<i>In-vitro</i> Antimicrobial and Antitumor Activities of <i>Stevia Rebaudiana</i> (Asteraceae) Leaf Extracts

Abstract

Purpose:The purpose of the study is to evaluate the antimicrobial and antitumor activities of Stevia rebaudiana (Asteraceae) leaf extracts. Methods: Four solvent extracts (ethyl acetate, acetone, chloroform and water) of Stevia rebaudiana leaves were investigated against Staphylococcus aureus, Salmonella typhi, Escherichia coli, Bacillus subtilis, Aeromonas hydrophila and Vibrio cholerae by using agar well diffusion method. Candida albicans, Cryptococcus neoformans, Trichophyton mentagrophytes and Epidermophyton species were used to test anti-yeast and antifungal activity. The cytotoxic effects of the extracts on Vero and HEp2 cells were assayed using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide [MTT]. Results: Among the four extracts tested, acetone extract had effective antibacterial potential, followed by ethyl acetate extract. The acetone extract showed greater activity against Gram-positive than against Gram-negative organisms. All the extracts were active against Epidermophyton species and Candida albicans. The 1:8 dilution of the acetone extract was non-toxic to normal cells and also had both anticancer and anti-proliferative activities against cancerous cells. Conclusion: The study confirms the antimicrobial and antitumor activities of Stevia rebaudiana leaves extracted using various solvents, and is therefore, a potential drug that requires further studies and development.

The Effects of Rebaudioside A on Microbial Diversity in Mouse Intestine

“…1-D). These results were also observed by Ghosh et al and Jayaraman et al who found that various solvent extracts of Stevia rebaudiana leaves could inhibit some bacteria and fungal, but no explanation was provided (Ghosh et al, 2008;Jayaraman et al, 2008). In addition, some researchers mentioned that the secondary metabolite "Stevioside" of RA may be responsible for the antimicrobial activity (Debnath, 2008).…”

In vitro Propagation of Stevia rebaudiana (Bertoni): An Overview

“…The leaves of this plant has a pleasantly sweet and refreshing taste which is educe by diterpene glycosides (stevioside and rebaudiosides), a high-potency sweeteners and substitute to sugar, being 300 times sweeter than sucrose (Madan et al, 2010;Megeji et al, 2005;Singh and Rao, 2005;Soejarto et al, 1983;Soejarto DD, Kinghorn AD, 1982;Yadav et al, 2011). Stevia is a well-known therapeutic agent serve as an efficient medication for diabetes, hypertension, myocardial and antimicrobial infections, dental troubles, and tumors (Chan et al, 1998;Gregersen et al, 2004;Jayaraman et al, 2008;Jeppesen et al, 2003Jeppesen et al, , 2002Kujur et al, 2010;Marcinek and Krejpcio, 2016;Muanda et al, 2011;Philippe et al, 2014;Planas and Kucacute, 1968;Shivanna et al, 2013;Šic Žlabur et al, 2013;Singh et al, 2015). In present time people are very calorie conscious which increases use of stevia in preparation of non-calorie food stuffs and become a major sweetening agent in food products in South-east Asia (Ashwell,billion US dollar.…”

Profile and Pro-Health Properties of Stevia Rebaudiana Bertoni

“…Jayaraman i wsp. [16] analizowali właściwości czterech ekstraktów liści stewii. Najbardziej efektywny w przypadku bakterii był ekstrakt acetonowy (zwłaszcza w odniesieniu do bakterii G+), następnie ekstrakt na bazie octanu etylu.…”