae) em uma Unidade de Conservação de São Luís (MA)

Acute Toxicity of the Hydroethanolic Extract of the Flowers of Acmella oleracea L. in Zebrafish (Danio rerio): Behavioral and Histopathological Studies

Histopathology in Zebrafish (Danio rerio) to Evaluate the Toxicity of Medicine: An Anti-Inflammatory Phytomedicine with Janaguba Milk (Himatanthus drasticus Plumel)

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