<i>Euphorbia royleana</i>, a botanical pesticide affects ultimobranchial gland of the catfish <i>Heteropneustes fossilis</i>

Abstract

Heteropneustes fossilis were subjected to high and low doses of the latex of Euphorbia royleana for short-term and long-term exposures, respectively. The blood was analyzed for plasma calcium levels, and the ultimobranchial glands fixed and examined. Serum calcium levels declined after 48 h following short-term exposure, and this decrease continued until the end of the experiment. The ultimobranchial cells exhibited a decrease in the cytoplasmic staining response after 96 h following treatment, and the nuclear volumes slightly decreased. Chronically exposed fish also exhibited a decline in serum calcium levels, but much later, on day 7, again progressively declining until the close of the experiment. Up to day 14 following treatment there was no change in histological structure of the ultimobranchial glands, but then there was a decrease in nuclear volume and the cytoplasm displayed a weak staining response. There was some vacuolization and degeneration.

Microcystin-LR Affects Ultimobranchial Gland of Catfish Heteropneustes fossilis

“…Earlier to the present study, there exists no study regarding the effect of Microcystin on the fish UBG. The observed inactivity and degeneration of UBG in treated fish derives support from the similar observations reported by other investigators after exposure of the fish to different toxicants-deltamethrin (Srivastav et al, 2002), metacid (Mishra et al, 2004), cypermethrin (Mishra et al, 2005), cadmium (Rai et al, 2009), botanical pesticide (Prasad et al, 2011a;2011b;Kumar et al, 2013) and mercury (Agarwal, 2013). Inactivity with prominent degenerating changes in UBG cells were reported by (Srivastav et al, 2019) in frog Euphlyctis cyanophlyctis.…”

Alterations in the Corpuscles of Stannius of Euphorbia royleana Treated Catfish, Heteropneustes fossilis

“…"Many plant species from different families which possess number of compounds such assaponins, tannins, alkaloids di-and tri-terpenoids have been used for control of harmful snails, disease-causing insects such as mosquito larvae" [15][16][17][18][19][20][21] . The effects of Euphorbia royleana plant were studied on the histopathological parameters of catfish H. fossilis [22,23].…”