<i>In vitro</i> antitumor effects of a new cultivar (Gürarslan) of <i>Trigonella foenum graecum</i> l.

Abstract

Background: The main goals of our study were as follows: (1) to investigate whether the methanolic extract of the new cultivar (Gürarslan) of Trigonella foenum graecum L. seeds exhibit antiproliferative effects on the HeLa human cervix carcinoma, Mat-LyLu rat prostate carcinoma and 3T3 embryonic mouse fibroblast cell lines, (2) to determine how mitosis and DNA synthesis are affected in cancer cells by the extract, and (3) to observe the changes in cell morphology after treatment with the extract. Materials and Methods: The effect on cell proliferation of these extracts was detected by using methyl thiazolyl diphenyl tetrazolium (MTT) assay. Mitotic index and labelling index was determined using the Feulgen staining and autoradiography methods, respectively. Results: Our findings show that the methanolic extract of T. foenum graecum seeds might have antiproliferative properties on the cancer cell lines only, but not on the 3T3 embryonic mouse fibroblast cell line. Conclusion: Our data show that the new variety of T. foenum graecum may have antitumoral properties.

Cytotoxic activity of standardized extracts, a fraction, and individual secondary metabolites from fenugreek seeds against SKOV-3, HeLa and MOLT-4 cell lines

“…In our study, the cytotoxic activity of T. foenum-graecum against MOLT-4 and SKOV-3 cell lines was evaluated for the first time. The obtained IC 50 values​ against the HeLa cell line were significantly lower than the IC 50 value determined by Aktas and Altun ( 2015 ) for methanol extract from T. foenum -graecum seeds (IC 50 = 84.0 ± 0.34 μg/mL).…”

“…The obtained IC 50 values against the HeLa cell line were significantly lower than the IC 50 value determined by Aktas and Altun (2015) for methanol extract from T. foenum -graecum seeds (IC 50 ¼ 84.0 ± 0.34 lg/mL). Tested steroidal sapogenins, 70% methanol extract (A) and aqueous extract (B), and the fraction C from Foenugraeci Semen did not show selective effect on the HeLa, SKOV-3 and MOLT-4 tumour cell lines compared to the control HaCaT keratinocytes line.…”

“…Moreover, Al-Oqail et al ( 2013), observed a toxic effect of fenugreek seed ether extract on control cell lines in addition to the cytotoxic effect on Hep-2 and MCF-7 cell lines. On the other hand, methanol and aqueous extracts of fenugreek seeds revealed selective cytotoxic activity, respectively on T-cell, B-cell lymphoma, papillary thyroid (FRO), breast cancer cell lines (Alsemari et al 2014) and HeLa, Mat-LyLu rat prostate cancer lines (Aktas and Altun 2015), without affecting control lines (human lymphocyte and 3T3 mouse fibroblast lines, respectively).…”