&lt;i&gt;Agrobacterium&lt;/i&gt;-mediated Genetic Transformation of Potato (&lt;i&gt;Solanum tuberosum&lt;/i&gt; L.) var. Cardinal and Heera

Khatun, Asma; Hasan, M. M.; Bachchu, Md. Adnan Al; Moniruzzaman, M.; Nasiruddin, KM

doi:10.3329/agric.v10i1.11068

Cited by 8 publications

(6 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…So we tried another infection time (5min) 3-days co-cultivation and some explants survive and then transferred for cultivation MS media. This agree with [17] who used 5-min infection into two potato cultivars from Bangladesh (cardinal and heera), [31], from Molecular Plant Pathology Laboratory in USA, who worked on two Serbian potato cultivars (Dragaevka and Jelica) and [32] To sum up, the difference in infection periods of explants with Agrobacterium could be due to firstly, used different cultivars which could be differ in their sensitivity to infection with bacteria. Secondly it may be due to use different explants for infection (node, internode or leaf segment), finally it could be due to different environmental conditions which completely different differ from the environmental conditions in Egypt.In this study, we selectednodal explants for transformation because [23] proved that the nodal explants are much more resistant during manipulation and more amenable to in vitro conditions.…”

Section: Discussionsupporting

confidence: 64%

“…The mechanism of thionins inhibition to the pathogenic fungi was simply explained by [16] as follow: the antimicrobial properties of thionins are derived from their ability to induce pore formation on cell membranes of phytopathogens, resulting in release of potassium and calcium ions from the cell. [17]mentioned that Agrobacterium-mediated transformation has become feasible. It is the most common method used for the genetic transformation of potato.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transgenic Potato (Solanumtuberosum) Expressing Two Antifungal Thionin Genes Confer Resistance To Fusarium Spp.

Hammad¹,

Abdel-Razik²,

Soliman³

et al. 2017

IOSRJPBS

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Transgenic Potato (Solanumtuberosum) Expressing Two Antifungal Thionin Genes Confer Resistance To Fusarium Spp.

Hammad¹,

Abdel-Razik²,

Soliman³

et al. 2017

IOSRJPBS

View full text Add to dashboard Cite

show abstract

“…reported for nodal and internal explants (Borna et al 2010, Hussain et al 2005, Sarker and Mustafa 2002, Philip and Hampson 1995, while for leaf and internodes, NAA and BAP were reported to be the best (Khatun et al 2012). Both studies are different from our observation.…”

Section: Resultscontrasting

confidence: 56%

Micropropagation of Environmental Stress Tolerant Local Potato (Solanum tuberosum L.) Varieties of Bangladesh

Parveen

Khatun

Islam

2014

Plant Tissue Cult. & Biotech.

View full text Add to dashboard Cite

In vitro response of four explants namely, leaf, shoot apex, nodal and internodal, in three stress tolerant Bangladeshi potato varieties, viz. Zaubilati, Shadaguti and Challisha were tested. Of all the varieties, Shadaguti responded best for all the explants. Among the explants nodal segment responded best, followed by shoot apex. For all these explants and varieties, shoot regeneration response was tested in response to two cytokines, BAP and Kn. When compared between BAP and Kn supplementation, Kn treatment performed better than that of BAP for nodal and shoot apex, while opposite was observed for remaining explants. Interestingly, hormone free basal PROP medium was found to be best for nodal explants of both Zaubilati and Challisha varieties. While nodal explants of Shadaguti showed the best result in 0.5 mg/l Kn. Shoot apex performed best in 0.5 mg/l Kn for all the varieties. As an explant both internode and leaf did not perform well. Direct regeneration from these explants was found best in PROP + 0.2 mg/l GA3 + 0.5 mg/l IAA; 1.0 mg/l BAP, 1.5 mg/l BAP and 2.0 mg/l BAP for Zaubilai, Challisha and Shadaguti, respectively. For rooting, of the in vitro grown shoots half strength of PROP medium supplemented with 0.5 mg/l IBA was found to be best. Cent percent survival of transplanted plantlets was recorded. The successful protocol for in vitro regeneration was developed which can be used for large scale production of these abiotic stress tolerant potato varieties.

show abstract

“…and the GA 3 also had different concentration in different species. The results of others research also indicated that the hormone ratio required for different genotypes of potato to differentiate into shoots was different, and the differentiation efficiency was similarly different [21][22][23][24][25].…”

Section: Selection Of Shoots Induction Medium For Callusmentioning

confidence: 99%

Construction of Potato DM1-3-516-R44 (DM) Transgenic System Based on Agrobacterium Transformation

Zhang

Wang

Yang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The sequencing potato DM1-3-516-R44 played an irreplaceable role in the study of gene function. So far, no one research the transformation system about DM. Therefore, our experiment was studied from three aspects: plant regeneration system, optimization of agrobacterium infection conditions and the effect of hygromycin on DM. Results: A relatively suitable method for genetic transformation of DM was obtained: 1) The stem callus induction medium was MS + IAA 0.5 mg/L + 6-BA 2.0 mg/L, the leaf callus induction medium was MS + NAA 1.0mg/L + 6-BA 0.5mg/L and the shoot differentiation medium was MS + 6-BA 3.0 mg/L + ZT 0.5 mg/L. 2) The specific transformation condition was the agrobacterium concentration kept the OD600 = 0.3, and co-culture time consisted 3 days in the dark. The hygromycin concentration chose 8 mg/L to screen the transgenic plants. 3) Using hygromycin to screen about 100 transgenic shoots, 75 shoots were obtained and 53 strains were identified had target stripe by PCR technology. Conclusion: The efficiency of the transformation system we created was over 50%. It provided a good basis for the study of potato gene function.

show abstract

<i>Agrobacterium</i>-mediated Genetic Transformation of Potato (<i>Solanum tuberosum</i> L.) var. Cardinal and Heera

Cited by 8 publications

References 4 publications

Transgenic Potato (Solanumtuberosum) Expressing Two Antifungal Thionin Genes Confer Resistance To Fusarium Spp.

Transgenic Potato (Solanumtuberosum) Expressing Two Antifungal Thionin Genes Confer Resistance To Fusarium Spp.

Micropropagation of Environmental Stress Tolerant Local Potato (Solanum tuberosum L.) Varieties of Bangladesh

Construction of Potato DM1-3-516-R44 (DM) Transgenic System Based on Agrobacterium Transformation

Contact Info

Product

Resources

About