<i>Hordeum vulgare</i> miežių lapų stabilių DNR baltymų kompleksų baltymų sudėties analizė

Abstract

Tightly bound to DNA proteins (TBP) are a protein group that remains attached to DNA with covalent or non-covalent bonds after its deproteinisation [1,2]. TBP have been found in DNA of numerous evolutionary distant species [3][4][5]. Some of these TBP proteins have been characterized in Ehrlich ascites [6-8] and yeast (Saccharomyces cerevisiae) [9] cells. The aim of this work was to characterize TBP proteins in the first leaves of barley (Hordeum vulgare) shoots by the MALDI TOF-TOF mass-spectrometry (MS) analysis. We have identified that most of these proteins (WRKY transcription factors 16 and 52, MADS-box transcription factor 26, Squamosa promoter-binding-like protein 16, Scarecrow-like protein 9, TGA4, TEOSINTE BRANCHED 1) are transcription factors playing important roles in the development and coping with either biotic or abiotic stress in plants. Various barley TBP (above transcription factors, DEMETER-like protein 2, HAC12, RAD51, Ty3-gypsy retrotransposon, protein kinases, serpins) participate in chromatin rearrangement and the regulation of gene expression.

Tightly bound to DNA proteins: Possible universal substrates for intranuclear processes

“…Our results confirm the presence of serpins, kinases and phosphatases in TBPs isolated from different sources (Bagdonienė et al, 2008;Bielskiene et al, 2009). The protein encoded by retrotransposon Ty3-gypsy appeared to be specific for plant TBPs ).…”

Identification of QTL Associated with Regrowth Vigor Using the Nested Association Mapping Population in Switchgrass

“…Among these genes, Probable indole-3-pyruvate monooxygenase YUCCA10 has been reported to be involved in auxin synthesis, which affects leaves and flower formation [52]. Scarecrow-like protein 9 is known to play an important role in development and to cope with either biotic or abiotic stress in plants [53]. F-box protein SKIP28 is considered to play a variety of roles in developmental processes, including plant hormonal signal transduction, secondary metabolism, senescence, and responses to both biotic and abiotic stresses [54].…”