&lt;em&gt;Vibrio fischeri&lt;/em&gt; and &lt;em&gt;Escherichia coli&lt;/em&gt; adhesion tendencies towards photolithographically modified nanosmooth poly (&lt;em&gt;tert&lt;/em&gt;-butyl methacrylate) polymer surfaces

Ivanova, Elena P.

doi:10.2147/nsa.s3571

Cited by 7 publications

(5 citation statements)

References 49 publications

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“…It is believed that EPS production is essential in facilitating the initial stages of bacterial attachment to various surfaces (8,13). Our recent studies on bacterial attachment to nanosmooth glass surfaces and to poly(tert-butyl methacrylate) (P(tBMA)) polymeric surfaces were in agreement with this concept: Enhanced bacterial attachment was accompanied by elevated levels of secreted extracellular polymeric materials (9,10,13). In this study the shift in production of low amounts of EPS might be also due to the low carbon/nitrogen ratio as it was previously reported that EPS production is strongly dependent on the carbon/nitrogen ratio of the nutrient medium (5).…”

Section: Discussionsupporting

confidence: 59%

Poly(ethylene terephthalate) Polymer Surfaces as a Substrate for Bacterial Attachment and Biofilm Formation

et al. 2009

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Plastic debris causes extensive damage to the marine environment, largely due to its ability to resist degradation. Attachment on plastic surfaces is a key initiation process for their degradation. The tendency of environmental marine bacteria to adhere to poly(ethylene terephthalate) (PET) plastic surfaces as a model material was investigated. It was found that the overall number of heterotrophic bacteria in a sample of sea water taken from St. Kilda Beach, Melbourne, Australia, was significantly reduced after six months from 4.2-4.7×10 3 cfu mL −1 to below detectable levels on both full-strength and oligotrophic marine agar plates. The extinction of oligotrophs after six months was detected in all samples. In contrast, the overall bacterial number recovered on full strength marine agar from the sample flasks with PET did not dramatically reduce. Heterotrophic bacteria recovered on full-strength marine agar plates six months after the commencement of the experiment were found to have suitable metabolic activity to survive in sea water while attaching to the PET plastic surface followed by the commencement of biofilm formation.

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Section: Discussionsupporting

confidence: 59%

Poly(ethylene terephthalate) Polymer Surfaces as a Substrate for Bacterial Attachment and Biofilm Formation

et al. 2009

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“…The largely pH-independent ζ-potential values measured in this work (Table 1) are in good agreement with the −30.1 ± 9 mV previously reported for V. fischeri (DSM 507 strain) at pH 7.2 [59]. In particular, the near-constant surface charge observed for V. fischeri cells grown in the media at pH 6, 7, or 8, i.e., under conditions analogous to those of the long-term cell-growth and bioluminescence measurements in Figure 1, implies that the suppressed growth at pH 6 did not result from dramatic changes in the electrostatic environment of cell surfaces.…”

Section: Surface Charge Of V Fischeri Is Ph-independentsupporting

confidence: 91%

“…The observed variations in the mean diameter values were not statistically significant among the timepoints, with an overall mean diameter of 0.6 ± 0.1 µm. The mean length observed in this work was at the lower and upper limits, respectively, of the previous literature values for V. fischeri cells measured using optical microscopy (2-3 µm) [27,32] and SEM (1.5-2 µm) [59]. The overall larger values obtained using optical microscopy were likely due to the shrinking of bacterial cells that commonly results from their preparation for SEM imaging, which includes fixation with glutaraldehyde and dehydration in ethanol and in the critical-point dryer [61].…”

