&lt;i&gt;In vitro&lt;/i&gt; and &lt;i&gt;in vivo&lt;/i&gt; hepatoprotective activity of extracts of aerial parts of &lt;i&gt;Bidens pilosa&lt;/i&gt; L (Asteraceae)

Abdel-Ghany, Rasha; Barakat, Waleed; Shahat, Abdelaaty A.; Abdallah, Walid E.; Ali, Elzahraa Atef

doi:10.4314/tjpr.v15i11.11

Cited by 10 publications

(7 citation statements)

References 14 publications

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“…This difference could be attributed to the increased sensitivity of the xCELLigence compared to the MTT assay or the use of different solvents, i.e., we used ethanol instead of methanol. In contrast to the absence of EC 50 values (no cytotoxicity observed) for the water extracts in the current study, Abdel-Ghany et al (2016) [75] published EC 50 of 1000 µg/ml after exposure of aqueous extracts of B. pilosa to primary cell cultures (rat hepatocytes). These authors used primary cell cultures (short life span) in contrast to the immortal cell lines used in the current study.…”

Section: Cytotoxicity Using Rtcacontrasting

confidence: 90%

Phytochemical-Based Evidence of the Health Benefits of Bidens Pilosa Extracts and Cytotoxicity

et al. 2023

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Purpose Bidens pilosa L. is traditionally used as a flavouring agent in foods, in the treatment of diseases, in agriculture as a biopesticide and herbicide, and in the phytoremediation of soils contaminated with heavy metals. The vast range of uses of B. pilosa for a variety of purposes is questionable, hence motivating the objectives of this study, which are to assess the cytotoxicity, health benefits, and/or risks of B. pilosa using chemical-based evidence. Methods A real-time cell analysis (xCELLigence system), ultra-high-performance liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometer, high-performance liquid chromatography with a diode array detector, and chemical-based spectrophotometric methods were adopted in the study. Results High concentrations of the ethanol extracts exhibited cytotoxic activity on HepG2 (cancerous), and Vero (non-cancerous) cell lines, whereas the water extracts promoted cell proliferation at selected concentrations. The chemical profiling enabled the separation as well as characterisation of 137 phytochemicals. These were mainly phenolic acids, flavonoids, fatty acids, coumarins, and furanocoumarins. There was no toxic compound identified. Conclusion The ethanol extracts are generally more potent and exhibit stronger antioxidant activity and cytotoxicity, probably due to the presence of more flavonoids and phenolic acids, validating the uses of B. pilosa and its relevance as a source of functional phytochemicals.

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Section: Cytotoxicity Using Rtcacontrasting

confidence: 90%

Phytochemical-Based Evidence of the Health Benefits of Bidens Pilosa Extracts and Cytotoxicity

et al. 2023

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“…Results of this study are in agreement with those from previous studies of Chaudhari and Mahajan [8] and Abdel-Ghany et al [26] that showed severe liver damage (assessed by significant elevation in ALT, AST, ACP, LDH and ALP levels) following administration of CCl4. ALT is considered more specific for hepatic disorders because it is mainly present in the cytosol of hepatocytes but in low concentration elsewhere.…”

Section: In Vitro Hepatoprotective Activitysupporting

confidence: 93%

“…Reactive free radicals initiate cell damage through the following 2 major mechanisms: Covalent binding to cell membrane lipids and lipid peroxidation. In addition, oxidative stress may cause reversible or irreversible alterations of sensitive proteins that may lead to increased susceptibility to proteolytic attacks [26]. Lipid peroxidation and free radicals are involved in the main mechanisms by which hepatotoxins injure hepatocytes.…”

Section: In Vitro Hepatoprotective Activitymentioning

confidence: 99%

“…In addition, the oxidative stress caused by CCl4 has been shown to induce mitochondrial dysfunction and depletion of adenosine triphosphate. Abdel-Ghany et al [26] reported that CCl4 reduces glutathione content by increasing lipid peroxide generation. Furthermore, the enzymes that scavenge free radicals, such as glutathione reductase, peroxidase, and catalase are decreased during the induction of cirrhosis.…”

Section: In Vitro Hepatoprotective Activitymentioning

confidence: 99%

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In Vitro Hepatoprotective Activity of Methanolic Leaf Extract of Acalypha indica Against CCl4 Induced Hepatotoxicity in Goat Liver Slice Culture

