<i>In vitro</i> antimicrobial screening of methanolic extracts of <i>Cleome chelidonii</i> and <i>Cleome gynandra</i>

Abstract

Antimicrobial activity of methanolic extracts of plants was screened by disc diffusion assay against four bacteria and four fungal cultures. Streptomycin (10 µg/disc) and nystatin (10 µg/disc) are used as standards for bacteria and fungi respectively. Minimum inhibitory concentration (MIC) of the extracts was evaluated through micro broth dilution method. The antimicrobial potency of plant extracts was assessed by their zone of inhibition and activity index values. Total activity of extracts was evaluated to quantitatively compare the activity of two plants. Methanolic extract of Cleome gynandra showed maximum antibacterial activity against Staphylococcus aureus (IZ-22 ± 0.22 mm, AI-0.917, MIC-0.039 mg/mL, MBC-0.039 mg/mL). Maximum antifungal potential was shown by C. chelidonii against Candida albicans (IZ-25 ± 0.92 mm, AI-1.000, MIC-0.039 mg/mL, MFC-0.039 mg/mL). Both the extracts exhibited good antimicrobial activity with low range of MIC.

New distributional records of Cleome chelidonii L.f. and Cleome rutidosperma DC. (Cleomaceae) in Madura Island

“…Even so, the species has potential as a medicinal plant. Further pharmacological studies revealed that the methanol extract of C. chelidonii has antimicrobial, antiinflammatory, antinociceptive, and antipyretic activities (Parimalakrishnan et al, 2007;Sridhar et al, 2014). Moreover, the flavonoids isolated from the leaf extract were also observed to have hepatoprotective activity and cytotoxicity against the HepG2 human hepatoma cell line (Nguyen et al, 2017).…”

Mineral and Phytochemical Composition of Cleome Gynandra Methanolic Extract

“…These plants phytochemicals with pharmacological properties are mainly secondary metabolites of the plant's cellular metabolism. Cleome gynandra was found to contain a number of phytochemical secondary metabolites through qualitative chemical analysis and they include saponnins, flavonoids, tannins, alkaloids, cardiac glycosides, terpanoids and polyphenols [17]. The plant also had several sulfur or thiol compounds that could probably have the effect of scavenging of free radicals in cells by regenerating reduced glutathione or by direct interaction with the free radicals, therefore have antioxidant function, this were discovered by GCMS activity this include the thiol compound 2-nhexylthiol-5-methyl-imidazole.…”

“…When sapponins are injected directly into the blood of animals they cause extravascular haemolysis of erythrocytes due to their saponification effect on cell membranes. Spider plant also contains alkaloids which have been found to act as stimulants while some have sedative effect [17,19]. Some bioactive compounds detected by Gas Chromatography Mass Spectroscopy (GSMS) method include benzoic acid derivatives have been found to have antimicrobial activity while phenol derivatives have Anti-microbial, Analgesic Anti-oxidant, Antiinflammatory properties.…”

Effects of Cooking and Drying on the Total Phenolic, Total Flavonoid Content, Antioxidant and Antibacterial Activity of Cleome gynandra (Spider Plant)

“…It has been utilised traditionally to treat stomach aches, headaches [11], ear diseases [12], food poisoning, rheumatism, sexual asthenia, antibacterial infections and snake bites [13]. Some studies have also shown its ability to act as an antioxidant [14], [15], [13], [16], [10], [4] while some have shown its antibacterial properties [17], [18]. Such health benefits could be attributed to its phenolic composition that has been reported in earlier studies [13], [19], [20], [21], [22], [23].…”