&lt;i&gt;bcl-2&lt;/i&gt; Expression and Apoptosis in Primary and Metastatic Breast Carcinomas

Villar, Eduardo; Redondo, Maximino; Rodrigo, Isabel; Garcia, J F; Avila, E. Martinez; Matilla, Alfredo

doi:10.1159/000050608

Cited by 29 publications

(22 citation statements)

References 30 publications

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“…In fact, p53 overexpression has been related to a high AI in breast [16,35] and ovarian carcinomas [36]. However, it should also be considered that in the present study p53 is related to proliferation and that proliferating tumors present high levels of apoptotic activity [37]. Thus, the close correlation between p53 and proliferation is in agreement with other reports [29,38].…”

Section: Discussionsupporting

confidence: 92%

Expression of bax and p53 Proteins in the Tumorigenesis and Progression of Breast Carcinomas

Redondo¹,

Garcia²,

Rodrigo³

et al. 2003

Tumor Biol

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Objectives: Dysregulation of normal programmed cell death mechanisms plays an important role in the pathogenesis of breast cancer. The purpose of this study was to investigate the role of bax and p53 expression in tumorigenesis and progression of breast carcinoma as well as their relationship with proliferative and apoptotic activity. Methods: We used immunohistochemical methods and in situ detection of apoptotic cells to examine 30 carcinomas in situ (CIS), 131 invasive breast carcinomas and 45 lymph node metastases. Results: In 25% (33 of 131) of invasive breast carcinomas examined, bax expression was absent, while p53 accumulation was present in 37% (49 of 131). Interestingly, p53 accumulation and loss of bax expression occur in breast CIS as frequently as in invasive breast carcinoma. Thus, in 17% (5 of 30) of CIS bax expression was absent, and 30% (9 of 30) presented nuclear expression of p53. p53 accumulation was related to apoptosis and proliferative activity. However, the protein level of bax was unrelated to all parameters studied, including proliferation and apoptosis of tumor cells. A multivariate analysis of disease-free survival demonstrated that p53 accumulation and bax expression are not significant independent indicators of prognosis in operable breast carcinoma. Our results also show that the proportion of bax- and p53-positive cells does not vary between primary and metastatic tumors. Conclusions: p53 accumulation and loss of bax expression influence the acquisition of a malignant phenotype but seem to have no further impact on tumor progression.

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Section: Discussionsupporting

confidence: 92%

Expression of bax and p53 Proteins in the Tumorigenesis and Progression of Breast Carcinomas

Redondo¹,

Garcia²,

Rodrigo³

et al. 2003

Tumor Biol

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“…For example, the AIs are greater in lymph node metastases than in primary breast carcinomas [18], and AIs are also positively correlated with the pathologic grades of gliomas [19]. AI elevation has also been observed in the carcinogenesis of the endometrium [20] or the lung [21]; according to the observations of Törmänen et al, the demonstrated AI in these contexts also increased with the severity of the dysplasias that were observed.…”

Section: Discussionmentioning

confidence: 99%

The role and prognostic value of apoptosis in colorectal carcinoma

et al. 2013

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BackgroundAlterations to apoptosis are a common occurrence in human tumours. The aim of our study was to determine the influence of apoptotic variations on the carcinogenesis and prognosis of colorectal carcinomas (CRCs).MethodsA TUNEL assay was performed on archival material from 103 colorectal carcinomas, 26 adenomas and 20 samples of normal epithelia.ResultsThe number of apoptotic cells was higher in CRCs (1.09 ± 0.13) than in adenomas (0.38 ± 0.23, p = 0.059) and normal epithelium (0.06 ± 0.04, p = 0.001). In addition, the apoptotic index (AI) was greater in metastatic disease (stage IV) than in other stages (p = 0.017). No relationship was found between apoptotic rates and age, gender or tumour grade. However, patients with tumours that showed higher AI values had a significantly lower disease-free survival (DFS) and overall survival (OS) than those with tumours that had lower AIs (p = 0.020 and p = 0.027). In a multivariate Cox proportional hazards model, AI remained a significant independent predictor of survival.ConclusionsWe conclude that disregulated apoptosis is an important event during CRC development and progression. Higher AIs are associated with more aggressive tumours and a poorer prognosis for patients with CRC.

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“…Bcl-2 and clusterin expression was scored as negative if no staining was seen, and positive if more than 10% of tumor cells showed staining. The selection of this cutoff value for clusterin and bcl-2 is based on previous reports [6,11,18] .…”

Section: Histology and Immunohistochemistrymentioning

confidence: 99%

“…However, the prognostic significance of bcl-2 protein expression in tumor pathology is contradictory. Some authors have suggested a favorable prognostic role of bcl-2 positivity in breast and non-small cell carcinomas [11][12][13] while others have found a direct relationship between bcl-2 protein expression and a worse prognosis in prostate carcinomas and meningiomas [14,15] . However, the prognosis of bcl-2 expression has been reported in laryngeal carcinoma though with variable results [9,10,16] .…”

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confidence: 99%

Expression of the Antiapoptotic Proteins Clusterin and Bcl-2 in Laryngeal Squamous Cell Carcinomas

Redondo¹,

Esteban²,

González‐Moles³

et al. 2006

Tumor Biol

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Bcl-2 and clusterin genes have been related to the inhibition of apoptosis, an event that plays a key role in malignant transformation and in invasive disease. In this work, we determine the significance of clusterin and bcl-2 expression in a large series of laryngeal carcinomas. We used immunohistochemical methods and in situ hybridization to examine the expression of these proteins. Nontumoral epithelial laryngeal tissues did not express clusterin and bcl-2 proteins. However, 9% (14 out of 154) and 25% of these tumors (39 of 154) had positive clusterin and bcl-2 staining, respectively. Clusterin expression was significantly related to the degree of local invasion and higher bcl-2 expression was found in these clusterin-positive tumors (p < 0.05). Bcl-2 expression was significantly correlated with supraglottic localization, nodal metastases, invasion in depth, and poorly differentiated tumors. However, by multivariate analysis, bcl-2 was shown to be an independent predictor of good prognosis in these tumors (OR = 0.12, 95% CI = 0.02–0.91). These findings indicate that clusterin and bcl-2 are upregulated in laryngeal carcinomas and their expression is related to the invasiveness of these tumors.

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<i>bcl-2</i> Expression and Apoptosis in Primary and Metastatic Breast Carcinomas

Cited by 29 publications

References 30 publications

Expression of bax and p53 Proteins in the Tumorigenesis and Progression of Breast Carcinomas

Expression of bax and p53 Proteins in the Tumorigenesis and Progression of Breast Carcinomas

The role and prognostic value of apoptosis in colorectal carcinoma

Expression of the Antiapoptotic Proteins Clusterin and Bcl-2 in Laryngeal Squamous Cell Carcinomas

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