&lt;em&gt;SFTPC&lt;/em&gt; genetic polymorphisms are associated with tuberculosis susceptibility and clinical phenotype in a Western Chinese Han population

Zhao, Junwei; Jiao, Lin; Guo, Meng; Wang, Xuebin; Gao, Shanxing; Ying, Binwu; Ming, Liang

doi:10.3892/etm.2020.9230

Cited by 4 publications

(4 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…SFTPA and SFTPD also regulate the functions of dendritic cells and T cells (68). Sftpa, Sftpd, and Sftpc gene polymorphisms not only increase the risk of TB but also may affect the host's immune response to MTB (69). Thacker VV et al (70) showed that the decreased expression of alveolar epithelial cells type II markers (Abca3, Sftpa, Sftpb, Sftpc, Sftpd) and type I markers (Aqp5 and Pdpn) would lead to the rapid growth of MTB in macrophages and alveolar epithelial cells (71).…”

Section: Discussionmentioning

confidence: 99%

Immunotherapeutic mechanisms of ag85a/b DNA vaccine and its recovery effect on M. tuberculosis-induced injury

Wang,

Wu,

Liang

et al. 2024

Preprint

View full text Add to dashboard Cite

Our previous research developed a novel tuberculosis (TB) DNA vaccine ag85a/b showed a significant therapeutic effect on the mouse tuberculosis model by intramuscular injection (IM) and electroporation (EP). However, the action mechanisms between these two vaccine immunization methods remain unclear. In a previous study, 96 M. tuberculosis (MTB) H37Rv-infected BALB/c mice were treated with PBS, 10μg, 50μg, 100μg, and 200μg ag85a/b DNA vaccine delivered by IM and EP three times at two-week intervals, respectively. In this study, peripheral blood mononuclear cells (PBMCs) from 3 mice in each group were isolated to extract total RNA. The gene expression profiles were analyzed using gene microarray technology to obtain differentially expressed (DE) genes. Finally, DE genes were validated by real-time reverse transcription-quantitive PCR (RT-qPCR) and the GEO database. After MTB infection, most of the up-regulated DE genes were related to the digestion and absorption of nutrients or neuroendocrine, for example, Iapp, Scg2, Chga, Amy2a5, etc, and most of the down-regulated DE genes were related to cellular structural and functional proteins, especially the structure and function proteins of alveolar epithelial cell, for example, Sftpc, Sftpd, Pdpn, etc. Most of the abnormally up-regulated or down-regulated DE genes in the TB model group were recovered in the 100μg and 200μg ag85a/b DNA IM groups and four DNA EP groups. The pancreatic secretion pathway down-regulated and Rap1 signal pathway up-regulated had particularly significant changes during the immunotherapy of the ag85a/b DNA vaccine on the mouse TB model. The action target and mechanism of IM and EP are highly consistent. Tuberculosis infection caused rapid catabolism and slow anabolism in mice. For the first time, we found that the effective dose of the ag85a/b DNA vaccine immunized whether by IM or EP could significantly up-regulate immune-related pathways and recover the metabolic disorder and the injury caused by MTB.

show abstract

Section: Discussionmentioning

confidence: 99%

Immunotherapeutic mechanisms of ag85a/b DNA vaccine and its recovery effect on M. tuberculosis-induced injury

Wang,

Wu,

Liang

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…SFTPA and SFTPD also regulate the functions of dendritic cells and T cells 104 . Sftpa, Sftpd, and Sftpc gene polymorphisms not only increase the risk of TB but also may affect the host's immune response to MTB 105 . Thacker VV et al 106 showed that the decreased expression of alveolar epithelial cells type II markers (Abca3, Sftpa, Sftpb, Sftpc, Sftpd) and type I markers (Aqp5 and Pdpn) would lead to the rapid growth of MTB in macrophages and alveolar epithelial cells 107 .…”

Section: Discussionmentioning

confidence: 99%

“… 104 Sftpa, Sftpd, and Sftpc gene polymorphisms not only increase the risk of TB but also may affect the host's immune response to MTB. 105 Thacker VV et al 106 showed that the decreased expression of alveolar epithelial cells type II markers (Abca3, Sftpa, Sftpb, Sftpc, Sftpd) and type I markers (Aqp5 and Pdpn) would lead to the rapid growth of MTB in macrophages and alveolar epithelial cells. 107 The growth of MTB in these two cells could be inhibited by the exogenous addition of Curosurf (surfactant substitute of phospholipid and hydrophobic protein).…”

Section: Discussionmentioning

confidence: 99%

Mechanisms of ag85a/b DNA vaccine conferred immunotherapy and recovery from Mycobacterium tuberculosis‐induced injury

