<em>ITGA7 </em>functions as a tumor suppressor and regulates migration and invasion in breast cancer

Abstract

BackgroundBreast cancer is the most common malignancy in women and the underlying mechanism of breast cancer cell metastasis is still far from uncover. Integrin subunit alpha 7 (ITGA7) is a functioning protein. It has been detected in many malignancies. But the function of ITGA7 in breast cancer is not clear. Our aim is to explore ITGA7 expression and its role in breast cancer.MethodsReal-time PCR was performed to determine ITGA7 expression in BC tissues and normal adjacent tissues. The specific functions of ITGA7 in breast cancer cell lines (MDA-MB-231 and BT-549) transfected with small interfering RNA were determined through migration, invasion assays. Western blot assays were performed to determine the expression of c-met and vimentin.ResultsITGA7 was down-regulated in breast cancer tissues compared to the adjacent normal tissues (T:N =7.68±27.38: 41.01± 31.47, P<0.001) and this observation was consistent with the TCGA cohort (T:N =4.51±0.45:5.40±0.61, P<0.0001). In vitro experiments showed that knocking down ITGA7 significantly inhibited the migration and invasion of the breast cancer cell lines (MDA-MB-231 and BT-549). Meanwhile, knockdown of ITGA7 promoted c-met and vimentin expression, which may induce invasion and migration.ConclusionITGA7 plays an important tumorigenic function and acts as a suppress gene in breast cancer. Our findings indicate that ITGA7 was the gene associated with breast cancer.

Downregulated hsa_circ_0077837 and hsa_circ_0004826, facilitate bladder cancer progression and predict poor prognosis for bladder cancer patients

“…The circ_0026782, circ_0001946, circ_0004826, circ_0077837, circ_0030586, circ_0008039, circ_0001346, and circ_0003141 are spliced from ITGA7, CDR1, UTRN, EPB41L2, ABCC4, PRKAR1B, RNF13, and UBAP2, which all play an important effect in tumor proliferation, migration, and metastasis. Bhandari A et al reported that ITGA7 acts as a tumor suppressor and regulates migration and invasion in breast cancer 52 . According to cell proliferation assays as well as the mouse xenograft model, Li et al determined UTRN as a breast cancer suppressor gene both in vitro and in vivo 53 .…”

Stilbenoid‐Mediated Epigenetic Activation of Semaphorin 3A in Breast Cancer Cells Involves Changes in Dynamic Interactions of DNA with DNMT3A and NF1C Transcription Factor

“…For instance, hypermethylated SERPINB12 protease inhibitor, located in the serpin gene cluster on chromosome 18, is downregulated in clinical samples which suggests its potential tumor suppressor role . Hypermethylated ITGA7 belongs to the integrin family and is a newly identified tumor suppressor gene that decreases migration and invasion of cancer cells . On the other hand, hypomethylated MMP1 is a potent oncogene promoting metastasis and multi‐drug resistance, and hypomethylated PLEKHA5 facilitates metastasis to the brain .…”

lncRNA FOXD3‐AS1 is associated with clinical progression and regulates cell migration and invasion in breast cancer

“…A lot of studies have discovered many oncogenes and tumour suppressor genes, which are acknowledged to be related to the occurrence of breast cancer. [7][8][9] Long noncoding RNAs as a heterogeneous class of noncoding RNAs, which are longer than 200 nucleotides, have been identified to involve in many human diseases, including tumorigenesis. 10,11 A few long noncoding RNAs (lncRNAs) have been identified as oncogenic or suppressor genes and regulate the progress of breast cancer.…”

“…[7][8][9] Long noncoding RNAs as a heterogeneous class of noncoding RNAs, which are longer than 200 nucleotides, have been identified to involve in many human diseases, including tumorigenesis. In recent years, increasing long noncoding RNA (lncRNA) has been discovered to be related to tumorigenesis, progression, and prognosis.…”