Section: Typical Morphology Of V Fischeri Cellssupporting

confidence: 68%

pH-Induced Modulation of Vibrio fischeri Population Life Cycle

et al. 2021

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Commonly used as biological chemosensors in toxicity assays, Vibrio fischeri bacteria were systematically characterized using complementary physicochemical and biological techniques to elucidate the evolution of their properties under varying environmental conditions. Changing the pH above or below the optimal pH 7 was used to model the long-term stress that would be experienced by V. fischeri in environmental toxicology assays. The spectral shape of bioluminescence and cell-surface charge during the exponential growth phase were largely unaffected by pH changes. The pH-induced modulation of V. fischeri growth, monitored via the optical density (OD), was moderate. In contrast, the concomitant changes in the time-profiles of their bioluminescence, which is used as the readout in assays, were more significant. Imaging at discrete timepoints by scanning electron microscopy (SEM) and helium-ion microscopy (HIM) revealed that mature V. fischeri cells maintained a rod-shaped morphology with the average length of 2.2 ± 1 µm and diameter of 0.6 ± 0.1 µm. Detailed morphological analysis revealed subpopulations of rods having aspect ratios significantly larger than those of average individuals, suggesting the use of such elongated rods as an indicator of the multigenerational environmental stress. The observed modulation of bioluminescence and morphology supports the suitability of V. fischeri as biological chemosensors for both rapid and long-term assays, including under environmental conditions that can modify the physicochemical properties of novel anthropogenic pollutants, such as nanomaterials and especially stimulus-responsive nanomaterials.

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“…As the interaction with the cellular membrane seems to be the main factor that explains the biological activity of CFA and its Eu(III) complexes, a detailed study was performed in order to obtain further insight into the compound–membrane interactions. This study was performed by electrophoretic mobility, a commonly used technique for surface characterization of nonbiological colloids and, more recently, bacterial, yeast or animal cells [ 73 ]. The determination of the surface charge of living cells ( δ ) is of prominent importance for understanding their behaviour and functions under various environmental conditions.…”

Section: Resultsmentioning

confidence: 99%

Caffeic Acid/Eu(III) Complexes: Solution Equilibrium Studies, Structure Characterization and Biological Activity

Arciszewska

Gama

Kalinowska

et al. 2022

IJMS

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Caffeic acid (CFA) is one of the various natural antioxidants and chemoprotective agents occurring in the human diet. In addition, its metal complexes play fundamental roles in biological systems. Nevertheless, research on the properties of CFA with lanthanide metals is very scarce, and little to no chemical or biological information is known about these particular systems. Most of their properties, including their biological activity and environmental impact, strictly depend on their structure, stability, and solution behaviour. In this work, a multi-analytical-technique approach was used to study these relationships for the Eu(III)/CFA complex. The synthesized metal complex was studied by FT-IR, FT-Raman, elemental, and thermal (TGA) analysis. In order to examine the chemical speciation of the Eu(III)/CFA system in an aqueous solution, several independent potentiometric and spectrophotometric UV-Vis titrations were performed at different M:L (metal:ligand) and pH ratios. The general molecular formula of the synthesized metal complex in the solid state was [Eu(CFA)3(H2O)3]∙2H2O (M:L ratio 1:3), while in aqueous solution the 1:1 species were observed at the optimum pH of 6 ≤ pH ≤ 10, ([Eu(CFA)] and [Eu(CFA)(OH)]−). These results were confirmed by 1H-NMR experiments and electrospray-ionization mass spectrometry (ESI-MS). To evaluate the interaction of Eu(III)/CFA and CFA alone with cell membranes, electrophoretic mobility assays were used. Various antioxidant tests have shown that Eu(III)/CFA exhibits lower antioxidant activity than the free CFA ligand. In addition, the antimicrobial properties of Eu(III)/CFA and CFA against Escherichia coli, Bacillus subtilis and Candida albicans were investigated by evaluation of the minimum inhibitory concentration (MIC). Eu(III)/CFA shows higher antibacterial activity against bacteria compared to CFA, which can be explained by the highly probable increased lipophilicity of the Eu(III) complex.

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<em>Vibrio fischeri</em> and <em>Escherichia coli</em> adhesion tendencies towards photolithographically modified nanosmooth poly (<em>tert</em>-butyl methacrylate) polymer surfaces

Cited by 7 publications

References 49 publications

Poly(ethylene terephthalate) Polymer Surfaces as a Substrate for Bacterial Attachment and Biofilm Formation

Poly(ethylene terephthalate) Polymer Surfaces as a Substrate for Bacterial Attachment and Biofilm Formation

pH-Induced Modulation of Vibrio fischeri Population Life Cycle

Caffeic Acid/Eu(III) Complexes: Solution Equilibrium Studies, Structure Characterization and Biological Activity

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