<i>Ex Vivo</i> Model for Percutaneous Vertebroplasty

Abstract

Add to CartThe testing of novel biomaterials for percutaneous vertebroplasty depends on suitable animal models. The aim of this study was to develop ex vivo a reproducible and feasible model of percutaneous vertebroplasty, for ulterior application in vivo. A large animal model was used (Merino sheep), due to its translational properties. Vertebroplasty was performed under tactile and fluoroscopic control, through a bilateral modified parapedicular access in lumbar vertebrae (n=12). Care was taken in order to avoid disruption of the vertebral foramen. The average defect volume was 1234±240 mm 3

Percutaneous vertebroplasty in sheep: testing a novel mesoporous bioactive glass/calcium sulphate cement

“…and were then combined with water inside a syringe using an optimized liquid to powder ratio (L/P=0.4ml/g), to obtain an injectable paste. Prior to the in vivo study, the cement went through bioactivity and resorbability, ex vivo injection, mechanical and in vitro testing [13,14]. All the tests were carried out using a commercial reference as control, prepared according to the manufacturer's instructions (Cerament™).…”

“…All underwent PVP under general anaesthesia. Vertebral body V-shaped defects were created in the cranial hemivertebrae, through a modified parapedicular approach developed by the authors [14,16]. Cements were injected under fluoroscopic guidance using a bone-filler system device (Medtronic Spine LLC, Portugal) and set for two hours in the anaesthetized sheep.…”

“…Prior to the in vivo study, the cement went through bioactivity, resorbability, ex vivo injection, mechanical, and in vitro testing, all of which with favorable results [13,14]. The experimental cement could be easily injected without interruption, and it showed a satisfactory radiopacity.…”