<i>Dendrobium nobile</i> Alkaloids Protects against H₂O₂-Induced Neuronal Injury by Suppressing JAK–STATs Pathway Activation in N2A Cells

Abstract

The purpose of this study was to investigate the preventive effect and mechanism of Dendrobium alkaloids (DNLA) on oxidative stress-related death in neuronal cells. Our results demonstrated that DNLA has a direct neuroprotective effect through oxidative stress in N2A cells induced by hydrogen peroxide (H 2 O 2). CCK8, lactate dehydrogenase (LDH), intracellular Ca 2 , intracellular reactive oxygen species (ROS), and mitochondrial membrane potential (MMP) were used to evaluate the mechanism of DNLA neutralization by H 2 O 2-induced injury. Results presented in the paper indicate that treatment with DNLA (35 ng/mL) significantly attenuated decreases in cell viability, release of LDH, and apoptosis after H 2 O 2-induced neuronal injury. Furthermore, DNLA significantly reduced intracellular Ca 2 up-regulation, ROS production, and inhibited mitochondrial depolarization. Moreover, DNLA treatment significantly downregulated expressions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-6, nitric oxide synthase, janus kinase-signal transducer and activators of transcription (JAK-STATs) signaling in N2A cells, all of which were H 2 O 2-induced. Taken together, our findings suggested that DNLA may inhibit the expression of pro-inflammatory and proapoptotic factors by blocking JAK-STATs signaling after oxidative stress injury. This research provides a potential experimental basis for further application of DNLA to prevent various human nervous system diseases caused by oxidative stress.

Efficacy of the Nourishing Yin and Clearing Heat Therapy Based on Traditional Chinese Medicine in the Prevention and Treatment of Radiotherapy-Induced Oral Mucositis in Nasopharyngeal Carcinomas: A Systematic Review and Meta-Analysis of Thirty Randomized Controlled Trials

“…Our study found 13 Chinese herbal medicines with a high frequency of application in NYCH therapy ( Table 2 ). It was revealed that the main active ingredients in all 13 medicines [ 17 , 27 , 49 – 61 ] included flavonoids, terpenoids, steroids, sterols, coumarins, and emodin, which have anti-inflammatory, analgesic, and wound healing effects. The anti-inflammatory mechanism may be related to the inhibition of signaling pathways such as nuclear factor kappa-B (NF- κ B), mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase-Akt (PI3K-Akt), Janus kinase-signal transducers, and activators of transcription (JAK-STAT) [ 62 – 69 ].…”

Variability of Volatile Compounds in the Medicinal Plant Dendrobium officinale from Different Regions

“…Polysaccharides of D. officinale exhibit anti-aging, immune-enhancing, anti-inflammatory, liver-protective and anti-tumor activities [2]. The biological activities of alkaloid have been proved anti-oxidation, treat cell damage, anti-inflammation, liver-protection, anti-neurodegeneration and so on [3,4]. The medicinal value of D. officinale is recognized by the public and market demand has been steadily on the increase, but wild D. officinale usually grows on nutrient-poor cliffs or tree trunks on mountains close to one kilometer above sea level and prefers to grow on wet rocks, indicating that it has very strict requirements for its habitats, and mainly distributed in Southwest Anhui, Eastern Zhejiang, Jiangxi, Western Fujian, Northwest Guangxi, Sichuan and Southeast Yunnan [5].…”

Free radical scavengers from dendrobium virgineum

“…ex Lindl. เอื ้ องผึ ้ งน้ อย Ueang phueng noi (Chiang Mai) D. kanburiense Seidenf.activator of transcription signal, resulting in an alleviation of inflammation after oxidative injuries(Liu, Zhu, et al 2020). The mechanism of those properties is associate with reduced ROS.Figure 1 Dendrobium virgineum Rchb.f.…”