Nambiar

Varghese²

2023

Trends Sci

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Plants used in traditional medicine may constitute an important source of new biologically active compounds. Acalypha indica is one of the weed plants that contain important medicinal values for human health applications. The present study was undertaken to assess the hepatoprotective activity of methanolic leaf extract of Acalypha indica in goat liver slice culture against carbon tetrachloride (CCl4) to prove its efficacy against liver disorders CCl4 was used to induce hepatotoxicity in liver slice of goat. The cytotoxicity induced by CCl4 was estimated by quantifying the release of marker enzymes such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), acid phosphatase (ACP) and lactate dehydrogenase (LDH). Also, the degree of hepatic damage was measured by estimating the levels of lipid peroxidation (LPO) of hepatocytes membrane lipids. The treatment of liver cells with CCl4 caused twice increase in LPO of cells besides release of LDH, ALT, AST, ACP and ALP by 6.55, 3.89, 3.31, 2.69 and 2.50 times, respectively as compared to untreated liver cells. Thus, a toxic effect of CCl4 was significantly reduced by the treatment of methanolic leaf extract of Acalypha indica, silymarin and quercetin. The free radical scavenging activity of plant extract was estimated by 2, 2-diphenylpicrylhydrazyl (DPPH) method and IC50 is 65.12 ± 2.27 μg/mL. Qualitative analysis of flavonoid indicates the presence of flavonoids in plant extract. These results indicate that all concentration of plant extract of Acalypha indica, silymarin and quercetin protect the liver cells from CCl4 induced oxidative/free radical mediated damage in vitro. The hepatoprotective activity of methanolic extract of Acalypha indica extract is due to the presence of flavonoids which have remarkable antioxidant and anti-inflammatory activity. HIGHLIGHTS Liver disorders are the most common health hazard found in developing countries due to dietary habits, alcohol ingestion, poor hygiene, unsupervised drug use and smoking. Liver diseases can be non-inflammatory, inflammatory and degenerative Acalypha indica is one of the weed plants that contain important medicinal values for human health applications In vitro study of methanolic leaf extract of Acalypha indica against CCl4 induced hepatic damage in goat liver slice culture proven its efficacy against liver diseases GRAPHICAL ABSTRACT

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“…There are a growing number of studies purporting hepatoprotective effects with traditional medicines, and based on claims in folkloric use, several medicinal plants have been evaluated scientifically with promising hepatoprotective activity. These plants include Adansonia digitata [ 1 ], Alysicarpus vaginalis [ 2 ], Bidens pilosa [ 3 ], Brassica oleracea [ 4 ], Cineraria abyssinica [ 5 ], Indigofera barberi [ 6 ], Justicia schimperiana [ 7 ], Nigella sativa [ 8 ], Opuntia monacantha [ 9 ], Verbascum sinaiticum [ 7 ], Vicia calcarata [ 10 ], Ziziphus jujuba [ 11 ]. In recent years, hepatoprotective natural antioxidants and compounds with radical-scavenging activity have been identified, including curcumin and ginger [ 12 ], Hibiscus species [ 13 ] Polyalthia longifolia [ 14 ] and Cassia italic [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of hepatoprotective and antidiarrheal activities of the hydromethanol crude extract and solvent fractions of Schinus molle L. (Anacardiaceae) leaf and fruit in mice

Belayneh,

Mengistu,

Hailay

2024

Metabolism Open

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<i>In vitro</i> and <i>in vivo</i> hepatoprotective activity of extracts of aerial parts of <i>Bidens pilosa</i> L (Asteraceae)

Cited by 10 publications

References 14 publications

Phytochemical-Based Evidence of the Health Benefits of Bidens Pilosa Extracts and Cytotoxicity

Phytochemical-Based Evidence of the Health Benefits of Bidens Pilosa Extracts and Cytotoxicity

In Vitro Hepatoprotective Activity of Methanolic Leaf Extract of Acalypha indica Against CCl4 Induced Hepatotoxicity in Goat Liver Slice Culture

Evaluation of hepatoprotective and antidiarrheal activities of the hydromethanol crude extract and solvent fractions of Schinus molle L. (Anacardiaceae) leaf and fruit in mice

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