<i>In Vitro</i> Culture of BMSCs on VEGF-SF-CS Three-Dimensional Scaffolds for Bone Tissue Engineering

Abstract

The objective of the study was to discuss the biocompatibility of VEGF-silk fibroin-chitosan (VEGF-SF-CS) three-dimensional scaffolds in order to offer an ideal scaffold for bone tissue engineering. Bone marrow derived mesenchymal stem cells (BMSCs) were seeded on the VEGF-SF-CS three-dimensional scaffolds and silk fibroin-chitosan (SF-CS) three-dimensional scaffolds. On VEGF-SF-CS and SF-CS three-dimensional scaffolds, the cell adhesion rate was increased as time went on. Scanning electron microscopy: The cells grew actively and had normal multiple fissions, granular and filamentous substrates could be seen around the cells, and cell microfilaments were closely connected with the scaffolds. The cells could not only show attached growth on surfaces of the scaffolds, but could also extend into the scaffolds. CCK-8 and ALP analysis proved that the vascular endothelial growth factor (VEGF) could significantly promote BMSCs growth and proliferation in the SF-CS scaffolds; however, the enhancement of BMSCs cell proliferation and activity by VEGF is dependent on time. Thus, VEGF-SF-CS composite scaffolds represent a promising new type of scaffold for bone tissue engineering.

Withdrawal: Synthesis of a Novel bFGF/nHAP/COL Bone Tissue Engineering Scaffold for Mandibular Defect Regeneration in a Rabbit Model

“…The quality was recorded as m. The volume of the inner hole was recorded as Vp: Vp = (m − ms) / ρs. The porosity of scaff old was calculated as Vp / Vs. Each sample was measured three times and the average value was recorded 21) . Sample volume was recorded as V1, and then each sample was immersed in deionized water at room temperature for 24 h. The wet volume was recorded as V2.…”

Artificial Bone via Bone Tissue Engineering: Current Scenario and Challenges

“…When BMSCs cultured on VEGF-silk-fibroin-chitosan scaffolds showed significant cell attachment, cell proliferation compared to BMSCs cultured on silk-fibroin-chitosan scaffolds [63]. VEGFs incorporated PLGA scaffolds showed proliferation of endothelial cells and apatite formation revealing osteogenic and angiogenic potential [64].…”

“…Cells for bone tissue engineering BMSCs Osteoblast differentiation; osteoinduction; osteogenesis; mineralization; in vitro & in vivo bone regeneration[22,29,63,109,138,147,149,153] …”

Silk Fibroin-Based Scaffolds with Controlled Delivery Order of VEGF and BDNF for Cavernous Nerve Regeneration

“…Silk fibroin from Bombyx mori, an attractive biomaterial, has good biocompatibility, controlled degradability, nontoxicity, and versatile processability in different material formats. 9,10 Thus, silk fibroin has a wide range of applications in the biomedical fields such as bone, 11,12 skin, 13,14 low urinary tract, 15,16 and vascular 17,18 repair. Silk fibroin-based conduits also achieved considerable success for nerve regeneration.…”

“…Silk fibroin from Bombyx mori , an attractive biomaterial, has good biocompatibility, controlled degradability, nontoxicity, and versatile processability in different material formats. , Thus, silk fibroin has a wide range of applications in the biomedical fields such as bone, , skin, , low urinary tract, , and vascular , repair. Silk fibroin-based conduits also achieved considerable success for nerve regeneration. ,− Electrospun fiber-based scaffolds with the natural extracellular matrix (ECM) structures showed an increased cellular proliferation and exchange of nutrient/waste. − Compare to electrospun fiber scaffolds with random structures, those with aligned structures are capable of guiding neurite extension and Schwann cell (SC) migration .…”