Wang,

Liang,

et al. 2023

Immunity Inflam & Disease

View full text Add to dashboard Cite

Our previous research developed a novel tuberculosis (TB) DNA vaccine ag85a/b that showed a significant therapeutic effect on the mouse tuberculosis model by intramuscular injection (IM) and electroporation (EP). However, the action mechanisms between these two vaccine immunization methods remain unclear. In a previous study, 96 Mycobacterium tuberculosis (MTB) H37Rv‐infected BALB/c mice were treated with phosphate‐buffered saline, 10, 50, 100, and 200 μg ag85a/b DNA vaccine delivered by IM and EP three times at 2‐week intervals, respectively. In this study, peripheral blood mononuclear cells (PBMCs) from three mice in each group were isolated to extract total RNA. The gene expression profiles were analyzed using gene microarray technology to obtain differentially expressed (DE) genes. Finally, DE genes were validated by real‐time reverse transcription‐quantitive polymerase chain reaction and the GEO database. After MTB infection, most of the upregulated DE genes were related to the digestion and absorption of nutrients or neuroendocrine (such as Iapp, Scg2, Chga, Amy2a5), and most of the downregulated DE genes were related to cellular structural and functional proteins, especially the structure and function proteins of the alveolar epithelial cell (such as Sftpc, Sftpd, Pdpn). Most of the abnormally upregulated or downregulated DE genes in the TB model group were recovered in the 100 and 200 μg ag85a/b DNA IM groups and four DNA EP groups. The pancreatic secretion pathway downregulated and the Rap1 signal pathway upregulated had particularly significant changes during the immunotherapy of the ag85a/b DNA vaccine on the mouse TB model. The action targets and mechanisms of IM and EP are highly consistent. Tuberculosis infection causes rapid catabolism and slow anabolism in mice. For the first time, we found that the effective dose of the ag85a/b DNA vaccine immunized whether by IM or EP could significantly up‐regulate immune‐related pathways and recover the metabolic disorder and the injury caused by MTB.

show abstract

“…Установлено, что легочный сурфактант, наряду с участием в процессе осуществления дыхания обеспечивает молекулярные механизмы врожденного и приобретенного иммунитета легких и обладает противовоспалительными свой ствами [3,4,6,7,8,10,14,15,16,17,19,21,22]. Дефицит сурфактанта, вызванный гибелью альвеолоцитов II типа его синтезирующих, рассматривается как ведущая причина коллабирования альвеол с развитием микроателектазирования и тяжелого нарушения вентиляционно-перфузионных отношений.…”

Section: Introductionunclassified

Inhalation Surfactant Therapy within Comprehensive Treatment of HIV-Infected Patients with Multiple Drug Resistant Tuberculosis

Zhukova,

Stavitskaya,

Pushkareva

et al. 2023

Tuberk. bolezni lëgk.

View full text Add to dashboard Cite

The objective: to evaluate the main eff ects of surfactant inhalations during the intensive phase of chemotherapy with the regimen containing bedaquiline and linezolid in HIV-infected patients with multiple drug resistant tuberculosis.Subjects and Methods. A single-center, open-label, controlled prospective cohort study was conducted. 80 patients with MDR-TB/HIV were enrolled in the study and randomized into two groups: ST+ Group included 40 patients who in addition to anti-tuberculosis therapy containing bedaquiline and linezolid during the intensive phase received inhalation of the suspension of Surfactant-BL according to a certain regimen. The course of 28 inhalations took 2 months, the total dose of surfactant was 700 mg. ST- Group included 40 patients who received anti-tuberculosis therapy only. To assess the severity of respiratory symptoms in patients, we used our own scoring. All patients also received antiretroviral therapy.Results. In the ST+ Group versus ST- Group, there was a decrease in the severity of tuberculosis clinical manifestations and timing of their relief, higher frequency of cavity healing by 21.2%, and faster sputum conversion by 28.6%. These rates are higher than in ST- Group and this is a short period of 10 weeks. None of the patients developed adverse reactions to surfactant inhalations. Patients tolerated well the combination of surfactant therapy, MDR TB chemotherapy containing bedaquiline and linezolid, and ART.

show abstract

<em>SFTPC</em> genetic polymorphisms are associated with tuberculosis susceptibility and clinical phenotype in a Western Chinese Han population

Cited by 4 publications

References 32 publications

Immunotherapeutic mechanisms of ag85a/b DNA vaccine and its recovery effect on M. tuberculosis-induced injury

Immunotherapeutic mechanisms of ag85a/b DNA vaccine and its recovery effect on M. tuberculosis-induced injury

Mechanisms of ag85a/b DNA vaccine conferred immunotherapy and recovery from Mycobacterium tuberculosis‐induced injury

Inhalation Surfactant Therapy within Comprehensive Treatment of HIV-Infected Patients with Multiple Drug Resistant Tuberculosis

Contact Info

Product

Resources

About