<i>In vitro</i> antioxidant activities of the fractions of </i>Coccinia grandis</i> l. leaf extract

Abstract

The present study was aimed at investigating the antioxidant activities of the various fractions of the hydromethanolic extract of the leaves of Coccinia grandis L. Voigt. (Cucurbitaceae). The antioxidant activities of the fractions have been evaluated by using nine in vitro assays and were compared to standard antioxidants such as ascorbic acid, α-tocopherol, curcumin and butylated hydroxyl toluene (BHT). All the fractions showed effective Hdonor activity, reducing power, free radical scavenging activity, metal chelating ability and inhibition of β-carotene bleaching. None of the fractions exerted an obvious pro-oxidant activity. The antioxidant property depends upon concentration and increased with increasing amount of the fractions. The free radical scavenging and antioxidant activities may be attributed to the presence of phenolic and flavonoid compounds present in the fractions. The results obtained in the present study indicate that the leaves of C. grandis are a potential source of natural antioxidant.

Comprehensive Evaluation of Antioxidant Potential of Selected Osbeckia species and their in vitro Culture, Purification and Fractionation

“…Umamaheswari and Chatterjee analyzed in vitro varied antioxidant of the different solvent fractions of Coccinia grandis leaf extracts. 21 The present AOX potential of Osbeckia species are comparable with the above results. Purified anthocyanin of both the species showed remarkable activity ie 68.5 and 63.4 percentage respectively at 25 µg/ml (Table 4).…”

“…20 Umamaheswari and Chatterjee analyzed in vitro antioxidant activities of the fractions of Coccinia grandis leaf extract. 21 Saito et al extensively activity is dependent on the chemistry of anthocyanins and not all of them show similar activities for scavenging ROS and RNS. The antioxidant ability of anthocyanins depends on the basic structural orientation of the compound because the ring orientation will decide the way by which a hydrogen atom from a hydroxyl group can be donated to a free radical as well as the potentiality of the anthocyanin to support an unpaired electron.…”

Chemo-profiling, Antioxidant Potential and Ionomic Analysis of Cichorium intybus L.

“…In plants reducing power assay has been considered as one of the most important indicator of antioxidant activity. 49 The presence of reductones in the plant extract is commonly correlated with its reducing capabilities as reductones have shown the antioxidant action by terminating the radical chain reaction as they donates hydrogen atom in the reaction mixture. 50 In our study, it has been observed that the total reduction ability of converting Fe 3+ to Fe 2+ in presence of both the extracts increases in a concentration dependent manner suggesting that both the extracts donates an electron in the reaction mixture which reacts with free radicals breaking down the chain reaction and transform them into much stable non-radical products.…”

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“…Antioxidants fight against free radicals and defend us from various diseases. They exert their action either by scavenging the reactive oxygen species or protecting the antioxidant defense mechanisms 28 . The electron donation ability of natural products can be measured by 2, 20 -diphenyl -1 -picryl hydrazyl radical (DPPH) purple-coloured solution bleaching 9 .…”