<i>In Vitro</i> Antioxidant and <i>In Vivo</i> Hepatoprotective Activity of Leave Extract of <i>Raphanus Sativus</i> in Rats Using CCL₄ Model

Abstract

Background: Raphanus sativus is reported to have a variety of biological activities. This work screened the hepato-protective and antioxidant activity of ethanol (ERS), and aqueous (ARS), extracts of leaves of Raphanus sativus in Carbon tetrachloride (CCl 4 ), model in rats. Material and Methods: The extracts were subjected to antioxidant tests (Total reducing power and Total phenolic content), and preliminary phytochemical screening. A pilot study was done on 100 and 300 mg/kg extracts, form which 300 mg was chosen for further experiments. The albino rats (200-250 grams), were divided into 5 groups of 6 animals each (n=6). There were three control groups comprising of normal control (normal saline -1ml/kg), negative control group (CCl 4 1ml/kg in olive oil in a ratio of 1:1 v/v), and positive control group (Silymarin 50mg/kg). The Test drugs were given in a dose of 300 mg/kg for both ERS and ARS extract for 7 days. Biochemical parameters (AST, ALT, Alkaline phosphatase, Total Bilirubin), histo-pathological examination of liver and in vivo antioxidant tests [CAT, GSH and MDA] were done. Results: The phytochemical study showed the presence of flavanoids, terpenoids, alkaloids, saponins and sterols. A dose dependent increase in the oxidative potential was observed in both the extracts with total phenolic content 70.1 and 44.4 GAE/g extract for ERS and ARS respectively. ERS 300mg/kg showed a significant (p<0.001) increase in levels of AST, ALT and alkaline phosphatase as compared to negative control (percentage hepatoprotection =45.3%) while ARS 300 mg/kg (p<.01) group showed 30% hepatoprotection. The GSH (p<0.001) and CAT (p<0.05) in ERS and ARS were significantly increased while MDA levels were decreased (P< 0.01), as compared negative control. The findings were confirmed histo-pathological examination. Conclusion:The ethanol and aqueous extract of Raphanus sativus have partial hepatoprotection against CCl 4 toxicity.

Protection efficiency of red radish seeds and roots against paracetamol mediated hepatotoxicity in rats

“…In the present study crude ethanol extracts of red radish seeds and roots were rich in phenolic compounds. The present results are in agreement with the results of Syed et al (29) who reported that phenolic content of radish ethanol extract was 70.1 GAE/g of extract. Due to radish content of the bioactive compounds that have antioxidant activity, it has been postulated that radish has a positive role in protecting the liver from oxidative damage and hepatotoxicity.…”

“…Allyl isothiocyanate has been shown to protect the liver from the toxicity caused by carbon tetrachloride. Also Syed et al (29) reported that radish contains quercetin, polyphenols and anthocyanins by which radish can ameliorate the elevations in MDA and NO, reduce the oxidation through increasing the activities of antioxidant enzymes (glutathione S-transferase and superoxide dismutase) and suppress the stimulants of oxidation (nitric oxide synthase, cyclooxygenase 2, lipoxygenase 2 and xanthine oxidase). Adeneye and Olagunju (31) and Modo et al (32) disclosed that vitamin C showed protective role against paracetamol mediated hepatotoxicity.…”

Tangeretin Alleviates Cisplatin-Induced Acute Hepatic Injury in Rats: Targeting MAPKs and Apoptosis

“…Several studies have reported its hepatoprotective effects in acute and chronic hepatic diseases [ 24 – 26 ]. Silymarin has been reported to protect against CCl4- [ 27 – 29 ], acetaminophen- [ 30 , 31 ], D-galactosamine- [ 32 ] and cisplatin- [ 33 ] induced liver injuries.…”

Fraxin Prevents Chemically Induced Hepatotoxicity by Reducing Oxidative Stress

“…According to Wang et al, Fraxin was orally administered to mice at 500 mg/kg and no toxicity was observed [ 13 ]. The positive controls were gavaged with silymarin (50 to 100 mg/kg) in CCl 4 induced hepatotoxicity rats [ 4 , 14 , 15 ]. We sought to evaluate the hepatoprotective effect of fraxin on the same dose as that of the positive control.…”